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Literature summary for 3.6.1.5 extracted from

  • Wujak, M.; Banach, M.; Porowinska, D.; Piskulak, K.; Komoszynski, M.
    Isolation and bioinformatic analysis of seven genes encoding potato apyrase. Bacterial overexpresssion, refolding and initial kinetic studies on some recombinant potato apyrases (2013), Phytochemistry, 93, 8-17.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
isoforms APY4, APY5 and APY6 are expressed in Escherichia coli BL21(DE3) cells with thioredoxin-tag, His6-tag and S-tag Solanum tuberosum

General Stability

General Stability Organism
the removal of thioredoxin-tag from purified apyrase causes 10times reduction of specific activity Solanum tuberosum

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
Solanum tuberosum
EDTA complete inhibition at 10 mM Solanum tuberosum

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ best activator of isoform APY6 Solanum tuberosum
Co2+ activator of isoform APY6 Solanum tuberosum
Mg2+ an increase in ADPase activity in presence of Mg2+ ions occurs in case of isoform APY5 Solanum tuberosum
Mn2+ activator of isoform APY6 Solanum tuberosum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36989
-
x * 36989, isoform APY6, calculated from amino acid sequence Solanum tuberosum
48748
-
x * 48748, isoform APY5, calculated from amino acid sequence Solanum tuberosum
49765
-
x * 49765, isoform APY4, calculated from amino acid sequence Solanum tuberosum
50004
-
x * 50004, isoform APY9, calculated from amino acid sequence Solanum tuberosum
50032
-
x * 50032, isoform APY8, calculated from amino acid sequence Solanum tuberosum
50062
-
x * 50062, isoform APY10, calculated from amino acid sequence Solanum tuberosum
50077
-
x * 50077, isoform APY7, calculated from amino acid sequence Solanum tuberosum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ADP + H2O Solanum tuberosum 68% activity compared to ATP AMP + phosphate
-
?
ATP + 2 H2O Solanum tuberosum 100% activity AMP + 2 phosphate
-
?

Organism

Organism UniProt Comment Textmining
Solanum tuberosum
-
variety Saturna
-

Purification (Commentary)

Purification (Comment) Organism
isoform APY6, Superdex 75 gel filtration Solanum tuberosum

Renatured (Commentary)

Renatured (Comment) Organism
isoform APY6 in 50 mM Tris-HCl pH 6.5 buffer containing 6 M guanidine hydrochloride and 10 mM 2-mercaptoethanol Solanum tuberosum

Source Tissue

Source Tissue Comment Organism Textmining
tuber
-
Solanum tuberosum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + H2O 68% activity compared to ATP Solanum tuberosum AMP + phosphate
-
?
ATP + 2 H2O 100% activity Solanum tuberosum AMP + 2 phosphate
-
?
CDP + H2O 3.34% activity compared to ATP Solanum tuberosum CMP + phosphate
-
?
CTP + 2 H2O 12.5% activity compared to ATP Solanum tuberosum CMP + 2 phosphate
-
?
GDP + H2O 69.2% activity compared to ATP Solanum tuberosum GMP + phosphate
-
?
GTP + 2 H2O 84.6% activity compared to ATP Solanum tuberosum GMP + 2 phosphate
-
?
UDP + H2O 6.8% activity compared to ATP Solanum tuberosum UMP + phosphate
-
?
UTP + 2 H2O 28.2% activity compared to ATP Solanum tuberosum UMP + 2 phosphate
-
?

Subunits

Subunits Comment Organism
? x * 36989, isoform APY6, calculated from amino acid sequence Solanum tuberosum
? x * 48748, isoform APY5, calculated from amino acid sequence Solanum tuberosum
? x * 49765, isoform APY4, calculated from amino acid sequence Solanum tuberosum
? x * 50004, isoform APY9, calculated from amino acid sequence Solanum tuberosum
? x * 50032, isoform APY8, calculated from amino acid sequence Solanum tuberosum
? x * 50062, isoform APY10, calculated from amino acid sequence Solanum tuberosum
? x * 50077, isoform APY7, calculated from amino acid sequence Solanum tuberosum

Synonyms

Synonyms Comment Organism
APY10 isoform Solanum tuberosum
APY4 isoform Solanum tuberosum
APY5 isoform Solanum tuberosum
APY6 isoform Solanum tuberosum
APY7 isoform Solanum tuberosum
APY8 isoform Solanum tuberosum
APY9 isoform Solanum tuberosum
NTPDase
-
Solanum tuberosum
nucleoside triphosphate diphosphohydrolase
-
Solanum tuberosum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
isoform APY6 Solanum tuberosum

pI Value

Organism Comment pI Value Maximum pI Value
Solanum tuberosum isoform APY4, calculated from amino acid sequence
-
6.6
Solanum tuberosum isoform APY7, calculated from amino acid sequence
-
6.6
Solanum tuberosum isoform APY8, calculated from amino acid sequence
-
6.6
Solanum tuberosum isoform APY9, calculated from amino acid sequence
-
6.6
Solanum tuberosum isoform APY10, calculated from amino acid sequence
-
7.2
Solanum tuberosum isoform APY5, calculated from amino acid sequence
-
7.2
Solanum tuberosum isoform APY6, calculated from amino acid sequence
-
8.4