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Literature summary for 3.6.1.5 extracted from
- Purification, characterization, cloning, and expression of the chicken liver ecto-ATP-diphosphohydrolase (2002), Eur. J. Biochem., 269, 2373-2382.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| NP-40 | 0.1%, about 3fold activation | Gallus gallus |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| azide | at 10 mM and pH 7.4, 21% inhibition of ATP hydrolysis, 85% inhibition of ADP hydrolysis | Gallus gallus | |
| diphosphate | at 10 mM and pH 7.4, 51% inhibition of ATP hydrolysis, 97% inhibition of ADP hydrolysis | Gallus gallus | |
| fluoride | at 10 mM and pH 7.4, 2% inhibition of ATP hydrolysis, 91% inhibition of ADP hydrolysis | Gallus gallus | |
| additional information | not inhibitory: Concanavalin A, suramin | Gallus gallus | |
| vanadate | at 10 mM and pH 7.4, 7% inhibition of ATP hydrolysis, 54% inhibition of ADP hydrolysis | Gallus gallus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.13 | - |
ATP | pH 6.4, 37°C | Gallus gallus | |
| 0.51 | - |
ATP | pH 7.4, 37°C | Gallus gallus | |
| 0.72 | - |
ADP | pH 6.4, 37°C | Gallus gallus | |
| 5.3 | - |
ADP | pH 7.4, 37°C | Gallus gallus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Gallus gallus | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | about 30% of the activity with Mg2+, at 5 mM and pH 7.4 | Gallus gallus | |
| Mg2+ | best activation | Gallus gallus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 85000 | - |
x * 85000, SDS-PAGE | Gallus gallus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gallus gallus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Gallus gallus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | bile canaliculi | Gallus gallus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1242 | - |
pH 7.4, 37°C | Gallus gallus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + H2O | - |
Gallus gallus | AMP + phosphate | - |
? | |
| ATP + 2 H2O | - |
Gallus gallus | AMP + 2 phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 85000, SDS-PAGE | Gallus gallus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
substrate ADP | Gallus gallus |
| 8 | - |
substrate ATP | Gallus gallus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 6.5 | substrate ADP | Gallus gallus |
| 7.5 | 8.5 | substrate ATP | Gallus gallus |
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