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Literature summary for 3.6.1.23 extracted from

  • Palmen, L.G.; Kvassman, J.O.
    Differential inhibition of homotrimeric dUTPases by the 3-azido derivative of dideoxy-UTP (2010), J. Enzyme Inhib. Med. Chem., 25, 146-150.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLysS cells Escherichia coli
expressed in Escherichia coli BL21(DE3)pLysS cells Homo sapiens
expressed in Escherichia coli BL21(DE3)pLysS cells equine infectious anemia virus

Inhibitors

Inhibitors Comment Organism Structure
3'-azido-2',3'-dideoxy-UTP competitive inhibitor Escherichia coli
additional information the enzyme is not inhibited by 3'-azido-2',3'-dideoxy-UTP equine infectious anemia virus
additional information the enzyme is not inhibited by 3'-azido-2',3'-dideoxy-UTP Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00017
-
dUTP pH and temperature not specified in the publication Homo sapiens
0.00022
-
dUTP pH and temperature not specified in the publication Escherichia coli
0.00096
-
dUTP pH and temperature not specified in the publication equine infectious anemia virus

Organism

Organism UniProt Comment Textmining
equine infectious anemia virus
-
-
-
Escherichia coli
-
-
-
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
phosphocellulose column chromatography Escherichia coli
phosphocellulose column chromatography Homo sapiens
phosphocellulose column chromatography equine infectious anemia virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dUTP + H2O
-
Escherichia coli dUMP + diphosphate
-
?
dUTP + H2O
-
Homo sapiens dUMP + diphosphate
-
?
dUTP + H2O
-
equine infectious anemia virus dUMP + diphosphate
-
?

Subunits

Subunits Comment Organism
homotrimer
-
Escherichia coli
homotrimer
-
Homo sapiens
homotrimer
-
equine infectious anemia virus

Synonyms

Synonyms Comment Organism
dUTPase
-
Escherichia coli
dUTPase
-
Homo sapiens
dUTPase
-
equine infectious anemia virus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0093
-
3'-azido-2',3'-dideoxy-UTP 0.02 mM 3'-azido-2',3'-dideoxy-UTP in 0.05 mM bicine, with 5 mM MgCl2, pH and temperature not specified in the publication Escherichia coli