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Literature summary for 3.6.1.52 extracted from

  • Safrany, S.T.; Caffrey, J.J.; Yang, X.; Bembenek, M.E.; Moyer, M.B.; Burkhart, W.A.; Shears, S.B.
    A novel context for the 'MutT' module, a guardian of cell integrity, in a diphosphoinositol polyphosphate phosphohydrolase (1998), EMBO J., 17, 6599-6607.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
E70Q
-
Homo sapiens
E70Q greatly reduced rate of hydrolysis of diphosphoinositol pentakisphosphate or bisdiphosphoinositol tetrakisphosphate Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
F- uncompetitive against bisdiphosphoinositol tetrakisphosphate, non-competitive against diphosphoinositol pentakisphosphate Rattus norvegicus
Inositol hexakisphosphate
-
Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000034
-
bisdiphosphoinositol tetrakisphosphate
-
Rattus norvegicus
0.00034
-
diphosphoinositol pentakisphosphate
-
Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 1-2 mM required Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18000
-
gel filtration Rattus norvegicus
19500
-
x * 19500, calculation from nucleotide sequence Homo sapiens
25000
-
x * 25000, recombinant enzyme expressed in Escherichia coli, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
recombinant enzyme
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-
uterus
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
bisdiphosphoinositol tetrakisphosphate + H2O
-
Homo sapiens ? + phosphate
-
?
bisdiphosphoinositol tetrakisphosphate + H2O
-
Rattus norvegicus ? + phosphate
-
?
dATP + H2O
-
Homo sapiens dADP + phosphate
-
?
diphosphoinositol pentakisphosphate + H2O
-
Rattus norvegicus inositol hexakisphosphate + phosphate
-
?
diphosphoinositol pentakisphosphate + H2O the diphosphate group that is attached to the 5-carbon is hydrolyzed, no release of diphosphate Homo sapiens inositol hexakisphosphate + phosphate
-
?
additional information no activity with ATP Rattus norvegicus ?
-
?
additional information the MuT domain of the enzyme participates in the hydrolysis of dATP, diphosphoinositol pentakisphosphate or bisdiphosphoinositol tetrakisphosphate Homo sapiens ?
-
?
additional information the MuT domain of the enzyme participates in the hydrolysis of dATP, diphosphoinositol pentakisphosphate or bisdiphosphoinositol tetrakisphosphate Rattus norvegicus ?
-
?

Subunits

Subunits Comment Organism
? x * 19500, calculation from nucleotide sequence Homo sapiens
? x * 25000, recombinant enzyme expressed in Escherichia coli, SDS-PAGE Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5 hydrolysis of diphosphoinositol pentakisphosphate Homo sapiens
9
-
hydrolysis of dATP Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8 pH 6.5: about 55% of maximal activity, pH 8.0: about 65% of maximal activity, hydrolysis of diphosphoinositol pentakisphosphate Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0084
-
F- against bisdiphosphoinositol tetrakisphosphate Rattus norvegicus
0.0109
-
F- against diphosphoinositol pentakisphosphate Rattus norvegicus