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Literature summary for 3.6.1.42 extracted from

  • Herrero, A.B.; Uccelletti, D.; Hirschberg, C.B.; Dominguez, A.; Abeijon, C.
    The Golgi GDPase of the fungal pathogen Candida albicans affects morphogenesis, glycosylation, and cell wall properties (2002), Eukaryot. Cell, 1, 420-431.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.1.42

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, functional complementation of a Saccharomyces GDPase null mutant Candida albicans

Protein Variants

Protein Variants Comment Organism
additional information disruption of gene gda1 leads to a mutant with 90% reduced GDP hydrolysis activity compared to the wild-type, the mutant also shows severely impaired O-mannosylation and reduced cell wall phosphate content and other phenotypes, but the formation of hyphae is uneffected, overview Candida albicans

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi apparatus
-
 Candida albicans 5794
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ stimulates Candida albicans
Mn2+ stimulates, preferred metal ion Candida albicans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GDP + H2O Candida albicans GDP hydrolysis occurs in the Golgi lumen to give GMP, which is required as antiporter for GDP-mannose transport into the Golgi lumen GMP + phosphate
-
 ?
additional information Candida albicans physiological function, role in O- and N-glycosylation, and cell and hyphae morphogenesis, affects the cell surface charge and phosphate content, enzyme is not required for the adhesion of Candida albicans to epithelial cells, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Candida albicans Q8TGH6 gene gda1
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Candida albicans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + H2O
-
 Candida albicans AMP + phosphate
-
 ?
GDP + H2O preferred substrate Candida albicans GMP + phosphate
-
 ?
GDP + H2O GDP hydrolysis occurs in the Golgi lumen to give GMP, which is required as antiporter for GDP-mannose transport into the Golgi lumen Candida albicans GMP + phosphate
-
 ?
additional information physiological function, role in O- and N-glycosylation, and cell and hyphae morphogenesis, affects the cell surface charge and phosphate content, enzyme is not required for the adhesion of Candida albicans to epithelial cells, overview Candida albicans ?
-
 ?
UDP + H2O
-
 Candida albicans UMP + phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer enzyme contains a unique sequence in the stem region predicted with high probability to adopt a coil-coil conformation that favors dimerization Candida albicans

Synonyms

Synonyms Comment Organism
Gdap1
-
 Candida albicans
GDPase
-
 Candida albicans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Candida albicans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Candida albicans