BRENDA - Enzyme Database show
show all sequences of 3.6.1.52

Oxidation of the diphosphoinositol polyphosphate phosphohydrolase-like Nudix hydrolase Aps from Drosophila melanogaster induces thermolability--A possible regulatory switch?

Winward, L.; Whitfield, W.G.; McLennan, A.G.; Safrany, S.T.; Int. J. Biochem. Cell Biol. 42, 1174-1181 (2010)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli M15 cells as a hexahistidine-tagged fusion protein
Drosophila melanogaster
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00007
-
diphosphoinositol pentakisphosphate
in the presence of 10 mM Mg2+, at pH 9.0 and 26°C
Drosophila melanogaster
0.004
-
adenosine tetraphosphate
in the presence of 10 mM Mg2+, at pH 9.0 and 26°C
Drosophila melanogaster
0.011
-
guanosine tetraphosphate
in the presence of 10 mM Mg2+, at pH 9.0 and 26°C
Drosophila melanogaster
0.018
-
diadenosine 5',5'''-P1,P6-hexaphosphate
in the presence of 10 mM Mg2+, at pH 9.0 and 26°C
Drosophila melanogaster
0.13
-
5-phospho-D-ribosyl 1-diphosphate
in the presence of 10 mM Mg2+, at pH 9.0 and 26°C
Drosophila melanogaster
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
Aps is predominantly cytoplasmic
Drosophila melanogaster
5737
-
nucleus
-
Drosophila melanogaster
5634
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
Co2+ supports less than 5% of the activity with Mg2+
Drosophila melanogaster
Mg2+
Mg2+ is the most effective stimulatory cation above 10 mM
Drosophila melanogaster
Mn2+
Mn2+ is also effective, with an optimal concentration of 1 mM
Drosophila melanogaster
additional information
Ca2+, Ni2+ or Zn2+ are ineffective in stimulating activity of Aps
Drosophila melanogaster
Organic Solvent Stability
Organic Solvent
Commentary
Organism
dithiothreitol
in the absence of dithiothreitol a 5 min pretreatment at 36°C lowers enzyme activity by 65%, with temperatures above 56°C virtually abolishing all activity, whereas in the presence of dithiothreitol a 5 min treatment at temperatures up to 76°C is without effect
Drosophila melanogaster
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Drosophila melanogaster
-
-
-
Oxidation Stability
Oxidation Stability
Organism
heat lability is restored by H2O2
Drosophila melanogaster
Purification (Commentary)
Commentary
Organism
Ni-NTA column chromatography
Drosophila melanogaster
Source Tissue
Source Tissue
Commentary
Organism
Textmining
adult
-
Drosophila melanogaster
-
embryo
-
Drosophila melanogaster
-
larva
-
Drosophila melanogaster
-
pupa
-
Drosophila melanogaster
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5-phospho-alpha-D-ribose 1-diphosphate + H2O
-
712171
Drosophila melanogaster
5-phospho-alpha-D-ribose 1-phosphate + phosphate
-
-
-
?
8-oxo-dATP + H2O
low activity
712171
Drosophila melanogaster
?
-
-
-
?
8-oxo-dGTP + H2O
low activity
712171
Drosophila melanogaster
?
-
-
-
?
adenosine tetraphosphate + H2O
the substrate shows great hydrolysis by Aps
712171
Drosophila melanogaster
?
-
-
-
?
ADP-glucose + H2O
low activity
712171
Drosophila melanogaster
?
-
-
-
?
ADP-ribose + H2O
low activity
712171
Drosophila melanogaster
?
-
-
-
?
CTP + H2O
low activity
712171
Drosophila melanogaster
?
-
-
-
?
diadenosine 5',5'''-P1,P5-pentaphosphate + H2O
-
712171
Drosophila melanogaster
?
-
-
-
?
diadenosine 5',5'''-P1,P6-hexaphosphate + H2O
the substrate shows great hydrolysis by Aps
712171
Drosophila melanogaster
?
-
-
-
?
diguanosine pentaphosphate + H2O
low activity
712171
Drosophila melanogaster
?
-
-
-
?
diphosphoinositol pentakisphosphate + H2O
-
712171
Drosophila melanogaster
inositol pentakisphosphate + phosphate
-
-
-
?
diphosphoinositol polyphosphate + H2O
-
712171
Drosophila melanogaster
inositol polyphosphate + phosphate
-
-
-
?
dTTP + H2O
low activity
712171
Drosophila melanogaster
?
-
-
-
?
dUTP + H2O
low activity
712171
Drosophila melanogaster
?
-
-
-
?
GDP-glucose + H2O
low activity
712171
Drosophila melanogaster
?
-
-
-
?
GDP-mannose + H2O
low activity
712171
Drosophila melanogaster
?
-
-
-
?
guanosine tetraphosphate + H2O
the substrate shows the greatest hydrolysis by Aps
712171
Drosophila melanogaster
?
-
-
-
?
additional information
no hydrolysis of diadenosine 5',5'''-P1,P3-polyphosphate, diadenosine 5',5'''-P1,P2-polyphosphate, ATP, ADP, dATP, diguanosine tetraphosphate, diguanosine triphosphate, diguanosine diphosphate, GTP, GDP, dGTP, P1-(5'-adenosyl) P5-(5'-guanosyl) polyphosphate, and P1-(5'-adenosyl) P4-(5'-guanosyl) polyphosphate
712171
Drosophila melanogaster
?
-
-
-
-
TDP-glucose + H2O
low activity
712171
Drosophila melanogaster
?
-
-
-
?
UDP-glucose + H2O
low activity
712171
Drosophila melanogaster
?
-
-
-
?
UDP-N-acetyl-D-galactosamine + H2O
low activity
712171
Drosophila melanogaster
?
-
-
-
?
UDP-N-acetyl-D-glucosamine + H2O
low activity
712171
Drosophila melanogaster
?
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
26
-
at 10 mM Mg2+
Drosophila melanogaster
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.024
-
diphosphoinositol pentakisphosphate
in the presence of 10 mM Mg2+, at pH 9.0 and 26°C
Drosophila melanogaster
0.1
-
5-phospho-D-ribosyl 1-diphosphate
in the presence of 10 mM Mg2+, at pH 9.0 and 26°C
Drosophila melanogaster
0.24
-
adenosine tetraphosphate
in the presence of 10 mM Mg2+, at pH 9.0 and 26°C
Drosophila melanogaster
0.34
-
diadenosine 5',5'''-P1,P6-hexaphosphate
in the presence of 10 mM Mg2+, at pH 9.0 and 26°C
Drosophila melanogaster
0.8
-
guanosine tetraphosphate
in the presence of 10 mM Mg2+, at pH 9.0 and 26°C
Drosophila melanogaster
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
at 10 mM Mg2+
Drosophila melanogaster
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli M15 cells as a hexahistidine-tagged fusion protein
Drosophila melanogaster
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00007
-
diphosphoinositol pentakisphosphate
in the presence of 10 mM Mg2+, at pH 9.0 and 26°C
Drosophila melanogaster
0.004
-
adenosine tetraphosphate
in the presence of 10 mM Mg2+, at pH 9.0 and 26°C
Drosophila melanogaster
0.011
-
guanosine tetraphosphate
in the presence of 10 mM Mg2+, at pH 9.0 and 26°C
Drosophila melanogaster
0.018
-
diadenosine 5',5'''-P1,P6-hexaphosphate
in the presence of 10 mM Mg2+, at pH 9.0 and 26°C
Drosophila melanogaster
0.13
-
5-phospho-D-ribosyl 1-diphosphate
in the presence of 10 mM Mg2+, at pH 9.0 and 26°C
Drosophila melanogaster
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
Aps is predominantly cytoplasmic
Drosophila melanogaster
5737
-
nucleus
-
Drosophila melanogaster
5634
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
Co2+ supports less than 5% of the activity with Mg2+
Drosophila melanogaster
Mg2+
Mg2+ is the most effective stimulatory cation above 10 mM
Drosophila melanogaster
Mn2+
Mn2+ is also effective, with an optimal concentration of 1 mM
Drosophila melanogaster
additional information
Ca2+, Ni2+ or Zn2+ are ineffective in stimulating activity of Aps
Drosophila melanogaster
Organic Solvent Stability (protein specific)
Organic Solvent
Commentary
Organism
dithiothreitol
in the absence of dithiothreitol a 5 min pretreatment at 36°C lowers enzyme activity by 65%, with temperatures above 56°C virtually abolishing all activity, whereas in the presence of dithiothreitol a 5 min treatment at temperatures up to 76°C is without effect
Drosophila melanogaster
Oxidation Stability (protein specific)
Oxidation Stability
Organism
heat lability is restored by H2O2
Drosophila melanogaster
Purification (Commentary) (protein specific)
Commentary
Organism
Ni-NTA column chromatography
Drosophila melanogaster
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
adult
-
Drosophila melanogaster
-
embryo
-
Drosophila melanogaster
-
larva
-
Drosophila melanogaster
-
pupa
-
Drosophila melanogaster
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5-phospho-alpha-D-ribose 1-diphosphate + H2O
-
712171
Drosophila melanogaster
5-phospho-alpha-D-ribose 1-phosphate + phosphate
-
-
-
?
8-oxo-dATP + H2O
low activity
712171
Drosophila melanogaster
?
-
-
-
?
8-oxo-dGTP + H2O
low activity
712171
Drosophila melanogaster
?
-
-
-
?
adenosine tetraphosphate + H2O
the substrate shows great hydrolysis by Aps
712171
Drosophila melanogaster
?
-
-
-
?
ADP-glucose + H2O
low activity
712171
Drosophila melanogaster
?
-
-
-
?
ADP-ribose + H2O
low activity
712171
Drosophila melanogaster
?
-
-
-
?
CTP + H2O
low activity
712171
Drosophila melanogaster
?
-
-
-
?
diadenosine 5',5'''-P1,P5-pentaphosphate + H2O
-
712171
Drosophila melanogaster
?
-
-
-
?
diadenosine 5',5'''-P1,P6-hexaphosphate + H2O
the substrate shows great hydrolysis by Aps
712171
Drosophila melanogaster
?
-
-
-
?
diguanosine pentaphosphate + H2O
low activity
712171
Drosophila melanogaster
?
-
-
-
?
diphosphoinositol pentakisphosphate + H2O
-
712171
Drosophila melanogaster
inositol pentakisphosphate + phosphate
-
-
-
?
diphosphoinositol polyphosphate + H2O
-
712171
Drosophila melanogaster
inositol polyphosphate + phosphate
-
-
-
?
dTTP + H2O
low activity
712171
Drosophila melanogaster
?
-
-
-
?
dUTP + H2O
low activity
712171
Drosophila melanogaster
?
-
-
-
?
GDP-glucose + H2O
low activity
712171
Drosophila melanogaster
?
-
-
-
?
GDP-mannose + H2O
low activity
712171
Drosophila melanogaster
?
-
-
-
?
guanosine tetraphosphate + H2O
the substrate shows the greatest hydrolysis by Aps
712171
Drosophila melanogaster
?
-
-
-
?
additional information
no hydrolysis of diadenosine 5',5'''-P1,P3-polyphosphate, diadenosine 5',5'''-P1,P2-polyphosphate, ATP, ADP, dATP, diguanosine tetraphosphate, diguanosine triphosphate, diguanosine diphosphate, GTP, GDP, dGTP, P1-(5'-adenosyl) P5-(5'-guanosyl) polyphosphate, and P1-(5'-adenosyl) P4-(5'-guanosyl) polyphosphate
712171
Drosophila melanogaster
?
-
-
-
-
TDP-glucose + H2O
low activity
712171
Drosophila melanogaster
?
-
-
-
?
UDP-glucose + H2O
low activity
712171
Drosophila melanogaster
?
-
-
-
?
UDP-N-acetyl-D-galactosamine + H2O
low activity
712171
Drosophila melanogaster
?
-
-
-
?
UDP-N-acetyl-D-glucosamine + H2O
low activity
712171
Drosophila melanogaster
?
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
26
-
at 10 mM Mg2+
Drosophila melanogaster
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.024
-
diphosphoinositol pentakisphosphate
in the presence of 10 mM Mg2+, at pH 9.0 and 26°C
Drosophila melanogaster
0.1
-
5-phospho-D-ribosyl 1-diphosphate
in the presence of 10 mM Mg2+, at pH 9.0 and 26°C
Drosophila melanogaster
0.24
-
adenosine tetraphosphate
in the presence of 10 mM Mg2+, at pH 9.0 and 26°C
Drosophila melanogaster
0.34
-
diadenosine 5',5'''-P1,P6-hexaphosphate
in the presence of 10 mM Mg2+, at pH 9.0 and 26°C
Drosophila melanogaster
0.8
-
guanosine tetraphosphate
in the presence of 10 mM Mg2+, at pH 9.0 and 26°C
Drosophila melanogaster
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
at 10 mM Mg2+
Drosophila melanogaster
Expression
Organism
Commentary
Expression
Drosophila melanogaster
Aps expression is highest in embryos and declines throughout development
down
Expression (protein specific)
Organism
Commentary
Expression
Drosophila melanogaster
Aps expression is highest in embryos and declines throughout development
down
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.8
-
5-phospho-D-ribosyl 1-diphosphate
in the presence of 10 mM Mg2+, at pH 9.0 and 26°C
Drosophila melanogaster
19
-
diadenosine 5',5'''-P1,P6-hexaphosphate
in the presence of 10 mM Mg2+, at pH 9.0 and 26°C
Drosophila melanogaster
60
-
adenosine tetraphosphate
in the presence of 10 mM Mg2+, at pH 9.0 and 26°C
Drosophila melanogaster
71
-
guanosine tetraphosphate
in the presence of 10 mM Mg2+, at pH 9.0 and 26°C
Drosophila melanogaster
342
-
diphosphoinositol pentakisphosphate
in the presence of 10 mM Mg2+, at pH 9.0 and 26°C
Drosophila melanogaster
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.8
-
5-phospho-D-ribosyl 1-diphosphate
in the presence of 10 mM Mg2+, at pH 9.0 and 26°C
Drosophila melanogaster
19
-
diadenosine 5',5'''-P1,P6-hexaphosphate
in the presence of 10 mM Mg2+, at pH 9.0 and 26°C
Drosophila melanogaster
60
-
adenosine tetraphosphate
in the presence of 10 mM Mg2+, at pH 9.0 and 26°C
Drosophila melanogaster
71
-
guanosine tetraphosphate
in the presence of 10 mM Mg2+, at pH 9.0 and 26°C
Drosophila melanogaster
342
-
diphosphoinositol pentakisphosphate
in the presence of 10 mM Mg2+, at pH 9.0 and 26°C
Drosophila melanogaster
Other publictions for EC 3.6.1.52
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733091
Wu
Elucidating diphosphoinositol ...
Homo sapiens
Angew. Chem. Int. Ed. Engl.
53
7192-7197
2014
-
-
-
-
-
-
3
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
719961
Lonetti
Identification of an evolution ...
Homo sapiens
J. Biol. Chem.
286
31966-31974
2011
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
712171
Winward
Oxidation of the diphosphoinos ...
Drosophila melanogaster
Int. J. Biochem. Cell Biol.
42
1174-1181
2010
-
-
1
-
-
-
-
5
2
4
-
-
1
2
1
-
1
-
-
4
-
-
22
-
1
-
-
5
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
5
2
4
-
-
1
1
-
1
-
4
-
-
22
-
1
-
-
5
1
-
-
-
1
-
-
1
5
5
668226
Chu
Ectopic expression of murine d ...
Mus musculus
Cell. Signal.
16
1045-1059
2004
-
-
1
-
-
-
-
1
-
-
1
-
-
3
-
-
1
-
-
7
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
1
-
7
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
657829
Hua
Paralogous murine Nudt10 and N ...
Mus musculus
Biochem. J.
373
81-89
2003
-
-
1
-
-
-
-
-
-
-
2
1
-
5
-
1
1
-
-
1
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
1
-
-
1
1
-
1
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
659184
Hidaka
An adjacent pair of human NUDT ...
Homo sapiens
J. Biol. Chem.
277
32730-32738
2002
-
-
1
-
1
-
-
-
1
-
1
2
-
3
-
1
1
-
-
2
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
-
2
2
-
-
2
2
-
3
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
659817
Cartwright
The g5R (D250) gene of African ...
African swine fever virus
J. Virol.
76
1415-1421
2002
-
-
1
-
-
-
-
1
-
2
1
1
-
2
-
1
1
-
-
1
-
-
1
-
-
-
-
-
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
2
1
1
-
-
1
1
-
1
-
-
1
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
393827
Caffrey
Discovery of molecular and cat ...
Homo sapiens
J. Biol. Chem.
275
12730-12736
2000
-
-
1
-
-
-
-
2
-
-
1
-
-
4
-
-
-
-
-
-
-
-
1
1
-
-
-
3
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
3
-
-
-
-
-
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-
393825
Safrany
The diadenosine hexaphosphate ...
Homo sapiens
J. Biol. Chem.
274
21735-21740
1999
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-
-
-
-
-
-
2
-
-
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5
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-
-
-
-
-
-
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3
-
-
-
-
4
-
-
-
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-
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-
-
-
-
2
-
-
-
-
-
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-
-
-
-
-
-
3
-
-
-
-
4
-
-
-
-
-
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-
-
-
393826
Yang
Site-directed mutagenesis of d ...
Homo sapiens
J. Biol. Chem.
274
35434-35440
1999
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-
-
-
10
-
-
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-
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-
-
-
2
-
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-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
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-
-
-
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-
393824
Safrany
A novel context for the 'MutT' ...
Homo sapiens, Rattus norvegicus
EMBO J.
17
6599-6607
1998
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1
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2
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2
2
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1
3
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4
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1
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2
1
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8
1
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-
-
2
1
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2
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1
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2
-
-
2
2
2
-
1
3
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1
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2
1
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8
1
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2
1
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