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Literature summary for 3.6.1.5 extracted from

  • Leite, M.d.S.; Thomaz, R.; Fonseca, F.V.; Panizzutti, R.; Vercesi, A.E.; Meyer-Fernandes, J.R.
    Trypanosoma brucei brucei: Biochemical characterization of ecto-nucleoside triphosphate diphosphohydrolase activities (2007), Exp. Parasitol., 115, 315-323.
    View publication on PubMed
Search for more literature for 3.6.1.5

Activating Compound

Activating Compound Comment Organism Structure
bafilomycin 13% activation at 0.001 mM Trypanosoma brucei brucei
Ouabain 13% activation at 1 mM Trypanosoma brucei brucei
Tartrate 14% activation at 10 mM Trypanosoma brucei brucei

Inhibitors

Inhibitors Comment Organism Structure
4,4'-diisothiocyanostilbene 2,2'-disulfonate DIDS, an impermeable inhibitor Trypanosoma brucei brucei
4-nitrophenyl phosphate 15% inhibition at 5 mM Trypanosoma brucei brucei
5'-AMP 11% inhibition at 5 mM Trypanosoma brucei brucei
ADP 50% inhibition at 5 mM Trypanosoma brucei brucei
dipyridamole 10% inhibition at 0.01 mM Trypanosoma brucei brucei
Furosemide 20% inhibition at 1 mM Trypanosoma brucei brucei
additional information no inhibition by oligomycin, ouabain, and molybdate Trypanosoma brucei brucei
NaN3 58% inhibition at 10 mM Trypanosoma brucei brucei
suramin a P2 purinoreceptor antagonist, 50% inhibition at 0.05-0.5 mM Trypanosoma brucei brucei
vanadate 15% inhibition at 1 mM Trypanosoma brucei brucei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.61
-
 ATP pH 7.2, 30°C Trypanosoma brucei brucei

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates Trypanosoma brucei brucei
Mg2+ activates, most effect divalent cation, activity profile, overview Trypanosoma brucei brucei
Mn2+ activates, most effect divalent cation Trypanosoma brucei brucei
Zn2+ activates Trypanosoma brucei brucei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ADP + H2O Trypanosoma brucei brucei hydrolysis of extracellular ADP AMP + phosphate
-
 ?
AMP + H2O Trypanosoma brucei brucei hydrolysis of extracellular AMP, low activity adenosine + phosphate
-
 ?
ATP + H2O Trypanosoma brucei brucei hydrolysis of extracellular ATP ADP + phosphate
-
 ?
additional information Trypanosoma brucei brucei the enzyme plays a role in the salvage of purines from the extracellular medium in the organism ?
-
 ?

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei brucei
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
integument
-
 Trypanosoma brucei brucei
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Trypanosoma brucei brucei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + H2O
-
 Trypanosoma brucei brucei AMP + phosphate
-
 ?
ADP + H2O hydrolysis of extracellular ADP Trypanosoma brucei brucei AMP + phosphate
-
 ?
AMP + H2O low activity Trypanosoma brucei brucei adenosine + phosphate
-
 ?
AMP + H2O hydrolysis of extracellular AMP, low activity Trypanosoma brucei brucei adenosine + phosphate
-
 ?
ATP + H2O
-
 Trypanosoma brucei brucei ADP + phosphate
-
 ?
ATP + H2O hydrolysis of extracellular ATP Trypanosoma brucei brucei ADP + phosphate
-
 ?
CTP + H2O
-
 Trypanosoma brucei brucei CDP + phosphate
-
 ?
GTP + H2O
-
 Trypanosoma brucei brucei GDP + phosphate
-
 ?
ITP + H2O best substrate Trypanosoma brucei brucei IDP + phosphate
-
 ?
additional information substrate specificity, overview Trypanosoma brucei brucei ?
-
 ?
additional information the enzyme plays a role in the salvage of purines from the extracellular medium in the organism Trypanosoma brucei brucei ?
-
 ?
TTP + H2O low activity Trypanosoma brucei brucei TDP + phosphate
-
 ?
UTP + H2O
-
 Trypanosoma brucei brucei UDP + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
E-NTPDase
-
 Trypanosoma brucei brucei
ecto-ATPase
-
 Trypanosoma brucei brucei
ecto-nucleoside triphosphate diphosphohydrolase
-
 Trypanosoma brucei brucei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Trypanosoma brucei brucei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Trypanosoma brucei brucei