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Literature summary for 3.6.1.42 extracted from

  • Abeijon, C.; Yanagisawa, K.; Mandon, E.C.; Häusler, A.; Moremen, K.; Hirschberg, C.B.; Robbins, P.W.
    Guanosine diphosphatase is required for protein and sphingolipid glycosylation in the Golgi lumen of Saccharomyces cerevisiae (1993), J. Cell Biol., 122, 307-323.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
GDA1 gene Saccharomyces cerevisiae
overexpression of GDA1 gene Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi apparatus distribution in multiple compartments Saccharomyces cerevisiae 5794
-
Golgi lumen
-
Saccharomyces cerevisiae 5796
-
Golgi vesicle Golgi-like vesicles Saccharomyces cerevisiae
-
-
membrane type II membrane protein with a single hydrophobic stretch acting as an uncleaved signal sequence and anchor to the membrane preceded by a short hydrophilic, cytosolic tail Saccharomyces cerevisiae 16020
-
additional information Golgi localization Saccharomyces cerevisiae
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
deglycosylated GDPase, result of proteolytic cleavage of the 57000 Da enzyme during purification, SDS-PAGE Saccharomyces cerevisiae
56820
-
calculated from amino acid sequence Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GDP + H2O Saccharomyces cerevisiae GDPase plays a role in O- and N-mannosylation of proteins such as chitinase and carboxypeptidase Y and glycosylation of external invertase and of mannosylation of inositolphosphorylceramides 5'-GMP + phosphate
-
?
GDP + H2O Saccharomyces cerevisiae guanosine diphosphatase is required for protein and sphingolipid glycosylation in the Golgi lumen 5'-GMP + phosphate
-
?
additional information Saccharomyces cerevisiae guanosine diphosphatase is required for protein and sphingolipid glycosylation in the Golgi lumen ?
-
?
additional information Saccharomyces cerevisiae GDPase is required to complete O-glycosylation of proteins ?
-
?
additional information Saccharomyces cerevisiae GDPase not essential for cell viability and growth ?
-
?
additional information Saccharomyces cerevisiae GDPase plays a role in O- and N-mannosylation of proteins such as chitinase and carboxypeptidase Y and glycosylation of external invertase and of mannosylation of inositolphosphorylceramides and in synthesis of cell wall ?
-
?
additional information Saccharomyces cerevisiae GDPase plays an important role in mannosylation of proteins and lipids by regulating the amount of GDP-mannose available in Golgi lumen ?
-
?
additional information Saccharomyces cerevisiae GDPase has an important role in glycosylation reactions in Golgi lumen ?
-
?
additional information Saccharomyces cerevisiae role in mannosylation ?
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
wild-type and null mutant for guanosine diphosphatase
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein three potential N-glycosylation sites Saccharomyces cerevisiae
glycoprotein high mannose glycoprotein Saccharomyces cerevisiae
proteolytic modification probably proteolytic cleavage of the enzyme during purification Saccharomyces cerevisiae

Purification (Commentary)

Purification (Comment) Organism
-
Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP + H2O highly specific for GDP Saccharomyces cerevisiae 5'-GMP + phosphate
-
?
GDP + H2O GDPase plays a role in O- and N-mannosylation of proteins such as chitinase and carboxypeptidase Y and glycosylation of external invertase and of mannosylation of inositolphosphorylceramides Saccharomyces cerevisiae 5'-GMP + phosphate
-
?
GDP + H2O guanosine diphosphatase is required for protein and sphingolipid glycosylation in the Golgi lumen Saccharomyces cerevisiae 5'-GMP + phosphate
-
?
additional information ADP: not a substrate Saccharomyces cerevisiae ?
-
?
additional information guanosine diphosphatase is required for protein and sphingolipid glycosylation in the Golgi lumen Saccharomyces cerevisiae ?
-
?
additional information GDPase is required to complete O-glycosylation of proteins Saccharomyces cerevisiae ?
-
?
additional information GDPase not essential for cell viability and growth Saccharomyces cerevisiae ?
-
?
additional information GDPase plays a role in O- and N-mannosylation of proteins such as chitinase and carboxypeptidase Y and glycosylation of external invertase and of mannosylation of inositolphosphorylceramides and in synthesis of cell wall Saccharomyces cerevisiae ?
-
?
additional information GDPase plays an important role in mannosylation of proteins and lipids by regulating the amount of GDP-mannose available in Golgi lumen Saccharomyces cerevisiae ?
-
?
additional information GDPase has an important role in glycosylation reactions in Golgi lumen Saccharomyces cerevisiae ?
-
?
additional information role in mannosylation Saccharomyces cerevisiae ?
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
assay at Saccharomyces cerevisiae