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Literature summary for 3.6.1.23 extracted from
- Chlorella virus-encoded deoxyuridine triphosphatases exhibit different temperature optima (2005), J. Virol., 79, 9945-9953.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Paramecium bursaria Chlorella virus 1 |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| dUTP | substrate inhibition at higher concentrations | Paramecium bursaria Chlorella virus 1 |  |
| additional information | no inhibition by dTTp, dGTP, dATP, and dCTP | Paramecium bursaria Chlorella virus 1 |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0117 | - |
dUTP | pH 8.5, 50°C, recombinant enzyme | Paramecium bursaria Chlorella virus 1 | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | best divalent cation, bound at the center of the trimeric enzyme structure | Paramecium bursaria Chlorella virus 1 | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54000 | - |
recombinant His-tagged enzyme, native PAGE | Paramecium bursaria Chlorella virus 1 |
| 176000 | - |
3 * 176000, recombinant His-tagged enzyme, SDS-PAGE | Paramecium bursaria Chlorella virus 1 |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dUTP + H2O | Paramecium bursaria Chlorella virus 1 | dUMP is required for biosynthesis of dTTP, overview | dUMP + diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paramecium bursaria Chlorella virus 1 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Paramecium bursaria Chlorella virus 1 |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| dUTP + H2O = dUMP + diphosphate | active site structure, R84 and S81 are involved in substrate binding, molecular modeling | Paramecium bursaria Chlorella virus 1 |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Paramecium bursaria Chlorella virus 1 |
| 23.9 | - |
purified recombinant enzyme | Paramecium bursaria Chlorella virus 1 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dUTP + H2O | dUMP is required for biosynthesis of dTTP, overview | Paramecium bursaria Chlorella virus 1 | dUMP + diphosphate | - |
? | |
| dUTP + H2O | the enzyme is highly specific for dUTP | Paramecium bursaria Chlorella virus 1 | dUMP + diphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | molecular structure modeling | Paramecium bursaria Chlorella virus 1 |
| trimer | 3 * 176000, recombinant His-tagged enzyme, SDS-PAGE | Paramecium bursaria Chlorella virus 1 |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| deoxyuridine triphosphatase | - |
Paramecium bursaria Chlorella virus 1 |
| dUTPase | - |
Paramecium bursaria Chlorella virus 1 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Paramecium bursaria Chlorella virus 1 |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 65 | active range | Paramecium bursaria Chlorella virus 1 |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 6.8 | - |
dUTP | pH 8.5, 50°C, recombinant enzyme | Paramecium bursaria Chlorella virus 1 | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Paramecium bursaria Chlorella virus 1 |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2 | 11 | active range | Paramecium bursaria Chlorella virus 1 |
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