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Literature summary for 3.6.1.5 extracted from

  • Cheeseman, M.T.
    Characterization of apyrase activity from the salivary glands of the cat flea Ctenocephalides felis (1998), Insect Biochem. Mol. Biol., 28, 1025-1030.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
Ctenocephalides felis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.066
-
ADP
-
Ctenocephalides felis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ Ca2+ preferred to Mg2+ Ctenocephalides felis
Mg2+
-
Ctenocephalides felis
Mn2+ lower activation than Ca2+ and Mg2+ Ctenocephalides felis
Zn2+ lower activation than Ca2+ ,Mg2+ and Mn2+ Ctenocephalides felis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
gel filtration Ctenocephalides felis
57000
-
gel filtration Ctenocephalides felis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ADP + H2O Ctenocephalides felis
-
AMP + phosphate
-
?
ATP + 2 H2O Ctenocephalides felis
-
AMP + 2 phosphate
-
?

Organism

Organism UniProt Comment Textmining
Ctenocephalides felis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partially Ctenocephalides felis

Source Tissue

Source Tissue Comment Organism Textmining
salivary gland
-
Ctenocephalides felis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + H2O
-
Ctenocephalides felis AMP + phosphate
-
?
ATP + 2 H2O
-
Ctenocephalides felis AMP + 2 phosphate
-
?
additional information neglible hydrolysis of AMP Ctenocephalides felis ?
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 9
-
Ctenocephalides felis