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Literature summary for 3.6.1.25 extracted from

  • Vogt, M.S.; Ngouoko Nguepbeu, R.R.; Mohr, M.K.F.; Albers, S.V.; Essen, L.O.; Banerjee, A.
    The archaeal triphosphate tunnel metalloenzyme SaTTM defines structural determinants for the diverse activities in the CYTH protein family (2021), J. Biol. Chem., 297, 100820.
    View publication on PubMed
Search for more literature for 3.6.1.25

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Sulfolobus acidocaldarius

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
 Sulfolobus acidocaldarius
diphosphate product inhibition Sulfolobus acidocaldarius
EDTA
-
 Sulfolobus acidocaldarius
additional information enzyme shows feedback inhibition due to tunnel closure in the product state Sulfolobus acidocaldarius

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.041
-
 Triphosphate pH 8.6, 75°C Sulfolobus acidocaldarius

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ preferred divalent cation Sulfolobus acidocaldarius
Mn2+ can partly substitue for Mg2+ Sulfolobus acidocaldarius
Ni2+ can partly substitue for Mg2+ Sulfolobus acidocaldarius
Zn2+ can partly substitue for Mg2+ Sulfolobus acidocaldarius

Organism

Organism UniProt Comment Textmining
Sulfolobus acidocaldarius Q4JAT2
-
-
Sulfolobus acidocaldarius DSM 639 Q4JAT2
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein, purified to homogeneity using an initial heat denaturation step followed by IMAC and subsequent size exclusion chromatography Sulfolobus acidocaldarius

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
 Sulfolobus acidocaldarius ADP + phosphate
-
 ?
ATP + H2O
-
 Sulfolobus acidocaldarius DSM 639 ADP + phosphate
-
 ?
additional information TTM dooes not produce any cyclic nucleotides. With substrate triphosphate, TTM is 19fold more reactive than with NTPs Sulfolobus acidocaldarius ?
-
-
additional information TTM dooes not produce any cyclic nucleotides. With substrate triphosphate, TTM is 19fold more reactive than with NTPs Sulfolobus acidocaldarius DSM 639 ?
-
-
tetraphosphate + 2 H2O
-
 Sulfolobus acidocaldarius diphosphate + 2 phosphate
-
 ?
tetraphosphate + 2 H2O
-
 Sulfolobus acidocaldarius DSM 639 diphosphate + 2 phosphate
-
 ?
triphosphate + H2O
-
 Sulfolobus acidocaldarius diphosphate + phosphate
-
 ?
triphosphate + H2O
-
 Sulfolobus acidocaldarius DSM 639 diphosphate + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
Saci_0718
-
 Sulfolobus acidocaldarius
TTM
-
 Sulfolobus acidocaldarius

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
-
 Sulfolobus acidocaldarius

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5934
-
 Triphosphate pH 8.6, 75°C Sulfolobus acidocaldarius

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.6
-
-
 Sulfolobus acidocaldarius

General Information

General Information Comment Organism
additional information enzyme shows a two-metal-ion reaction mechanism and feedback inhibition due to tunnel closure in the product state Sulfolobus acidocaldarius

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
145000
-
 Triphosphate pH 8.6, 75°C Sulfolobus acidocaldarius