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Literature summary for 3.6.1.23 extracted from

  • Toth, J.; Varga, B.; Kovacs, M.; Malnasi-Csizmadia, A.; Vertessy, B.G.
    Kinetic mechanism of human dUTPase, an essential nucleotide pyrophosphatase enzyme (2007), J. Biol. Chem., 282, 33572-33582.
    View publication on PubMed
Search for more literature for 3.6.1.23

Application

Application Comment Organism
drug development the nuclear isoform of the enzyme is a target for anticancer chemotherapeutic strategies Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression of the His-tagged nuclear isoform and its F158W mutant Homo sapiens

Protein Variants

Protein Variants Comment Organism
F158W site-directed mutagenesis, inactive mutant Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
alpha,beta-imido-dUTP
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information detailed pre-steady-state and steady-state kinetic analysis, quantitative kinetic model of the human dUTPase catalytic cycle, overview Homo sapiens
0.0036
-
 dUTP pH 7.4, 20°C, recombinant wild-type enzyme Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus nuclear isozyme Homo sapiens 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dUTP + H2O Homo sapiens dUTPase is essential in controlling relative cellular levels of dTTP/dUTP, both of which can be incorporated into DNA dUMP + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P33316
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dUTP + H2O dUTPase is essential in controlling relative cellular levels of dTTP/dUTP, both of which can be incorporated into DNA Homo sapiens dUMP + diphosphate
-
 ?
dUTP + H2O substrate synthesis, overview, the enzymatic cycle is limited by a single rate-limiting step that occurs before or is concomitant with proton release Homo sapiens dUMP + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
dUTPase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
 assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.58
-
 dUTP pH 7.4, 20°C, recombinant wild-type enzyme Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens