Application | Comment | Organism |
---|---|---|
drug development | dUTPase is a promising antituberculotic drug target | Mycobacterium tuberculosis |
Cloned (Comment) | Organism |
---|---|
expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
dUTPase in complex with the isosteric substrate analogue, alpha,beta-imido-dUTP, and Mg2+, about 0.223 mM dUTPase, 1.25 mM alpha,beta-imido-dUTP and 10 mM MgCl2 in 10 mM Tris-HCl, pH7.0, 50 mM NaCl, and 0.1 mM TCEP buffer is mixed with different reservoir solutions, X-ray diffraction structure determination and analysis at 1.5 A resolution, molecular replacement | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
H145W | site-directed mutagenesis, the replacement introduces a 244 sensitive fluorescent label into the binding site. The steady-state kinetics show no difference in the case of the mutant and wild-type enzyme | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | fast screening for binding of potential inhibitors to the active site | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, binding structure, overview | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dUTP + H2O | Mycobacterium tuberculosis | dUTPase is essential to eliminate dUTP for DNA integrity and provide dUMP for thymidylate biosynthesis | dUMP + diphosphate | - |
? | |
dUTP + H2O | Mycobacterium tuberculosis H37Rv | dUTPase is essential to eliminate dUTP for DNA integrity and provide dUMP for thymidylate biosynthesis | dUMP + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WNS5 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WNS5 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dUTP + H2O | dUTPase is essential to eliminate dUTP for DNA integrity and provide dUMP for thymidylate biosynthesis | Mycobacterium tuberculosis | dUMP + diphosphate | - |
? | |
dUTP + H2O | development of a robust and continuous dUTPase enzyme activity assay method using phenolred indicator, overview | Mycobacterium tuberculosis | dUMP + diphosphate | - |
? | |
dUTP + H2O | dUTPase is essential to eliminate dUTP for DNA integrity and provide dUMP for thymidylate biosynthesis | Mycobacterium tuberculosis H37Rv | dUMP + diphosphate | - |
? | |
dUTP + H2O | development of a robust and continuous dUTPase enzyme activity assay method using phenolred indicator, overview | Mycobacterium tuberculosis H37Rv | dUMP + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
dUTPase | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.7 | 5.8 | dUTP | pH 7.5, 25°C, recombinant wild-type and mutant enzymes | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Mycobacterium tuberculosis |