Cloned (Comment) | Organism |
---|---|
gene yosS encoded by the Bacillus subtilis temperate bacteriophage SPbeta, DNA and amino acid sequence determination and analysis, functional overexpression in Escherichia coli strain BL21(DE3) | Spbetavirus SPbeta |
Crystallization (Comment) | Organism |
---|---|
purified recombinant free enzyme or recombinant enzyme complexed with substrate dUTP, hanging drop vapour diffusion method, protein and reservoir solution in a 1:1 ratio, 17°C, the reservoir solution contains 0.1 M HEPES, pH 7.5, 10% w/v PEG 8000, and 8% v/v ethylene glycol, 2 days, X-ray diffraction structure determination and analysis at 2.0 A resolution | Spbetavirus SPbeta |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | - |
Spbetavirus SPbeta | |
Ca2+ | - |
Spbetavirus SPbeta | |
Cd2+ | - |
Spbetavirus SPbeta |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0006 | - |
dUTP | pH 7.5, 25°C, recombinant enzyme | Spbetavirus SPbeta |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activates to 72% of the activity with Mg2+ | Spbetavirus SPbeta | |
Mg2+ | required, best divalent cation | Spbetavirus SPbeta | |
Mn2+ | activates to 68% of the activity with Mg2+ | Spbetavirus SPbeta | |
additional information | no effect by Zn2+ | Spbetavirus SPbeta | |
Ni2+ | activates to 49% of the activity with Mg2+ | Spbetavirus SPbeta |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
16000 | - |
3 * 16000, recombinant enzyme, SDS-PAGE | Spbetavirus SPbeta |
48000 | - |
recombinant enzyme, gel filtration | Spbetavirus SPbeta |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dUTP + H2O | Spbetavirus SPbeta | - |
dUMP + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spbetavirus SPbeta | - |
gene yosS | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme of Bacillus subtilis temperate bacteriophage SPbeta from Escherichia coli strain BL21(DE3) by phosphocellulose chromatography to homogeneity | Spbetavirus SPbeta |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Spbetavirus SPbeta |
69 | - |
purified enzyme | Spbetavirus SPbeta |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dUTP + H2O | - |
Spbetavirus SPbeta | dUMP + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 16000, recombinant enzyme, SDS-PAGE | Spbetavirus SPbeta |
Synonyms | Comment | Organism |
---|---|---|
dUTPase | - |
Spbetavirus SPbeta |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Spbetavirus SPbeta |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
18 | - |
dUTP | pH 7.5, 25°C, recombinant enzyme | Spbetavirus SPbeta |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Spbetavirus SPbeta |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Spbetavirus SPbeta | isoelectric focusing | - |
6.7 |