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Literature summary for 3.6.1.5 extracted from

  • Manque, P.A.; Woehlbier, U.; Lara, A.M.; Tenjo, F.; Alves, J.M.; Buck, G.A.
    Identification and characterization of a novel calcium-activated apyrase from Cryptosporidium parasites and its potential role in pathogenesis (2012), PLoS ONE, 7, e31030.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.1.5

Cloned(Commentary)

Cloned (Comment) Organism
gene capy, single copy gene, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of CApy C-terminally fused to a His6/S-tag in Escherichia coli strain BL21(D3) Cryptosporidium parvum

Inhibitors

Inhibitors Comment Organism Structure
additional information antibodies directed against CApy block Cryptosporidium parvum sporozoite invasion of HCT-8 cells Cryptosporidium parvum

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Cryptosporidium parvum
Mg2+ required Cryptosporidium parvum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36932
-
 x * 36932, sequence calculation, x * 50000, about, recombinant enzyme, SDS-PAGE Cryptosporidium parvum
50000
-
 x * 36932, sequence calculation, x * 50000, about, recombinant enzyme, SDS-PAGE Cryptosporidium parvum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ADP + H2O Cryptosporidium parvum
-
 AMP + phosphate
-
 ?
ATP + 2 H2O Cryptosporidium parvum
-
 AMP + 2 phosphate
-
 ?
GDP + H2O Cryptosporidium parvum
-
 GMP + phosphate
-
 ?
UDP + H2O Cryptosporidium parvum
-
 UMP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Cryptosporidium parvum Q5CXD0 gene capy
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein CApy in its mature form is posttranslationally glycosylated in an N-linked fashion Cryptosporidium parvum

Purification (Commentary)

Purification (Comment) Organism
recombinant rCApy C-terminally fused to a His6/S-tag solubilized from inclusion bodies after expression in Escherichia coli strain BL21(DE3), by nickel and glutathione affinity chromatography Cryptosporidium parvum

Renatured (Commentary)

Renatured (Comment) Organism
recombinant rCApy from inclusion bodies after expression in Escherichia coli strain BL21(DE3), resuspended in 20 mM sodium phosphate, pH 7.4, 500 mM NaCl, 6 M guanidine hydrochloride Cryptosporidium parvum

Source Tissue

Source Tissue Comment Organism Textmining
additional information isolated CApy interacts with the host cell Cryptosporidium parvum
-
oocyst
-
 Cryptosporidium parvum
-
sporozoite polar localization of Capy, possible co-localization with the apical complex of the parasite Cryptosporidium parvum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + H2O
-
 Cryptosporidium parvum AMP + phosphate
-
 ?
ATP + 2 H2O
-
 Cryptosporidium parvum AMP + 2 phosphate
-
 ?
GDP + H2O
-
 Cryptosporidium parvum GMP + phosphate
-
 ?
additional information UDP and GDP, rather than ADP or ATP, are the preferred substrates of CApy Cryptosporidium parvum ?
-
 ?
UDP + H2O
-
 Cryptosporidium parvum UMP + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 36932, sequence calculation, x * 50000, about, recombinant enzyme, SDS-PAGE Cryptosporidium parvum

Synonyms

Synonyms Comment Organism
Ca2+-activated nucleoside diphosphatase
-
 Cryptosporidium parvum
CApy
-
 Cryptosporidium parvum
ecto-ATP diphosphohydrolase
-
 Cryptosporidium parvum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
23
-
 assay at Cryptosporidium parvum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Cryptosporidium parvum

pI Value

Organism Comment pI Value Maximum pI Value
Cryptosporidium parvum sequence calculation
-
 5.23

General Information

General Information Comment Organism
evolution the capy gene is most likely an ancestral feature that has been lost from most apicomplexan genomes except Cryptosporidium, Neospora and Toxoplasma Cryptosporidium parvum
physiological function antibodies directed against CApy block Cryptosporidium parvum sporozoite invasion of HCT-8 cells Cryptosporidium parvum