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Literature summary for 3.6.1.22 extracted from
- Cloning and characterization of the NADH pyrophospatases from Caenorhabditis elegans and Saccharomyces cerevisiae, members of a nudix hydrolase subfamily (2000), Biochem. Biophys. Res. Commun., 273, 753-758.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Saccharomyces cerevisiae |
| expressed in Escherichia coli | Caenorhabditis elegans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | in presence of Mg2+ complete inhibition | Caenorhabditis elegans |  |
| Ca2+ | in presence of Mg2+ complete inhibition | Saccharomyces cerevisiae | |
| Zn2+ | in presence of Mg2+ complete inhibition | Caenorhabditis elegans | |
| Zn2+ | in presence of Mg2+ complete inhibition | Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.4 | - |
NADH | 50 mM Tris-HCl, pH 8.5, 5 mM MgCl2 | Caenorhabditis elegans | |
| 1.6 | - |
NADH | 50 mM Tris-HCl, pH 7.5, 5 mM MgCl2 | Saccharomyces cerevisiae | |
| 6.6 | - |
NAD+ | 50 mM Tris-HCl, pH 8.5, 5 mM MgCl2 | Caenorhabditis elegans | |
| 6.6 | - |
NAD+ | 50 mM Tris-HCl, pH 7.5, 5 mM MgCl2 | Saccharomyces cerevisiae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | strong activation | Escherichia coli | |
| Mg2+ | strong activation | Saccharomyces cerevisiae | |
| Mg2+ | strong activation | Caenorhabditis elegans | |
| Mn2+ | strong activation | Escherichia coli | |
| Mn2+ | strong activation | Saccharomyces cerevisiae | |
| Mn2+ | strong activation | Caenorhabditis elegans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39100 | - |
homodimer, 2 * 39100 | Caenorhabditis elegans |
| 43500 | - |
homodimer, 2 * 43500 | Saccharomyces cerevisiae |
| 78200 | - |
two subunits of 39100 Da found | Caenorhabditis elegans |
| 87000 | - |
two subunits of 43500 Da found | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NADH + H2O | Escherichia coli | possible function in regulation of NAD+/NADH-ratio | NMNH + AMP | - |
ir | |
| NADH + H2O | Saccharomyces cerevisiae | possible function in regulation of NAD+/NADH-ratio | NMNH + AMP | - |
ir | |
| NADH + H2O | Caenorhabditis elegans | possible function in regulation of NAD+/NADH-ratio | NMNH + AMP | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Caenorhabditis elegans | - |
- |
- |
| Escherichia coli | - |
- |
- |
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purification of gene product from Escherichia coli | Saccharomyces cerevisiae |
| purification of gene product from Escherichia coli | Caenorhabditis elegans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADPribose + H2O | - |
Escherichia coli | ? | - |
? | |
| ADPribose + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
| ADPribose + H2O | - |
Caenorhabditis elegans | ? | - |
? | |
| AppA + H2O | - |
Escherichia coli | ? | - |
? | |
| AppA + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
| AppA + H2O | - |
Caenorhabditis elegans | ? | - |
? | |
| dATP + H2O | - |
Caenorhabditis elegans | ? | - |
? | |
| NAD+ + H2O | - |
Escherichia coli | NMN + AMP | - |
ir | |
| NAD+ + H2O | - |
Saccharomyces cerevisiae | NMN + AMP | - |
ir | |
| NAD+ + H2O | - |
Caenorhabditis elegans | NMN + AMP | - |
ir | |
| NADH + H2O | possible function in regulation of NAD+/NADH-ratio | Escherichia coli | NMNH + AMP | - |
ir | |
| NADH + H2O | possible function in regulation of NAD+/NADH-ratio | Saccharomyces cerevisiae | NMNH + AMP | - |
ir | |
| NADH + H2O | possible function in regulation of NAD+/NADH-ratio | Caenorhabditis elegans | NMNH + AMP | - |
ir | |
| NADH + H2O | - |
Escherichia coli | AMP + NMNH | - |
? | |
| NADH + H2O | - |
Saccharomyces cerevisiae | AMP + NMNH | - |
? | |
| NADH + H2O | - |
Caenorhabditis elegans | AMP + NMNH | - |
? | |
| P1,P4-bis(5'-adenosyl) tetraphosphate + H2O | - |
Escherichia coli | ? | - |
? | |
| P1,P4-bis(5'-adenosyl) tetraphosphate + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
| P1,P4-bis(5'-adenosyl) tetraphosphate + H2O | - |
Caenorhabditis elegans | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Escherichia coli |
| dimer | homodimer, 2 * 39100 | Caenorhabditis elegans |
| dimer | homodimer, 2 * 43500 | Saccharomyces cerevisiae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000167 | - |
NAD+ | - |
Saccharomyces cerevisiae | |
| 0.000167 | - |
NAD+ | - |
Caenorhabditis elegans | |
| 0.00117 | - |
NADH | - |
Caenorhabditis elegans | |
| 0.0025 | - |
NADH | - |
Saccharomyces cerevisiae | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Saccharomyces cerevisiae |
| 8.5 | - |
- |
Escherichia coli |
| 8.5 | - |
- |
Caenorhabditis elegans |
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