Purification and properties of a new enzyme from Escherichia coli: nucleoside phosphacyl hydrolase
Spahr, P.F.; Gesteland, R.F.; Eur. J. Biochem. 12, 270-284 (1970) 
Data extracted from this reference:
Activating Compound
Activating Compound
Commentary
Organism
Structure
Li2SO4
160% of standard activity at 0.75 M
Escherichia coli
NH4Cl
180% of standard activity at 1 M
Escherichia coli
General Stability
General Stability
Organism
inactivation by repeated freezing and thawing
Escherichia coli
Inhibitors
Inhibitors
Commentary
Organism
Structure
alanyl adenylate
competitive inhibition of other substrates
Escherichia coli
aminoalkyl adenylate
strongest inhibitor, competitive inhibition of other substrates
Escherichia coli
AMP
competitive
Escherichia coli
KI
50% loss of activity at 1 M
Escherichia coli
lysyl adenylate
competitive inhibition of other substrates
Escherichia coli
Methionyl adenylate
competitive inhibition of other substrates
Escherichia coli
SDS
complete loss of activity at 6 mM
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.056
-
adenosine 5'-p-nitrophenylphosphate
-
Escherichia coli
0.11
-
guanosine 5'-p-nitrophenylphosphate
-
Escherichia coli
0.65
-
uridine 5'-p-nitrophenylphosphate
-
Escherichia coli
4
-
cytidine 5'-p-nitrophenylphosphate
-
Escherichia coli
770
-
thymidine 5'-p-nitrophenylphosphate
-
Escherichia coli
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
additional information
not periplasm
Escherichia coli
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
no metal ions required
Escherichia coli
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
23000
-
sedimentation equilibrium method, gel filtration
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
p-nitrophenyl 5'-nucleotides + H2O
Escherichia coli
hydrolysis A,C,G,U,T nucleotides
p-nitrophenol + nucleoside phosphate
-
Escherichia coli
?
Organism
Organism
UniProt
Commentary
Textmining
Escherichia coli
-
-
-
Oxidation Stability
Oxidation Stability
Organism
stable towards reducing agents
Escherichia coli
Purification (Commentary)
Purification (Commentary)
Organism
-
Escherichia coli
Renatured (Commentary)
Renatured (Commentary)
Organism
-
Escherichia coli
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0018
-
crude extract
Escherichia coli
6670
-
purified enzyme
Escherichia coli
Storage Stability
Storage Stability
Organism
-20°C, can be stored for at least one year
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
acetyl adenylate + H2O
-
209999
Escherichia coli
?
-
-
-
?
adenosine 5'-p-nitrophenylphosphate + H2O
-
209999
Escherichia coli
p-nitrophenol + AMP
-
209999
Escherichia coli
?
cytidine 5'-p-nitrophenylphosphate + H2O
-
209999
Escherichia coli
p-nitrophenol + CMP
-
209999
Escherichia coli
?
guanosine 5'-p-nitrophenylphosphate + H2O
-
209999
Escherichia coli
p-nitrophenol + GMP
-
209999
Escherichia coli
?
methionyl adenylate + H2O
-
209999
Escherichia coli
?
-
-
-
?
p-nitrophenyl 5'-nucleotides + H2O
hydrolysis A,C,G,U,T nucleotides
209999
Escherichia coli
p-nitrophenol + nucleoside phosphate
-
209999
Escherichia coli
?
thymidine 5'-p-nitrophenylphosphate + H2O
-
209999
Escherichia coli
p-nitrophenol + TMP
-
209999
Escherichia coli
?
uridine 5'-p-nitrophenylphosphate + H2O
-
209999
Escherichia coli
p-nitrophenol + UMP
-
209999
Escherichia coli
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
-
Escherichia coli
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
10
65
rapid loss of activity above 50°C
Escherichia coli
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
-
-
no loss of activity within one day
Escherichia coli
65
-
loss of activity above 65°C, can be restored by cooling down to 0°C
Escherichia coli
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
8.5
-
Escherichia coli
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5.5
10.5
very low activity at pH 5.5 and 10.5
Escherichia coli
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
7.5
-
irreversible inactivation below pH 7.5
Escherichia coli
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
Li2SO4
160% of standard activity at 0.75 M
Escherichia coli
NH4Cl
180% of standard activity at 1 M
Escherichia coli
General Stability (protein specific)
General Stability
Organism
inactivation by repeated freezing and thawing
Escherichia coli
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
alanyl adenylate
competitive inhibition of other substrates
Escherichia coli
aminoalkyl adenylate
strongest inhibitor, competitive inhibition of other substrates
Escherichia coli
AMP
competitive
Escherichia coli
KI
50% loss of activity at 1 M
Escherichia coli
lysyl adenylate
competitive inhibition of other substrates
Escherichia coli
Methionyl adenylate
competitive inhibition of other substrates
Escherichia coli
SDS
complete loss of activity at 6 mM
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.056
-
adenosine 5'-p-nitrophenylphosphate
-
Escherichia coli
0.11
-
guanosine 5'-p-nitrophenylphosphate
-
Escherichia coli
0.65
-
uridine 5'-p-nitrophenylphosphate
-
Escherichia coli
4
-
cytidine 5'-p-nitrophenylphosphate
-
Escherichia coli
770
-
thymidine 5'-p-nitrophenylphosphate
-
Escherichia coli
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
additional information
not periplasm
Escherichia coli
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
no metal ions required
Escherichia coli
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
23000
-
sedimentation equilibrium method, gel filtration
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
p-nitrophenyl 5'-nucleotides + H2O
Escherichia coli
hydrolysis A,C,G,U,T nucleotides
p-nitrophenol + nucleoside phosphate
-
Escherichia coli
?
Oxidation Stability (protein specific)
Oxidation Stability
Organism
stable towards reducing agents
Escherichia coli
Purification (Commentary) (protein specific)
Commentary
Organism
-
Escherichia coli
Renatured (Commentary) (protein specific)
Commentary
Organism
-
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0018
-
crude extract
Escherichia coli
6670
-
purified enzyme
Escherichia coli
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, can be stored for at least one year
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
acetyl adenylate + H2O
-
209999
Escherichia coli
?
-
-
-
?
adenosine 5'-p-nitrophenylphosphate + H2O
-
209999
Escherichia coli
p-nitrophenol + AMP
-
209999
Escherichia coli
?
cytidine 5'-p-nitrophenylphosphate + H2O
-
209999
Escherichia coli
p-nitrophenol + CMP
-
209999
Escherichia coli
?
guanosine 5'-p-nitrophenylphosphate + H2O
-
209999
Escherichia coli
p-nitrophenol + GMP
-
209999
Escherichia coli
?
methionyl adenylate + H2O
-
209999
Escherichia coli
?
-
-
-
?
p-nitrophenyl 5'-nucleotides + H2O
hydrolysis A,C,G,U,T nucleotides
209999
Escherichia coli
p-nitrophenol + nucleoside phosphate
-
209999
Escherichia coli
?
thymidine 5'-p-nitrophenylphosphate + H2O
-
209999
Escherichia coli
p-nitrophenol + TMP
-
209999
Escherichia coli
?
uridine 5'-p-nitrophenylphosphate + H2O
-
209999
Escherichia coli
p-nitrophenol + UMP
-
209999
Escherichia coli
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
-
Escherichia coli
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
10
65
rapid loss of activity above 50°C
Escherichia coli
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
-
-
no loss of activity within one day
Escherichia coli
65
-
loss of activity above 65°C, can be restored by cooling down to 0°C
Escherichia coli
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
8.5
-
Escherichia coli
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5.5
10.5
very low activity at pH 5.5 and 10.5
Escherichia coli
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
7.5
-
irreversible inactivation below pH 7.5
Escherichia coli
Other publictions for EC 3.6.1.24
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
209999
Spahr
Purification and properties of ...
Escherichia coli
Eur. J. Biochem.
12
270-284
1970
2
-
-
-
-
1
7
5
1
1
1
1
-
1
1
-
1
-
1
-
2
1
8
-
-
1
1
2
-
1
1
1
-
-
-
-
2
-
-
-
-
-
1
-
7
-
5
1
1
1
1
-
1
-
1
1
-
2
1
8
-
1
1
2
-
1
1
1
-
-
-
-
-
-
-