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Literature summary for 3.6.1.5 extracted from
- Cloning, expression, and characterization of salivary apyrase from Aedes albopictus (2012), Parasitol. Res., 110, 931-937.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of His-tagged enzyme in Escherichia coli | Aedes albopictus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | required | Aedes albopictus |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 2 H2O | Aedes albopictus | - |
AMP + 2 phosphate | - |
? | |
| additional information | Aedes albopictus | apyrases hydrolyze the phosphodiester bonds of nucleoside tri- and diphosphates to orthophosphate and mononucleodides | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aedes albopictus | E0D877 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography, denaturing by urea and renaturing using glutathione and dialysis | Aedes albopictus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| salivary gland | - |
Aedes albopictus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 2 H2O | - |
Aedes albopictus | AMP + 2 phosphate | - |
? | |
| additional information | apyrases hydrolyze the phosphodiester bonds of nucleoside tri- and diphosphates to orthophosphate and mononucleodides | Aedes albopictus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ATP diphosphohydrolase | - |
Aedes albopictus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Aedes albopictus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Aedes albopictus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | apyrases can inhibit platelet activation by depletion of adenosine diphosphate | Aedes albopictus |
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