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Literature summary for 3.6.1.5 extracted from

  • Xu, W.; Jones, C.R.; Dunn, C.A.; Bessman, M.J.
    Gene ytkD of Bacillus subtilis encodes an atypical nucleoside triphosphatase member of the Nudix hydrolase superfamily (2004), J. Bacteriol., 186, 8380-8384.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.1.5

Cloned(Commentary)

Cloned (Comment) Organism
Bacillus subtilis gene ytkD cloned and expressed in Escherichia coli Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.16
-
 dGTP pH 8.5, 37°C Bacillus subtilis
0.43
-
 8-oxo-dGTP pH 8.5, 37°C Bacillus subtilis
0.89
-
 dATP pH 8.5, 37°C Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Bacillus subtilis
Mn2+ 15% residual activity compared with Mg2+ Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
8-oxo-dGTP + 2 H2O
-
 Bacillus subtilis 8-oxo-dGMP + 2 phosphate
-
 ?
adenosine (5')-tetraphospho-(5')-adenosine + H2O
-
 Bacillus subtilis ?
-
 ?
ADP-ribose + H2O
-
 Bacillus subtilis ?
-
 ?
ATP + 2 H2O
-
 Bacillus subtilis AMP + 2 phosphate
-
 ?
CDP-choline + H2O
-
 Bacillus subtilis ?
-
 ?
CDP-choline + H2O
-
 Bacillus subtilis 168 ?
-
 ?
CTP + 2 H2O
-
 Bacillus subtilis CMP + 2 phosphate
-
 ?
dATP + H2O
-
 Bacillus subtilis dAMP + phosphate + H+
-
 ?
dCTP + H2O
-
 Bacillus subtilis dCMP + phosphate + H+
-
 ?
dGTP + H2O
-
 Bacillus subtilis dGMP + phosphate + H+
-
 ?
dTTP + H2O
-
 Bacillus subtilis dTMP + phosphate + H+
-
 ?
dUTP + H2O
-
 Bacillus subtilis dUMP + phosphate + H+
-
 ?
FAD + H2O
-
 Bacillus subtilis ?
-
 ?
FAD + H2O
-
 Bacillus subtilis 168 ?
-
 ?
GDP-mannose + H2O
-
 Bacillus subtilis ?
-
 ?
GDP-mannose + H2O
-
 Bacillus subtilis 168 ?
-
 ?
GTP + 2 H2O
-
 Bacillus subtilis GMP + 2 phosphate
-
 ?
NADH + H2O
-
 Bacillus subtilis ?
-
 ?
UDP-glucose + H2O
-
 Bacillus subtilis ?
-
 ?
UTP + 2 H2O
-
 Bacillus subtilis UMP + 2 phosphate
-
 ?
UTP + 2 H2O
-
 Bacillus subtilis 168 UMP + 2 phosphate
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.74
-
 dGTP pH 8.5, 37°C Bacillus subtilis
0.96
-
 8-oxo-dGTP pH 8.5, 37°C Bacillus subtilis
1.5
-
 dATP pH 8.5, 37°C Bacillus subtilis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.6
-
 dATP pH 8.5, 37°C Bacillus subtilis
2.4
-
 8-oxo-dGTP pH 8.5, 37°C Bacillus subtilis
4.6
-
 dGTP pH 8.5, 37°C Bacillus subtilis