Cloned (Comment) | Organism |
---|---|
enzyme expression | Arabidopsis thaliana |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme, hanging drop vapour diffusion method, 25°C, 0.001 ml of protein solution containing 10 mg/ml protein in 50 mM Tris-HCl, pH 7.4, is mixed with 0.001 ml of reservoir solution containing 2 M ammonium sulfate and 0.1 M Tris-HCl, pH 8.5, X-ray diffraction structure determination and analysis at 2.2-2.3 A resolution, molecular replacement | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dUTP + H2O | Arabidopsis thaliana | the enzyme is responsible for maintaining a low dUTP level in order to avoid the incorporation of uracil into DNA, and for providing dUMP as a substrate for deoxythymidine triphosphate biosynthesis | dUMP + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9STG6 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from to homogeneity | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dUTP + H2O | - |
Arabidopsis thaliana | dUMP + diphosphate | - |
? | |
dUTP + H2O | the enzyme is responsible for maintaining a low dUTP level in order to avoid the incorporation of uracil into DNA, and for providing dUMP as a substrate for deoxythymidine triphosphate biosynthesis | Arabidopsis thaliana | dUMP + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
deoxyuridine triphosphate nucleotidohydrolase | - |
Arabidopsis thaliana |
dUTPase | - |
Arabidopsis thaliana |