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Literature summary for 3.6.1.5 extracted from
- Purification and characterization of apyrase from the tick, Ornithodoros savignyi (1998), Comp. Biochem. Physiol. B, 120, 617-624.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| DTT | - |
Ornithodoros savignyi |  |
| EDTA | activity not restored with 5 mM Ca2+ or Mg2+ for periods up to 24h | Ornithodoros savignyi | |
| EGTA | - |
Ornithodoros savignyi | |
| HgCl | - |
Ornithodoros savignyi | |
| additional information | no inhibition by azide; no inhibition by orthovanadate; no inhibition by ouabain | Ornithodoros savignyi | |
| NaF | - |
Ornithodoros savignyi | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.76 | - |
ATP | - |
Ornithodoros savignyi | |
| 1.04 | - |
ADP | - |
Ornithodoros savignyi | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | only ADP hydrolysed in the presence of Ca2+ | Ornithodoros savignyi | |
| Mg2+ | - |
Ornithodoros savignyi | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 67000 | - |
SDS-PAGE | Ornithodoros savignyi |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + H2O | Ornithodoros savignyi | - |
AMP + phosphate | - |
? | |
| ATP + 2 H2O | Ornithodoros savignyi | - |
AMP + 2 phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ornithodoros savignyi | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Ornithodoros savignyi |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| salivary gland | - |
Ornithodoros savignyi | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 200 | - |
MgADP- | Ornithodoros savignyi |
| 280 | - |
CaADP- | Ornithodoros savignyi |
| 360 | - |
MgADP- | Ornithodoros savignyi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + H2O | - |
Ornithodoros savignyi | AMP + phosphate | - |
? | |
| ATP + 2 H2O | - |
Ornithodoros savignyi | AMP + 2 phosphate | - |
? | |
| additional information | neglible hydrolysis of AMP | Ornithodoros savignyi | ? | - |
? | |
| UDP + H2O | - |
Ornithodoros savignyi | UMP + phosphate | - |
? | |
| UTP + 2 H2O | - |
Ornithodoros savignyi | UMP + 2 phosphate | - |
? | |
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