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Literature summary for 3.6.1.23 extracted from

  • Roseman, N.A.; Evans, R.K.; Mayer, E.L.; Rossi, M.A.; Slabaugh, M.B.
    Purification and characterization of the vaccinia virus deoxyuridine triphosphatase expressed in Escherichia coli (1996), J. Biol. Chem., 271, 23506-23511.
    View publication on PubMed
Search for more literature for 3.6.1.23

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli under the control of a bacteriophage T7 promoter Vaccinia virus

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
 Vaccinia virus
8-azido-ATP competitive Vaccinia virus
EDTA reversed by Mg2+, Mn2+ or Zn2+ Vaccinia virus
EGTA
-
 Vaccinia virus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00094
-
 dUTP
-
 Vaccinia virus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
16400
-
 3 * 16400, calculation from amino acid sequence Vaccinia virus
45000
-
 gel filtration Vaccinia virus

Organism

Organism UniProt Comment Textmining
Vaccinia virus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Vaccinia virus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Vaccinia virus

Storage Stability

Storage Stability Organism
-80°C, stable for 1 year Vaccinia virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dUTP + H2O
-
 Vaccinia virus dUMP + diphosphate
-
 ?

Subunits

Subunits Comment Organism
trimer 3 * 16400, calculation from amino acid sequence Vaccinia virus