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Literature summary for 3.6.1.56 extracted from

  • Waz, S.; Nakamura, T.; Hirata, K.; Koga-Ogawa, Y.; Chirifu, M.; Arimori, T.; Tamada, T.; Ikemizu, S.; Nakabeppu, Y.; Yamagata, Y.
    Structural and kinetic studies of the human Nudix hydrolase MTH1 reveal the mechanism for its broad substrate specificity (2017), J. Biol. Chem., 292, 2785-2794 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.1.56

Cloned(Commentary)

Cloned (Comment) Organism
-
 Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with 8-oxo-dGTPor 2-oxo-dATP, using 1.0 M sodium citrate, 0.1 M Tris-HCl, pH 7.0, and 0.2 M NaCl, or 1.0 M sodium citrate, 0.1 M cacodylate-HCl, pH 6.5, and 0.2 M NaCl Homo sapiens

Protein Variants

Protein Variants Comment Organism
D120A the mutant shows 8% of wild type activity Homo sapiens
D120N the mutant shows 4% of wild type activity Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
6-(2,3-dichlorophenyl)-N4-methylpyrimidine-2,4-diamine i.e. TH287 Homo sapiens
N4-cyclopropyl-6-(2,3-dichlorophenyl)pyrimidine-2,4-diamine i.e. TH588 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0052
-
 2-oxo-dATP wild type enzyme, at pH 7.5 and 22°C Homo sapiens
0.006
-
 2-oxo-dATP mutant enzyme D120N, at pH 7.5 and 22°C Homo sapiens
0.0084
-
 8-oxo-dGTP wild type enzyme, at pH 7.5 and 22°C Homo sapiens
0.0318
-
 2-oxo-dATP mutant enzyme D120A, at pH 7.5 and 22°C Homo sapiens
0.0412
-
 8-oxo-dGTP mutant enzyme D120N, at pH 7.5 and 22°C Homo sapiens
0.0444
-
 8-oxo-dGTP mutant enzyme D120A, at pH 7.5 and 22°C Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-oxo-dATP + H2O Homo sapiens
-
 2-oxo-dAMP + diphosphate
-
 ?
8-oxo-dATP + H2O Homo sapiens
-
 8-oxo-dAMP + diphosphate
-
 ?
8-oxo-dGTP + H2O Homo sapiens
-
 8-oxo-dGMP + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxo-dATP + H2O
-
 Homo sapiens 2-oxo-dAMP + diphosphate
-
 ?
8-oxo-dATP + H2O
-
 Homo sapiens 8-oxo-dAMP + diphosphate
-
 ?
8-oxo-dGTP + H2O
-
 Homo sapiens 8-oxo-dGMP + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
MTH1
-
 Homo sapiens
NUDT1
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.6
-
 8-oxo-dGTP mutant enzyme D120N, at pH 7.5 and 22°C Homo sapiens
9.7
-
 8-oxo-dGTP mutant enzyme D120A, at pH 7.5 and 22°C Homo sapiens
20.7
-
 8-oxo-dGTP wild type enzyme, at pH 7.5 and 22°C Homo sapiens
21.1
-
 2-oxo-dATP wild type enzyme, at pH 7.5 and 22°C Homo sapiens
21.1
-
 2-oxo-dATP mutant enzyme D120N, at pH 7.5 and 22°C Homo sapiens
34.4
-
 2-oxo-dATP mutant enzyme D120A, at pH 7.5 and 22°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.1
-
 8-oxo-dGTP mutant enzyme D120N, at pH 7.5 and 22°C Homo sapiens
0.2
-
 8-oxo-dGTP mutant enzyme D120A, at pH 7.5 and 22°C Homo sapiens
1.1
-
 2-oxo-dATP mutant enzyme D120A, at pH 7.5 and 22°C Homo sapiens
2.5
-
 8-oxo-dGTP wild type enzyme, at pH 7.5 and 22°C Homo sapiens
3.5
-
 2-oxo-dATP mutant enzyme D120N, at pH 7.5 and 22°C Homo sapiens
4.1
-
 2-oxo-dATP wild type enzyme, at pH 7.5 and 22°C Homo sapiens