Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)pLysS cells | Escherichia coli |
expressed in Escherichia coli BL21(DE3)pLysS cells | Homo sapiens |
expressed in Escherichia coli BL21(DE3)pLysS cells | equine infectious anemia virus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3'-azido-2',3'-dideoxy-UTP | competitive inhibitor | Escherichia coli | |
additional information | the enzyme is not inhibited by 3'-azido-2',3'-dideoxy-UTP | equine infectious anemia virus | |
additional information | the enzyme is not inhibited by 3'-azido-2',3'-dideoxy-UTP | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00017 | - |
dUTP | pH and temperature not specified in the publication | Homo sapiens | |
0.00022 | - |
dUTP | pH and temperature not specified in the publication | Escherichia coli | |
0.00096 | - |
dUTP | pH and temperature not specified in the publication | equine infectious anemia virus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
equine infectious anemia virus | - |
- |
- |
Escherichia coli | - |
- |
- |
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
phosphocellulose column chromatography | Escherichia coli |
phosphocellulose column chromatography | Homo sapiens |
phosphocellulose column chromatography | equine infectious anemia virus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dUTP + H2O | - |
Escherichia coli | dUMP + diphosphate | - |
? | |
dUTP + H2O | - |
Homo sapiens | dUMP + diphosphate | - |
? | |
dUTP + H2O | - |
equine infectious anemia virus | dUMP + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotrimer | - |
Escherichia coli |
homotrimer | - |
Homo sapiens |
homotrimer | - |
equine infectious anemia virus |
Synonyms | Comment | Organism |
---|---|---|
dUTPase | - |
Escherichia coli |
dUTPase | - |
Homo sapiens |
dUTPase | - |
equine infectious anemia virus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0093 | - |
3'-azido-2',3'-dideoxy-UTP | 0.02 mM 3'-azido-2',3'-dideoxy-UTP in 0.05 mM bicine, with 5 mM MgCl2, pH and temperature not specified in the publication | Escherichia coli |