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Literature summary for 3.6.1.41 extracted from
- Enzymatic characteristics of an ApaH-like phosphatase, PrpA, and a diadenosine tetraphosphate hydrolase, ApaH, from Myxococcus xanthus (2014), FEBS Lett., 588, 3395-3402.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 5 mM, 13.2% residual activity; 5 mM, 2.8% residual activity | Myxococcus xanthus |  |
| glycerol phosphate | 20 mM, 33.4% residual activity; 20 mM, 46.1% residual activity | Myxococcus xanthus | |
| iodoacetic acid | 10 mM, 58.8% residual activity | Myxococcus xanthus | |
| N-ethylmaleimide | 1 mM, 62.6% residual activity; 1 mM, 73.8% residual activity | Myxococcus xanthus | |
| sodium orthovanadate | 1 mM, 6.7% residual activity | Myxococcus xanthus | |
| trisodium phosphate | 20 mM, 24.8% residual activity; 20 mM, 6.2% residual activity | Myxococcus xanthus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.14 | - |
P1,P4-bis(adenosyl)tetraphosphate | pH 8.0, 50°C, presence of 1 mM Mn2+ | Myxococcus xanthus | |
| 0.19 | - |
P1,P5-bis(adenosyl)pentaphosphate | pH 8.0, 50°C, presence of 1 mM Mn2+ | Myxococcus xanthus | |
| 0.49 | - |
P1,P5-bis(adenosyl)pentaphosphate | pH 8.0, 50°C, presence of 1 mM Co2+ | Myxococcus xanthus | |
| 0.86 | - |
P1,P4-bis(adenosyl)tetraphosphate | pH 8.0, 50°C, presence of 1 mM Co2+ | Myxococcus xanthus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | about 50% of the activity with Mn2+. In the presence of Mn2+, PrpA hydrolyzes P1,P5-bis(adenosyl)tetraphosphate mainly into AMP and ATP, whereas in the presence of Co2+ PrpA hydrolyzes P1,P5-bis(adenosyl)tetraphosphate into two molecules of ADP, reaction of EC 3.6.1.17 | Myxococcus xanthus | |
| Co2+ | required for ApnA hydrolysis | Myxococcus xanthus | |
| Mn2+ | best activator. In the presence of Mn2+, PrpA hydrolyzes P1,P5-bis(adenosyl)tetraphosphate mainly into AMP and ATP, whereas in the presence of Co2+ PrpA hydrolyzes P1,P5-bis(adenosyl)tetraphosphate into two molecules of ADP, reaction of EC 3.6.1.17 | Myxococcus xanthus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Myxococcus xanthus | Q1CWE7 | - |
- |
| Myxococcus xanthus | Q1DC62 | - |
- |
| Myxococcus xanthus DK 1622 | Q1CWE7 | - |
- |
| Myxococcus xanthus DK 1622 | Q1DC62 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | enzyme exhibits high phosphatase activity towards 4-nitrophenyl phospahte, and hydrolase activity towards ApnA and ATP | Myxococcus xanthus | ? | - |
? | |
| additional information | PrpA exhibits a phosphatase activity towards p-nitrophenyl phosphate, tyrosine phosphopeptide and tyrosine-phosphorylated protein and a weak hydrolase activity towards ATP | Myxococcus xanthus | ? | - |
? | |
| additional information | enzyme exhibits high phosphatase activity towards 4-nitrophenyl phospahte, and hydrolase activity towards ApnA and ATP | Myxococcus xanthus DK 1622 | ? | - |
? | |
| additional information | PrpA exhibits a phosphatase activity towards p-nitrophenyl phosphate, tyrosine phosphopeptide and tyrosine-phosphorylated protein and a weak hydrolase activity towards ATP | Myxococcus xanthus DK 1622 | ? | - |
? | |
| P1,P4-bis(adenosyl)tetraphosphate + H2O | - |
Myxococcus xanthus | 2 ADP | - |
? | |
| P1,P4-bis(adenosyl)tetraphosphate + H2O | - |
Myxococcus xanthus | 2 ADP | in the presence of Mn2+, PrpA hydrolyzes Ap4A mainly into AMP and ATP, reaction of EC 3.6.1.17, whereas in the presence of Co2+, PrpA hydrolyzes Ap4A into two molecules of ADP | ? | |
| P1,P4-bis(adenosyl)tetraphosphate + H2O | - |
Myxococcus xanthus DK 1622 | 2 ADP | - |
? | |
| P1,P4-bis(adenosyl)tetraphosphate + H2O | - |
Myxococcus xanthus DK 1622 | 2 ADP | in the presence of Mn2+, PrpA hydrolyzes Ap4A mainly into AMP and ATP, reaction of EC 3.6.1.17, whereas in the presence of Co2+, PrpA hydrolyzes Ap4A into two molecules of ADP | ? | |
| P1,P5-bis(adenosyl)pentaphosphate + H2O | - |
Myxococcus xanthus | ADP + ATP | - |
? | |
| P1,P5-bis(adenosyl)pentaphosphate + H2O | - |
Myxococcus xanthus DK 1622 | ADP + ATP | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| APAH | - |
Myxococcus xanthus |
| MXAN_1509 | - |
Myxococcus xanthus |
| MXAN_7163 | - |
Myxococcus xanthus |
| PrpA | - |
Myxococcus xanthus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.035 | - |
P1,P5-bis(adenosyl)pentaphosphate | pH 8.0, 50°C, presence of 1 mM Mn2+ | Myxococcus xanthus | |
| 0.055 | - |
P1,P4-bis(adenosyl)tetraphosphate | pH 8.0, 50°C, presence of 1 mM Mn2+ | Myxococcus xanthus | |
| 7.12 | - |
P1,P4-bis(adenosyl)tetraphosphate | pH 8.0, 50°C, presence of 1 mM Co2+ | Myxococcus xanthus | |
| 24 | - |
P1,P5-bis(adenosyl)pentaphosphate | pH 8.0, 50°C, presence of 1 mM Co2+ | Myxococcus xanthus | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.18 | - |
P1,P5-bis(adenosyl)pentaphosphate | pH 8.0, 50°C, presence of 1 mM Mn2+ | Myxococcus xanthus | |
| 0.4 | - |
P1,P4-bis(adenosyl)tetraphosphate | pH 8.0, 50°C, presence of 1 mM Mn2+ | Myxococcus xanthus | |
| 8.28 | - |
P1,P4-bis(adenosyl)tetraphosphate | pH 8.0, 50°C, presence of 1 mM Co2+ | Myxococcus xanthus | |
| 48.57 | - |
P1,P5-bis(adenosyl)pentaphosphate | pH 8.0, 50°C, presence of 1 mM Co2+ | Myxococcus xanthus | |
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