Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NP-40 | 0.1%, about 3fold activation | Gallus gallus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
azide | at 10 mM and pH 7.4, 21% inhibition of ATP hydrolysis, 85% inhibition of ADP hydrolysis | Gallus gallus | |
diphosphate | at 10 mM and pH 7.4, 51% inhibition of ATP hydrolysis, 97% inhibition of ADP hydrolysis | Gallus gallus | |
fluoride | at 10 mM and pH 7.4, 2% inhibition of ATP hydrolysis, 91% inhibition of ADP hydrolysis | Gallus gallus | |
additional information | not inhibitory: Concanavalin A, suramin | Gallus gallus | |
vanadate | at 10 mM and pH 7.4, 7% inhibition of ATP hydrolysis, 54% inhibition of ADP hydrolysis | Gallus gallus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
ATP | pH 6.4, 37°C | Gallus gallus | |
0.51 | - |
ATP | pH 7.4, 37°C | Gallus gallus | |
0.72 | - |
ADP | pH 6.4, 37°C | Gallus gallus | |
5.3 | - |
ADP | pH 7.4, 37°C | Gallus gallus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Gallus gallus | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | about 30% of the activity with Mg2+, at 5 mM and pH 7.4 | Gallus gallus | |
Mg2+ | best activation | Gallus gallus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
85000 | - |
x * 85000, SDS-PAGE | Gallus gallus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Gallus gallus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | bile canaliculi | Gallus gallus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1242 | - |
pH 7.4, 37°C | Gallus gallus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + H2O | - |
Gallus gallus | AMP + phosphate | - |
? | |
ATP + 2 H2O | - |
Gallus gallus | AMP + 2 phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 85000, SDS-PAGE | Gallus gallus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
substrate ADP | Gallus gallus |
8 | - |
substrate ATP | Gallus gallus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 6.5 | substrate ADP | Gallus gallus |
7.5 | 8.5 | substrate ATP | Gallus gallus |