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Literature summary for 3.6.1.23 extracted from

  • Price, A.R.; Frato, J.
    Bacillus subtilis deoxyuridinetriphosphatase and its bacteriophage PBS2-induced inhibitor (1975), J. Biol. Chem., 250, 8804-8811.
    View publication on PubMed
Search for more literature for 3.6.1.23

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 50-500 mM, 25% stimulation Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
bacteriophage PBS2-induced inhibitor seems to form a high MW complex with the Bacillus subtilis dUTPase, the lower MW enzyme form of dUTPase is not inactivated by the protease inhibitor Bacillus subtilis
EDTA
-
 Bacillus subtilis
Hg2+ 10 mM HgCl2, 50% inhibition in absence of mercaptoethanol Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
 dUTP
-
 Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ MgCl2 stimulates Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
 low molecular weight enzyme form, gel filtration Bacillus subtilis
48000
-
 high molecular weight enzyme form, gel filtration Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dUTP + H2O
-
 Bacillus subtilis dUMP + diphosphate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
8 8.5
-
 Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5
-
 Bacillus subtilis

pH Range

pH Minimum pH Maximum Comment Organism
6 10 50% of maximal activity at pH 6 and pH 10 Bacillus subtilis