Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 30% (w/v) PEG 1500 | Escherichia coli |
sitting drop vapor diffusion method, using 30% (w/v) PEG 1500 | white spot syndrome virus |
Protein Variants | Comment | Organism |
---|---|---|
D170G | the mutant shows 68% of wild type activity | white spot syndrome virus |
D170L | the mutant shows 1.7% of wild type activity | white spot syndrome virus |
D34N | the mutant shows 1.6% of wild type activity | white spot syndrome virus |
D88N | inactive | white spot syndrome virus |
DELTA134-153 | the mutant shows 0.73% of wild type activity | white spot syndrome virus |
DELTA161-170 | inactive | white spot syndrome virus |
DELTA170-171 | the mutant shows 14% of wild type activity | white spot syndrome virus |
F166Y | the mutant shows 5.5% of wild type activity | white spot syndrome virus |
K150A | the mutant shows 54% of wild type activity | white spot syndrome virus |
K150E | the mutant shows 49% of wild type activity | white spot syndrome virus |
R116S | the mutant shows 5.2% of wild type activity | Escherichia coli |
R158A | the mutant shows 28% of wild type activity | white spot syndrome virus |
R158E | the mutant shows 23% of wild type activity | white spot syndrome virus |
R158W | the mutant shows 22% of wild type activity | white spot syndrome virus |
R161K | the mutant shows 0.23% of wild type activity | white spot syndrome virus |
R24T | the mutant shows 3.6% of wild type activity | white spot syndrome virus |
R24T/S115R | the mutant shows 1.8% of wild type activity | white spot syndrome virus |
R24T/S115R/D170G | the mutant shows 17% of wild type activity | white spot syndrome virus |
R71E | inactive | white spot syndrome virus |
S115R | the mutant shows 13% of wild type activity | white spot syndrome virus |
T22R/R116S | the mutant shows 2.5% of wild type activity | Escherichia coli |
V160D | the mutant shows 85% of wild type activity | white spot syndrome virus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00093 | - |
dUTP | wild type enzyme, at pH 7.5 and 20°C | Escherichia coli | |
0.0017 | - |
dUTP | wild type enzyme, at pH 7.5 and 20°C | white spot syndrome virus | |
0.002 | - |
dUTP | mutant enzyme V160D, at pH 7.5 and 20°C | white spot syndrome virus | |
0.0021 | - |
dUTP | mutant enzyme D170G, at pH 7.5 and 20°C | white spot syndrome virus | |
0.0025 | - |
dUTP | mutant enzyme K150A, at pH 7.5 and 20°C | white spot syndrome virus | |
0.0026 | - |
dUTP | mutant enzyme K150E, at pH 7.5 and 20°C | white spot syndrome virus | |
0.0026 | - |
dUTP | mutant enzyme R158A, at pH 7.5 and 20°C | white spot syndrome virus | |
0.0026 | - |
dUTP | mutant enzyme R158E, at pH 7.5 and 20°C | white spot syndrome virus | |
0.0028 | - |
dUTP | mutant enzyme DELTA170-171, at pH 7.5 and 20°C | white spot syndrome virus | |
0.0029 | - |
dUTP | mutant enzyme R24T, at pH 7.5 and 20°C | white spot syndrome virus | |
0.0032 | - |
dUTP | mutant enzyme R158W, at pH 7.5 and 20°C | white spot syndrome virus | |
0.0042 | - |
dUTP | mutant enzyme R24T/S115R/D170G, at pH 7.5 and 20°C | white spot syndrome virus | |
0.0045 | - |
dUTP | mutant enzyme S115R, at pH 7.5 and 20°C | white spot syndrome virus | |
0.005 | - |
dUTP | mutant enzyme F166Y, at pH 7.5 and 20°C | white spot syndrome virus | |
0.0052 | - |
dUTP | mutant enzyme R166S, at pH 7.5 and 20°C | Escherichia coli | |
0.0054 | - |
dUTP | mutant enzyme R161K, at pH 7.5 and 20°C | white spot syndrome virus | |
0.0067 | - |
dUTP | mutant enzyme R24T/S115R, at pH 7.5 and 20°C | white spot syndrome virus | |
0.0071 | - |
dUTP | mutant enzyme D170L, at pH 7.5 and 20°C | white spot syndrome virus | |
0.013 | - |
dUTP | mutant enzyme D34N, at pH 7.5 and 20°C | white spot syndrome virus | |
0.013 | - |
dUTP | mutant enzyme T22R/R116S, at pH 7.5 and 20°C | Escherichia coli | |
0.021 | - |
dUTP | mutant enzyme DELTA134-153, at pH 7.5 and 20°C | white spot syndrome virus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Escherichia coli | |
Mg2+ | required | white spot syndrome virus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dUTP + H2O | Escherichia coli | - |
dUMP + diphosphate | - |
? | |
dUTP + H2O | white spot syndrome virus | - |
dUMP + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
white spot syndrome virus | Q77J78 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dUTP + H2O | - |
Escherichia coli | dUMP + diphosphate | - |
? | |
dUTP + H2O | - |
white spot syndrome virus | dUMP + diphosphate | - |
? | |
additional information | no activity with dUMP | Escherichia coli | ? | - |
- |
|
additional information | no activity with dUMP | white spot syndrome virus | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
homotrimer | - |
Escherichia coli |
homotrimer | - |
white spot syndrome virus |
Synonyms | Comment | Organism |
---|---|---|
DUT | - |
Escherichia coli |
DUT | - |
white spot syndrome virus |
dUTPase | - |
Escherichia coli |
dUTPase | - |
white spot syndrome virus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.053 | - |
dUTP | mutant enzyme R161K, at pH 7.5 and 20°C | white spot syndrome virus | |
0.45 | - |
dUTP | mutant enzyme R24T, at pH 7.5 and 20°C | white spot syndrome virus | |
0.51 | - |
dUTP | mutant enzyme R24T/S115R, at pH 7.5 and 20°C | white spot syndrome virus | |
0.53 | - |
dUTP | mutant enzyme D170L, at pH 7.5 and 20°C | white spot syndrome virus | |
0.67 | - |
dUTP | mutant enzyme DELTA134-153, at pH 7.5 and 20°C | white spot syndrome virus | |
0.92 | - |
dUTP | mutant enzyme D34N, at pH 7.5 and 20°C | white spot syndrome virus | |
1.2 | - |
dUTP | mutant enzyme F166Y, at pH 7.5 and 20°C | white spot syndrome virus | |
1.7 | - |
dUTP | mutant enzyme DELTA170-171, at pH 7.5 and 20°C | white spot syndrome virus | |
2.6 | - |
dUTP | mutant enzyme R158E, at pH 7.5 and 20°C | white spot syndrome virus | |
2.6 | - |
dUTP | mutant enzyme S115R, at pH 7.5 and 20°C | white spot syndrome virus | |
2.7 | - |
dUTP | mutant enzyme R166S, at pH 7.5 and 20°C | Escherichia coli | |
3.1 | - |
dUTP | mutant enzyme R158W, at pH 7.5 and 20°C | white spot syndrome virus | |
3.1 | - |
dUTP | mutant enzyme R24T/S115R/D170G, at pH 7.5 and 20°C | white spot syndrome virus | |
3.2 | - |
dUTP | mutant enzyme R158A, at pH 7.5 and 20°C | white spot syndrome virus | |
3.3 | - |
dUTP | mutant enzyme T22R/R116S, at pH 7.5 and 20°C | Escherichia coli | |
5.5 | - |
dUTP | mutant enzyme K150E, at pH 7.5 and 20°C | white spot syndrome virus | |
5.9 | - |
dUTP | mutant enzyme K150A, at pH 7.5 and 20°C | white spot syndrome virus | |
6.2 | - |
dUTP | mutant enzyme D170G, at pH 7.5 and 20°C | white spot syndrome virus | |
7.4 | - |
dUTP | wild type enzyme, at pH 7.5 and 20°C | white spot syndrome virus | |
7.4 | - |
dUTP | mutant enzyme V160D, at pH 7.5 and 20°C | white spot syndrome virus | |
9.3 | - |
dUTP | wild type enzyme, at pH 7.5 and 20°C | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.8 | - |
dUTP | mutant enzyme R161K, at pH 7.5 and 20°C | white spot syndrome virus | |
32 | - |
dUTP | mutant enzyme DELTA134-153, at pH 7.5 and 20°C | white spot syndrome virus | |
71 | - |
dUTP | mutant enzyme D34N, at pH 7.5 and 20°C | white spot syndrome virus | |
74 | - |
dUTP | mutant enzyme R24T/S115R/D170G, at pH 7.5 and 20°C | white spot syndrome virus | |
75 | - |
dUTP | mutant enzyme D170L, at pH 7.5 and 20°C | white spot syndrome virus | |
76 | - |
dUTP | mutant enzyme R24T/S115R, at pH 7.5 and 20°C | white spot syndrome virus | |
160 | - |
dUTP | mutant enzyme R24T, at pH 7.5 and 20°C | white spot syndrome virus | |
240 | - |
dUTP | mutant enzyme F166Y, at pH 7.5 and 20°C | white spot syndrome virus | |
250 | - |
dUTP | mutant enzyme T22R/R116S, at pH 7.5 and 20°C | Escherichia coli | |
520 | - |
dUTP | mutant enzyme R166S, at pH 7.5 and 20°C | Escherichia coli | |
580 | - |
dUTP | mutant enzyme S115R, at pH 7.5 and 20°C | white spot syndrome virus | |
610 | - |
dUTP | mutant enzyme DELTA170-171, at pH 7.5 and 20°C | white spot syndrome virus | |
970 | - |
dUTP | mutant enzyme R158W, at pH 7.5 and 20°C | white spot syndrome virus | |
1000 | - |
dUTP | mutant enzyme R158E, at pH 7.5 and 20°C | white spot syndrome virus | |
1200 | - |
dUTP | mutant enzyme R158A, at pH 7.5 and 20°C | white spot syndrome virus | |
2100 | - |
dUTP | mutant enzyme K150E, at pH 7.5 and 20°C | white spot syndrome virus | |
2400 | - |
dUTP | mutant enzyme K150A, at pH 7.5 and 20°C | white spot syndrome virus | |
3000 | - |
dUTP | mutant enzyme D170G, at pH 7.5 and 20°C | white spot syndrome virus | |
3700 | - |
dUTP | mutant enzyme V160D, at pH 7.5 and 20°C | white spot syndrome virus | |
4400 | - |
dUTP | wild type enzyme, at pH 7.5 and 20°C | white spot syndrome virus | |
10000 | - |
dUTP | wild type enzyme, at pH 7.5 and 20°C | Escherichia coli |