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Literature summary for 3.6.1.42 extracted from

  • Lopez-Avalos, M.D.; Uccelletti, D.; Abeijon, C.; Hirschberg, C.B.
    The UDPase activity of the Kluyveromyces lactis Golgi GDPase has a role in uridine nucleotide sugar transport into Golgi vesicles (2001), Glycobiology, 11, 413-422.
    View publication on PubMed
Search for more literature for 3.6.1.42

Cloned(Commentary)

Cloned (Comment) Organism
DNA sequence determination and analysis, enzyme can complement and restore activity in Saccharomyces cerevisiae deletion mutant gda1, complementation of N-glycosylation defects Kluyveromyces lactis

Protein Variants

Protein Variants Comment Organism
additional information enzyme null mutants show 95% reduced activity in vitro and inhibition of glycosyltransferases utilizing uridine and guanosine nucleotide sugars due to accumulation of nucleoside diphosphates, the transport of both GDP-mannose and UDP-GlcNAc into the Golgi lumen is reduced in the null mutants, complementation of the disruption mutant by recombinant expression of the wild-type enzyme or the Saccharomyces cerevisiae enzyme Kluyveromyces lactis

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi membrane with the catalytic domain being lumenal Kluyveromyces lactis 139
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ stimulates both GDPase and UDPase activity Kluyveromyces lactis
Mn2+ stimulates both GDPase and UDPase activity, stimulates UDPase activity by 5fold compared to stimulation with Ca2+ Kluyveromyces lactis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GDP + H2O Kluyveromyces lactis the enzyme's GDPase activity plays a role in guanosine nucleotide sugar transport into Golgi vesicles GMP + phosphate
-
 ?
additional information Kluyveromyces lactis physiological function, GMP and UMP are required as antiporters for guanosine and uridine nucleotide sugar transport into the Golgi lumen, involved in O-glycosylation ?
-
 ?
UDP + H2O Kluyveromyces lactis the enzyme's UDPase activity plays a role in uridine nucleotide sugar transport into Golgi vesicles UMP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Kluyveromyces lactis Q9HEM6 enzyme KlGdap1
-

Reaction

Reaction Comment Organism Reaction ID
GDP + H2O = GMP + phosphate also acts on UDP but not on other nucleoside diphosphates and triphosphates Kluyveromyces lactis
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 enzyme activity of wild-type and mutant enzyme in presence of Ca2+ or Mn2+ with substrates GDP or UDP Kluyveromyces lactis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP + H2O the enzyme's GDPase activity plays a role in guanosine nucleotide sugar transport into Golgi vesicles Kluyveromyces lactis GMP + phosphate
-
 ?
GDP + H2O preferred substrate in presence of Ca2+ or Mn2+ Kluyveromyces lactis GMP + phosphate
-
 ?
additional information physiological function, GMP and UMP are required as antiporters for guanosine and uridine nucleotide sugar transport into the Golgi lumen, involved in O-glycosylation Kluyveromyces lactis ?
-
 ?
additional information no activity with other nucleoside phosphates Kluyveromyces lactis ?
-
 ?
UDP + H2O
-
 Kluyveromyces lactis UMP + phosphate
-
 ?
UDP + H2O the enzyme's UDPase activity plays a role in uridine nucleotide sugar transport into Golgi vesicles Kluyveromyces lactis UMP + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
GDPase
-
 Kluyveromyces lactis
KlGdap1
-
 Kluyveromyces lactis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Kluyveromyces lactis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Kluyveromyces lactis