BRENDA - Enzyme Database show
show all sequences of 3.6.1.52

A novel context for the 'MutT' module, a guardian of cell integrity, in a diphosphoinositol polyphosphate phosphohydrolase

Safrany, S.T.; Caffrey, J.J.; Yang, X.; Bembenek, M.E.; Moyer, M.B.; Burkhart, W.A.; Shears, S.B.; EMBO J. 17, 6599-6607 (1998)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Homo sapiens
Engineering
Amino acid exchange
Commentary
Organism
E70Q
-
Homo sapiens
E70Q
greatly reduced rate of hydrolysis of diphosphoinositol pentakisphosphate or bisdiphosphoinositol tetrakisphosphate
Rattus norvegicus
Inhibitors
Inhibitors
Commentary
Organism
Structure
F-
uncompetitive against bisdiphosphoinositol tetrakisphosphate, non-competitive against diphosphoinositol pentakisphosphate
Rattus norvegicus
Inositol hexakisphosphate
-
Rattus norvegicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.000034
-
bisdiphosphoinositol tetrakisphosphate
-
Rattus norvegicus
0.00034
-
diphosphoinositol pentakisphosphate
-
Rattus norvegicus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
1-2 mM required
Rattus norvegicus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
18000
-
gel filtration
Rattus norvegicus
19500
-
x * 19500, calculation from nucleotide sequence
Homo sapiens
25000
-
x * 25000, recombinant enzyme expressed in Escherichia coli, SDS-PAGE
Homo sapiens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
recombinant enzyme
-
Rattus norvegicus
-
-
-
Purification (Commentary)
Commentary
Organism
-
Rattus norvegicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Rattus norvegicus
-
uterus
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Rattus norvegicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
bisdiphosphoinositol tetrakisphosphate + H2O
-
393824
Homo sapiens
? + phosphate
-
-
-
?
bisdiphosphoinositol tetrakisphosphate + H2O
-
393824
Rattus norvegicus
? + phosphate
-
-
-
?
dATP + H2O
-
393824
Homo sapiens
dADP + phosphate
-
393824
Homo sapiens
-
diphosphoinositol pentakisphosphate + H2O
-
393824
Rattus norvegicus
inositol pentakisphosphate + phosphate
-
-
-
?
diphosphoinositol pentakisphosphate + H2O
the diphosphate group that is attached to the 5-carbon is hydrolyzed, no release of diphosphate
393824
Homo sapiens
inositol pentakisphosphate + phosphate
-
-
-
?
additional information
no activity with ATP
393824
Rattus norvegicus
?
-
-
-
-
additional information
the MuT domain of the enzyme participates in the hydrolysis of dATP, diphosphoinositol pentakisphosphate or bisdiphosphoinositol tetrakisphosphate
393824
Homo sapiens
?
-
-
-
-
additional information
the MuT domain of the enzyme participates in the hydrolysis of dATP, diphosphoinositol pentakisphosphate or bisdiphosphoinositol tetrakisphosphate
393824
Rattus norvegicus
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 19500, calculation from nucleotide sequence; x * 25000, recombinant enzyme expressed in Escherichia coli, SDS-PAGE
Homo sapiens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
7.5
hydrolysis of diphosphoinositol pentakisphosphate
Homo sapiens
9
-
hydrolysis of dATP
Homo sapiens
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.5
8
pH 6.5: about 55% of maximal activity, pH 8.0: about 65% of maximal activity, hydrolysis of diphosphoinositol pentakisphosphate
Homo sapiens
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0084
-
F-
against bisdiphosphoinositol tetrakisphosphate
Rattus norvegicus
0.0109
-
F-
against diphosphoinositol pentakisphosphate
Rattus norvegicus
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Homo sapiens
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
E70Q
-
Homo sapiens
E70Q
greatly reduced rate of hydrolysis of diphosphoinositol pentakisphosphate or bisdiphosphoinositol tetrakisphosphate
Rattus norvegicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
F-
uncompetitive against bisdiphosphoinositol tetrakisphosphate, non-competitive against diphosphoinositol pentakisphosphate
Rattus norvegicus
Inositol hexakisphosphate
-
Rattus norvegicus
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0084
-
F-
against bisdiphosphoinositol tetrakisphosphate
Rattus norvegicus
0.0109
-
F-
against diphosphoinositol pentakisphosphate
Rattus norvegicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.000034
-
bisdiphosphoinositol tetrakisphosphate
-
Rattus norvegicus
0.00034
-
diphosphoinositol pentakisphosphate
-
Rattus norvegicus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
1-2 mM required
Rattus norvegicus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
18000
-
gel filtration
Rattus norvegicus
19500
-
x * 19500, calculation from nucleotide sequence
Homo sapiens
25000
-
x * 25000, recombinant enzyme expressed in Escherichia coli, SDS-PAGE
Homo sapiens
Purification (Commentary) (protein specific)
Commentary
Organism
-
Rattus norvegicus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Rattus norvegicus
-
uterus
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
bisdiphosphoinositol tetrakisphosphate + H2O
-
393824
Homo sapiens
? + phosphate
-
-
-
?
bisdiphosphoinositol tetrakisphosphate + H2O
-
393824
Rattus norvegicus
? + phosphate
-
-
-
?
dATP + H2O
-
393824
Homo sapiens
dADP + phosphate
-
393824
Homo sapiens
-
diphosphoinositol pentakisphosphate + H2O
-
393824
Rattus norvegicus
inositol pentakisphosphate + phosphate
-
-
-
?
diphosphoinositol pentakisphosphate + H2O
the diphosphate group that is attached to the 5-carbon is hydrolyzed, no release of diphosphate
393824
Homo sapiens
inositol pentakisphosphate + phosphate
-
-
-
?
additional information
no activity with ATP
393824
Rattus norvegicus
?
-
-
-
-
additional information
the MuT domain of the enzyme participates in the hydrolysis of dATP, diphosphoinositol pentakisphosphate or bisdiphosphoinositol tetrakisphosphate
393824
Homo sapiens
?
-
-
-
-
additional information
the MuT domain of the enzyme participates in the hydrolysis of dATP, diphosphoinositol pentakisphosphate or bisdiphosphoinositol tetrakisphosphate
393824
Rattus norvegicus
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 19500, calculation from nucleotide sequence; x * 25000, recombinant enzyme expressed in Escherichia coli, SDS-PAGE
Homo sapiens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
7.5
hydrolysis of diphosphoinositol pentakisphosphate
Homo sapiens
9
-
hydrolysis of dATP
Homo sapiens
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.5
8
pH 6.5: about 55% of maximal activity, pH 8.0: about 65% of maximal activity, hydrolysis of diphosphoinositol pentakisphosphate
Homo sapiens
Other publictions for EC 3.6.1.52
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733091
Wu
Elucidating diphosphoinositol ...
Homo sapiens
Angew. Chem. Int. Ed. Engl.
53
7192-7197
2014
-
-
-
-
-
-
3
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
719961
Lonetti
Identification of an evolution ...
Homo sapiens
J. Biol. Chem.
286
31966-31974
2011
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
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-
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-
-
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-
1
-
-
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-
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-
712171
Winward
Oxidation of the diphosphoinos ...
Drosophila melanogaster
Int. J. Biochem. Cell Biol.
42
1174-1181
2010
-
-
1
-
-
-
-
5
2
4
-
-
1
2
1
-
1
-
-
4
-
-
22
-
1
-
-
5
1
-
-
-
-
-
-
-
-
1
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-
-
-
-
-
-
5
2
4
-
-
1
1
-
1
-
4
-
-
22
-
1
-
-
5
1
-
-
-
1
-
-
1
5
5
668226
Chu
Ectopic expression of murine d ...
Mus musculus
Cell. Signal.
16
1045-1059
2004
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-
1
-
-
-
-
1
-
-
1
-
-
3
-
-
1
-
-
7
-
-
1
-
-
-
-
-
-
-
-
-
-
-
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-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
1
-
7
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
657829
Hua
Paralogous murine Nudt10 and N ...
Mus musculus
Biochem. J.
373
81-89
2003
-
-
1
-
-
-
-
-
-
-
2
1
-
5
-
1
1
-
-
1
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
1
-
-
1
1
-
1
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
659184
Hidaka
An adjacent pair of human NUDT ...
Homo sapiens
J. Biol. Chem.
277
32730-32738
2002
-
-
1
-
1
-
-
-
1
-
1
2
-
3
-
1
1
-
-
2
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
-
2
2
-
-
2
2
-
3
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
659817
Cartwright
The g5R (D250) gene of African ...
African swine fever virus
J. Virol.
76
1415-1421
2002
-
-
1
-
-
-
-
1
-
2
1
1
-
2
-
1
1
-
-
1
-
-
1
-
-
-
-
-
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
2
1
1
-
-
1
1
-
1
-
-
1
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
393827
Caffrey
Discovery of molecular and cat ...
Homo sapiens
J. Biol. Chem.
275
12730-12736
2000
-
-
1
-
-
-
-
2
-
-
1
-
-
4
-
-
-
-
-
-
-
-
1
1
-
-
-
3
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
3
-
-
-
-
-
-
-
-
-
-
393825
Safrany
The diadenosine hexaphosphate ...
Homo sapiens
J. Biol. Chem.
274
21735-21740
1999
-
-
-
-
-
-
-
2
-
-
-
-
-
5
-
-
-
-
-
-
-
-
3
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
393826
Yang
Site-directed mutagenesis of d ...
Homo sapiens
J. Biol. Chem.
274
35434-35440
1999
-
-
-
-
10
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
3
-
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-
-
-
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-
-
10
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
393824
Safrany
A novel context for the 'MutT' ...
Homo sapiens, Rattus norvegicus
EMBO J.
17
6599-6607
1998
-
-
1
-
2
-
2
2
-
1
3
-
-
4
-
-
1
-
-
2
1
-
8
1
-
-
-
-
2
1
-
-
2
-
-
-
-
1
-
-
2
-
-
2
2
2
-
1
3
-
-
-
-
1
-
2
1
-
8
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-