Cloned (Comment) | Organism |
---|---|
GDA1 gene | Saccharomyces cerevisiae |
overexpression of GDA1 gene | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi apparatus | distribution in multiple compartments | Saccharomyces cerevisiae | 5794 | - |
Golgi lumen | - |
Saccharomyces cerevisiae | 5796 | - |
Golgi vesicle | Golgi-like vesicles | Saccharomyces cerevisiae | - |
- |
membrane | type II membrane protein with a single hydrophobic stretch acting as an uncleaved signal sequence and anchor to the membrane preceded by a short hydrophilic, cytosolic tail | Saccharomyces cerevisiae | 16020 | - |
additional information | Golgi localization | Saccharomyces cerevisiae | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
deglycosylated GDPase, result of proteolytic cleavage of the 57000 Da enzyme during purification, SDS-PAGE | Saccharomyces cerevisiae |
56820 | - |
calculated from amino acid sequence | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP + H2O | Saccharomyces cerevisiae | GDPase plays a role in O- and N-mannosylation of proteins such as chitinase and carboxypeptidase Y and glycosylation of external invertase and of mannosylation of inositolphosphorylceramides | 5'-GMP + phosphate | - |
? | |
GDP + H2O | Saccharomyces cerevisiae | guanosine diphosphatase is required for protein and sphingolipid glycosylation in the Golgi lumen | 5'-GMP + phosphate | - |
? | |
additional information | Saccharomyces cerevisiae | guanosine diphosphatase is required for protein and sphingolipid glycosylation in the Golgi lumen | ? | - |
? | |
additional information | Saccharomyces cerevisiae | GDPase is required to complete O-glycosylation of proteins | ? | - |
? | |
additional information | Saccharomyces cerevisiae | GDPase not essential for cell viability and growth | ? | - |
? | |
additional information | Saccharomyces cerevisiae | GDPase plays a role in O- and N-mannosylation of proteins such as chitinase and carboxypeptidase Y and glycosylation of external invertase and of mannosylation of inositolphosphorylceramides and in synthesis of cell wall | ? | - |
? | |
additional information | Saccharomyces cerevisiae | GDPase plays an important role in mannosylation of proteins and lipids by regulating the amount of GDP-mannose available in Golgi lumen | ? | - |
? | |
additional information | Saccharomyces cerevisiae | GDPase has an important role in glycosylation reactions in Golgi lumen | ? | - |
? | |
additional information | Saccharomyces cerevisiae | role in mannosylation | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
wild-type and null mutant for guanosine diphosphatase | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | three potential N-glycosylation sites | Saccharomyces cerevisiae |
glycoprotein | high mannose glycoprotein | Saccharomyces cerevisiae |
proteolytic modification | probably proteolytic cleavage of the enzyme during purification | Saccharomyces cerevisiae |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP + H2O | highly specific for GDP | Saccharomyces cerevisiae | 5'-GMP + phosphate | - |
? | |
GDP + H2O | GDPase plays a role in O- and N-mannosylation of proteins such as chitinase and carboxypeptidase Y and glycosylation of external invertase and of mannosylation of inositolphosphorylceramides | Saccharomyces cerevisiae | 5'-GMP + phosphate | - |
? | |
GDP + H2O | guanosine diphosphatase is required for protein and sphingolipid glycosylation in the Golgi lumen | Saccharomyces cerevisiae | 5'-GMP + phosphate | - |
? | |
additional information | ADP: not a substrate | Saccharomyces cerevisiae | ? | - |
? | |
additional information | guanosine diphosphatase is required for protein and sphingolipid glycosylation in the Golgi lumen | Saccharomyces cerevisiae | ? | - |
? | |
additional information | GDPase is required to complete O-glycosylation of proteins | Saccharomyces cerevisiae | ? | - |
? | |
additional information | GDPase not essential for cell viability and growth | Saccharomyces cerevisiae | ? | - |
? | |
additional information | GDPase plays a role in O- and N-mannosylation of proteins such as chitinase and carboxypeptidase Y and glycosylation of external invertase and of mannosylation of inositolphosphorylceramides and in synthesis of cell wall | Saccharomyces cerevisiae | ? | - |
? | |
additional information | GDPase plays an important role in mannosylation of proteins and lipids by regulating the amount of GDP-mannose available in Golgi lumen | Saccharomyces cerevisiae | ? | - |
? | |
additional information | GDPase has an important role in glycosylation reactions in Golgi lumen | Saccharomyces cerevisiae | ? | - |
? | |
additional information | role in mannosylation | Saccharomyces cerevisiae | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Saccharomyces cerevisiae |