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Literature summary for 3.6.1.25 extracted from

  • Swiezawska, B.; Duszyn, M.; Kwiatkowski, M.; Jaworski, K.; Pawelek, A.; Szmidt-Jaworska, A.
    Brachypodium distachyon triphosphate tunnel metalloenzyme 3 is both a triphosphatase and an adenylyl cyclase upregulated by mechanical wounding (2020), FEBS Lett., 594, 1101-1111.
    View publication on PubMed
Search for more literature for 3.6.1.25

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Brachypodium distachyon

Organism

Organism UniProt Comment Textmining
Brachypodium distachyon I1I2P2
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Brachypodium distachyon
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000006
-
 substrate ATP, formation of cAMP, presence of Mn2+, Mg2+, 30°C, pH 7.5 Brachypodium distachyon
0.007
-
 substrate ATP, release of ADP and phosphate, presence of Mn2+, Mg2+, 30°C, pH 7.5 Brachypodium distachyon
0.0115
-
 substrate triphosphate, presence of Mn2+, Mg2+, 30°C, pH 7.5 Brachypodium distachyon

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
 Brachypodium distachyon ADP + phosphate
-
 ?
additional information TTM3 is a protein with high triphosphatase activity (binding both tripolyphosphate and ATP) and low adenylyl cyclase activity Brachypodium distachyon ?
-
-
triphosphate + H2O
-
 Brachypodium distachyon diphosphate + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 23040, calculated from sequence, x * 55000, SDS-PAGE of recombinant GST-fusion protein Brachypodium distachyon

Synonyms

Synonyms Comment Organism
100822655
-
 Brachypodium distachyon
TTM3
-
 Brachypodium distachyon

pI Value

Organism Comment pI Value Maximum pI Value
Brachypodium distachyon calculated from sequence
-
 5.4

Expression

Organism Comment Expression
Brachypodium distachyon expression is induced by mechanical wounding up

General Information

General Information Comment Organism
additional information protein belongs to the CYTH-like phosphatase superfamily. The CYTH domain in BdTTM3 includes the characteristic EXEXK signature Brachypodium distachyon