Protein Variants | Comment | Organism |
---|---|---|
H96G | site-directed mutagenesis in the fragile histidine triad, leads to reduced activity and reduced hydrolysis of reaction intermediates | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0029 | - |
P1-5'-O-adenosine-P3-5'-O-adenosine triphosphate | wild-type enzyme, pH 7.3 | Homo sapiens | |
0.0053 | - |
adenosine-5'-phospho-N-methylimidazolide | wild-type enzyme, pH 6.6 | Homo sapiens | |
0.0062 | - |
adenosine-5'-phosphoimidazolide | wild-type enzyme, pH 5.8 | Homo sapiens | |
0.026 | - |
adenosine-5'-phosphoimidazolide | mutant H96G, pH 5.8 | Homo sapiens | |
0.026 | - |
P1-5'-O-adenosine-P3-5'-O-adenosine triphosphate | mutant H96G, pH 8.9 | Homo sapiens | |
0.18 | - |
adenosine-5'-phospho-N-methylimidazolide | mutant H96G, pH 6.6 | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | dependent on, formation of an MgADP- intermediate during catalytic reaction | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
P1-5'-O-adenosine-P3-5'-O-adenosine triphosphate + H2O | Homo sapiens | - |
AMP + ADP | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
P1,P3-bis(5'-adenosyl) triphosphate + H2O = ADP + AMP | two-step reaction mechanism via a fragile histidine triad Fhit-AMP intermediate and release of MgADP-, kinetic of the reaction steps, overview | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenosine-5'-phospho-N-methylimidazolide + H2O | - |
Homo sapiens | ? | - |
? | |
adenosine-5'-phosphoimidazolide + H2O | - |
Homo sapiens | ? | - |
? | |
P1-5'-O-adenosine-P3-5'-O-adenosine triphosphate + H2O | - |
Homo sapiens | AMP + ADP | - |
r |
Synonyms | Comment | Organism |
---|---|---|
covalent adenylyl enzyme | - |
Homo sapiens |
Fhit | - |
Homo sapiens |
fragile histidine triad | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0014 | - |
P1-5'-O-adenosine-P3-5'-O-adenosine triphosphate | mutant H96G, pH 8.9 | Homo sapiens | |
0.62 | - |
adenosine-5'-phospho-N-methylimidazolide | mutant H96G, pH 6.6 | Homo sapiens | |
0.91 | - |
adenosine-5'-phosphoimidazolide | mutant H96G, pH 5.8 | Homo sapiens | |
3.5 | - |
P1-5'-O-adenosine-P3-5'-O-adenosine triphosphate | wild-type enzyme, pH 7.3 | Homo sapiens | |
4.2 | - |
adenosine-5'-phospho-N-methylimidazolide | wild-type enzyme, pH 6.6 | Homo sapiens | |
4.4 | - |
adenosine-5'-phosphoimidazolide | wild-type enzyme, pH 5.8 | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.8 | - |
optimal pH for hydrolysis of adenosine-5'-phosphoimidazolide | Homo sapiens |
6.6 | - |
optimal pH for hydrolysis of adenosine-5'-phospho-N-methylimidazolide | Homo sapiens |
7.3 | - |
optimal pH for hydrolysis of P1-5'-O-adenosine-P3-5'-O-adenosine triphosphate | Homo sapiens |