Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
E70Q | - |
Homo sapiens |
E70Q | greatly reduced rate of hydrolysis of diphosphoinositol pentakisphosphate or bisdiphosphoinositol tetrakisphosphate | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
F- | uncompetitive against bisdiphosphoinositol tetrakisphosphate, non-competitive against diphosphoinositol pentakisphosphate | Rattus norvegicus | |
Inositol hexakisphosphate | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000034 | - |
bisdiphosphoinositol tetrakisphosphate | - |
Rattus norvegicus | |
0.00034 | - |
diphosphoinositol pentakisphosphate | - |
Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 1-2 mM required | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18000 | - |
gel filtration | Rattus norvegicus |
19500 | - |
x * 19500, calculation from nucleotide sequence | Homo sapiens |
25000 | - |
x * 25000, recombinant enzyme expressed in Escherichia coli, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
recombinant enzyme | - |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
uterus | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
bisdiphosphoinositol tetrakisphosphate + H2O | - |
Homo sapiens | ? + phosphate | - |
? | |
bisdiphosphoinositol tetrakisphosphate + H2O | - |
Rattus norvegicus | ? + phosphate | - |
? | |
dATP + H2O | - |
Homo sapiens | dADP + phosphate | - |
? | |
diphosphoinositol pentakisphosphate + H2O | - |
Rattus norvegicus | inositol hexakisphosphate + phosphate | - |
? | |
diphosphoinositol pentakisphosphate + H2O | the diphosphate group that is attached to the 5-carbon is hydrolyzed, no release of diphosphate | Homo sapiens | inositol hexakisphosphate + phosphate | - |
? | |
additional information | no activity with ATP | Rattus norvegicus | ? | - |
? | |
additional information | the MuT domain of the enzyme participates in the hydrolysis of dATP, diphosphoinositol pentakisphosphate or bisdiphosphoinositol tetrakisphosphate | Homo sapiens | ? | - |
? | |
additional information | the MuT domain of the enzyme participates in the hydrolysis of dATP, diphosphoinositol pentakisphosphate or bisdiphosphoinositol tetrakisphosphate | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 19500, calculation from nucleotide sequence | Homo sapiens |
? | x * 25000, recombinant enzyme expressed in Escherichia coli, SDS-PAGE | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | hydrolysis of diphosphoinositol pentakisphosphate | Homo sapiens |
9 | - |
hydrolysis of dATP | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8 | pH 6.5: about 55% of maximal activity, pH 8.0: about 65% of maximal activity, hydrolysis of diphosphoinositol pentakisphosphate | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0084 | - |
F- | against bisdiphosphoinositol tetrakisphosphate | Rattus norvegicus | |
0.0109 | - |
F- | against diphosphoinositol pentakisphosphate | Rattus norvegicus |