Data extracted from this reference:
Activating Compound
Li2SO4
160% of standard activity at 0.75 M
Escherichia coli
NH4Cl
180% of standard activity at 1 M
Escherichia coli
General Stability
inactivation by repeated freezing and thawing
Escherichia coli
Inhibitors
alanyl adenylate
competitive inhibition of other substrates
Escherichia coli
aminoalkyl adenylate
strongest inhibitor, competitive inhibition of other substrates
Escherichia coli
AMP
competitive
Escherichia coli
KI
50% loss of activity at 1 M
Escherichia coli
lysyl adenylate
competitive inhibition of other substrates
Escherichia coli
Methionyl adenylate
competitive inhibition of other substrates
Escherichia coli
SDS
complete loss of activity at 6 mM
Escherichia coli
KM Value [mM]
0.056
adenosine 5'-p-nitrophenylphosphate
Escherichia coli
0.11
guanosine 5'-p-nitrophenylphosphate
Escherichia coli
0.65
uridine 5'-p-nitrophenylphosphate
Escherichia coli
4
cytidine 5'-p-nitrophenylphosphate
Escherichia coli
770
thymidine 5'-p-nitrophenylphosphate
Escherichia coli
Localization
additional information
not periplasm
Escherichia coli
Metals/Ions
additional information
no metal ions required
Escherichia coli
Molecular Weight [Da]
23000
sedimentation equilibrium method, gel filtration
Escherichia coli
Natural Substrates/ Products (Substrates)
p-nitrophenyl 5'-nucleotides + H2O
Escherichia coli
hydrolysis A,C,G,U,T nucleotides
p-nitrophenol + nucleoside phosphate
Escherichia coli
?
Organism
Oxidation Stability
stable towards reducing agents
Escherichia coli
Purification (Commentary)
Renatured (Commentary)
Specific Activity [micromol/min/mg]
0.0018
crude extract
Escherichia coli
6670
purified enzyme
Escherichia coli
Storage Stability
-20°C, can be stored for at least one year
Escherichia coli
Substrates and Products (Substrate)
acetyl adenylate + H2O
209999
Escherichia coli
?
?
adenosine 5'-p-nitrophenylphosphate + H2O
209999
Escherichia coli
p-nitrophenol + AMP
209999
Escherichia coli
?
cytidine 5'-p-nitrophenylphosphate + H2O
209999
Escherichia coli
p-nitrophenol + CMP
209999
Escherichia coli
?
guanosine 5'-p-nitrophenylphosphate + H2O
209999
Escherichia coli
p-nitrophenol + GMP
209999
Escherichia coli
?
methionyl adenylate + H2O
209999
Escherichia coli
?
?
p-nitrophenyl 5'-nucleotides + H2O
hydrolysis A,C,G,U,T nucleotides
209999
Escherichia coli
p-nitrophenol + nucleoside phosphate
209999
Escherichia coli
?
thymidine 5'-p-nitrophenylphosphate + H2O
209999
Escherichia coli
p-nitrophenol + TMP
209999
Escherichia coli
?
uridine 5'-p-nitrophenylphosphate + H2O
209999
Escherichia coli
p-nitrophenol + UMP
209999
Escherichia coli
?
Temperature Optimum [°C]
Temperature Range [°C]
10
65
rapid loss of activity above 50°C
Escherichia coli
Temperature Stability [°C]
no loss of activity within one day
Escherichia coli
65
loss of activity above 65°C, can be restored by cooling down to 0°C
Escherichia coli
pH Optimum
pH Range
5.5
10.5
very low activity at pH 5.5 and 10.5
Escherichia coli
pH Stability
7.5
irreversible inactivation below pH 7.5
Escherichia coli
Activating Compound (protein specific)
Li2SO4
160% of standard activity at 0.75 M
Escherichia coli
NH4Cl
180% of standard activity at 1 M
Escherichia coli
General Stability (protein specific)
inactivation by repeated freezing and thawing
Escherichia coli
Inhibitors (protein specific)
alanyl adenylate
competitive inhibition of other substrates
Escherichia coli
aminoalkyl adenylate
strongest inhibitor, competitive inhibition of other substrates
Escherichia coli
AMP
competitive
Escherichia coli
KI
50% loss of activity at 1 M
Escherichia coli
lysyl adenylate
competitive inhibition of other substrates
Escherichia coli
Methionyl adenylate
competitive inhibition of other substrates
Escherichia coli
SDS
complete loss of activity at 6 mM
Escherichia coli
KM Value [mM] (protein specific)
0.056
adenosine 5'-p-nitrophenylphosphate
Escherichia coli
0.11
guanosine 5'-p-nitrophenylphosphate
Escherichia coli
0.65
uridine 5'-p-nitrophenylphosphate
Escherichia coli
4
cytidine 5'-p-nitrophenylphosphate
Escherichia coli
770
thymidine 5'-p-nitrophenylphosphate
Escherichia coli
Localization (protein specific)
additional information
not periplasm
Escherichia coli
Metals/Ions (protein specific)
additional information
no metal ions required
Escherichia coli
Molecular Weight [Da] (protein specific)
23000
sedimentation equilibrium method, gel filtration
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
p-nitrophenyl 5'-nucleotides + H2O
Escherichia coli
hydrolysis A,C,G,U,T nucleotides
p-nitrophenol + nucleoside phosphate
Escherichia coli
?
Oxidation Stability (protein specific)
stable towards reducing agents
Escherichia coli
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
0.0018
crude extract
Escherichia coli
6670
purified enzyme
Escherichia coli
Storage Stability (protein specific)
-20°C, can be stored for at least one year
Escherichia coli
Substrates and Products (Substrate) (protein specific)
acetyl adenylate + H2O
209999
Escherichia coli
?
?
adenosine 5'-p-nitrophenylphosphate + H2O
209999
Escherichia coli
p-nitrophenol + AMP
209999
Escherichia coli
?
cytidine 5'-p-nitrophenylphosphate + H2O
209999
Escherichia coli
p-nitrophenol + CMP
209999
Escherichia coli
?
guanosine 5'-p-nitrophenylphosphate + H2O
209999
Escherichia coli
p-nitrophenol + GMP
209999
Escherichia coli
?
methionyl adenylate + H2O
209999
Escherichia coli
?
?
p-nitrophenyl 5'-nucleotides + H2O
hydrolysis A,C,G,U,T nucleotides
209999
Escherichia coli
p-nitrophenol + nucleoside phosphate
209999
Escherichia coli
?
thymidine 5'-p-nitrophenylphosphate + H2O
209999
Escherichia coli
p-nitrophenol + TMP
209999
Escherichia coli
?
uridine 5'-p-nitrophenylphosphate + H2O
209999
Escherichia coli
p-nitrophenol + UMP
209999
Escherichia coli
?
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
10
65
rapid loss of activity above 50°C
Escherichia coli
Temperature Stability [°C] (protein specific)
no loss of activity within one day
Escherichia coli
65
loss of activity above 65°C, can be restored by cooling down to 0°C
Escherichia coli
pH Optimum (protein specific)
pH Range (protein specific)
5.5
10.5
very low activity at pH 5.5 and 10.5
Escherichia coli
pH Stability (protein specific)
7.5
irreversible inactivation below pH 7.5
Escherichia coli
Other publictions for EC 3.6.1.24
209999
Spahr
Purification and properties of ...
Escherichia coli
Eur. J. Biochem.
12
270-284
1970
2
-
-
-
-
1
7
5
1
1
1
1
-
1
1
-
1
-
1
-
2
1
8
-
-
1
1
2
-
1
1
1
-
-
-
-
2
-
-
-
-
-
1
-
7
-
5
1
1
1
1
-
1
-
1
1
-
2
1
8
-
1
1
2
-
1
1
1
-
-
-
-
-
-
-