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Literature summary for 3.6.1.23 extracted from

  • Pecsi, I.; Hirmondo, R.; Brown, A.C.; Lopata, A.; Parish, T.; Vertessy, B.G.; Toth, J.
    The dUTPase enzyme is essential in Mycobacterium smegmatis (2012), PLoS ONE, 7, e37461.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.1.23

Application

Application Comment Organism
medicine dUTPase is a target for antitubercular drugs Mycobacterium tuberculosis
medicine dUTPase is a target for antitubercular drugs Mycolicibacterium smegmatis

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) (pLysS) cells Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
additional information the DELTA-loop deletion mutant is created by deletion of the five (Ala133-Ser137) loop-specific amino acids and shows reduced kcat and increased Km values for dUTP Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0009
-
 dUTP wild type enzyme, in 1 mM HEPES pH 7.5, 100 mM KCl, and 5 mM MgCl2, at 20°C Mycobacterium tuberculosis
0.0011
-
 dUTP DELTA-loop mutant enzyme, in 1 mM HEPES pH 7.5, 100 mM KCl, and 5 mM MgCl2, at 20°C Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dUTP + H2O Mycobacterium tuberculosis
-
 dUMP + diphosphate
-
 ?
dUTP + H2O Mycolicibacterium smegmatis
-
 dUMP + diphosphate
-
 ?
dUTP + H2O Mycolicibacterium smegmatis mc(2)155 / ATCC 700084
-
 dUMP + diphosphate
-
 ?
dUTP + H2O Mycobacterium tuberculosis H37Rv
-
 dUMP + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WNS5
-
-
Mycobacterium tuberculosis H37Rv P9WNS5
-
-
Mycolicibacterium smegmatis A0QW08
-
-
Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 A0QW08
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Mycobacterium tuberculosis
Ni-NTA column chromatography Mycolicibacterium smegmatis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dUTP + H2O
-
 Mycobacterium tuberculosis dUMP + diphosphate
-
 ?
dUTP + H2O
-
 Mycolicibacterium smegmatis dUMP + diphosphate
-
 ?
dUTP + H2O
-
 Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 dUMP + diphosphate
-
 ?
dUTP + H2O
-
 Mycobacterium tuberculosis H37Rv dUMP + diphosphate
-
 ?
additional information the enzyme cannot hydrolyze alpha-beta-imido-dUTP Mycobacterium tuberculosis ?
-
 ?
additional information the enzyme cannot hydrolyze alpha-beta-imido-dUTP Mycolicibacterium smegmatis ?
-
 ?
additional information the enzyme cannot hydrolyze alpha-beta-imido-dUTP Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 ?
-
 ?
additional information the enzyme cannot hydrolyze alpha-beta-imido-dUTP Mycobacterium tuberculosis H37Rv ?
-
 ?

Synonyms

Synonyms Comment Organism
deoxyuridine 5’-triphosphate nucleotidohydrolase
-
 Mycobacterium tuberculosis
deoxyuridine 5’-triphosphate nucleotidohydrolase
-
 Mycolicibacterium smegmatis
DUT
-
 Mycobacterium tuberculosis
DUT
-
 Mycolicibacterium smegmatis
dUTPase
-
 Mycobacterium tuberculosis
dUTPase
-
 Mycolicibacterium smegmatis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.88
-
 dUTP DELTA-loop mutant enzyme, in 1 mM HEPES pH 7.5, 100 mM KCl, and 5 mM MgCl2, at 20°C Mycobacterium tuberculosis
1.22
-
 dUTP wild type enzyme, in 1 mM HEPES pH 7.5, 100 mM KCl, and 5 mM MgCl2, at 20°C Mycobacterium tuberculosis

General Information

General Information Comment Organism
malfunction dUTPase knock-out results in lethality Mycolicibacterium smegmatis