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Literature summary for 3.6.1.40 extracted from

  • Keasling, J.D.; Bertsch, L.; Kornberg, A.
    Guanosine pentaphosphate phosphohydrolase of Escherichia coli is a long-chain exopolyphosphatase (1993), Proc. Natl. Acad. Sci. USA, 90, 7029-7033.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
KCl
-
Escherichia coli
NaCl
-
Escherichia coli
NaF
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0000005
-
Polyphosphate
-
Escherichia coli
0.13
-
guanosine 5'-triphosphate,3'-diphosphate
-
Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
SDS-PAGE Escherichia coli
100000
-
gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
guanosine 5'-triphosphate,3'-diphosphate + H2O Escherichia coli
-
guanosine 5'-diphosphate,3'-diphosphate + phosphate
-
?
guanosine 5'-triphosphate,3'-diphosphate + H2O Escherichia coli CA10
-
guanosine 5'-diphosphate,3'-diphosphate + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli CA10
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
22
-
NH4Cl precipitate Escherichia coli
7000
-
purified enzyme Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
guanosine 5'-triphosphate,3'-diphosphate + H2O
-
Escherichia coli guanosine 5'-diphosphate,3'-diphosphate + phosphate
-
?
guanosine 5'-triphosphate,3'-diphosphate + H2O
-
Escherichia coli CA10 guanosine 5'-diphosphate,3'-diphosphate + phosphate
-
?
additional information enzyme also liberates phosphate by processive hydrolysis of the phosphoanhydride bonds of polyphosphate chains (1000 residues) Escherichia coli ?
-
?
additional information enzyme also liberates phosphate by processive hydrolysis of the phosphoanhydride bonds of polyphosphate chains (1000 residues) Escherichia coli CA10 ?
-
?
polyphosphate + H2O
-
Escherichia coli ?
-
?
polyphosphate + H2O
-
Escherichia coli CA10 ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.023
-
guanosine 5'-triphosphate,3'-diphosphate
-
Escherichia coli
1.1
-
Polyphosphate
-
Escherichia coli