BRENDA - Enzyme Database
show all sequences of 3.6.1.21

Uridine diphosphate sugar hydrolase. Purification of enzyme and protein inhibitor

Glaser, L.; Melo, A.; Paul, R.; J. Biol. Chem. 242, 1944-1954 (1967)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.9
-
ADP-Glc
-
Salmonella enterica subsp. enterica serovar Typhimurium
40
-
GDP-Glc
same Km for GDP-Glc and GDP-Man
Salmonella enterica subsp. enterica serovar Typhimurium
40
-
GDP-Man
same Km for GDP-Glc and GDP-Man
Salmonella enterica subsp. enterica serovar Typhimurium
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Escherichia coli
-
-
soluble
-
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Salmonella enterica subsp. enterica serovar Typhimurium
Mg2+
-
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ADP-sugar + H2O
Salmonella enterica subsp. enterica serovar Typhimurium
-
AMP + sugar phosphate
-
Salmonella enterica subsp. enterica serovar Typhimurium
?
ADP-sugar + H2O
Escherichia coli
-
AMP + sugar phosphate
-
Escherichia coli
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
strain ATCC 12793
-
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-
Purification (Commentary)
Commentary
Organism
partial purification together with UDP-sugar pyrophosphatase
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ADP-sugar + H2O
-
209944
Salmonella enterica subsp. enterica serovar Typhimurium
AMP + sugar phosphate
-
209944
Salmonella enterica subsp. enterica serovar Typhimurium
?
ADP-sugar + H2O
-
209944
Escherichia coli
AMP + sugar phosphate
-
209944
Escherichia coli
?
GDP-Glc + H2O
very poor substrate
209944
Salmonella enterica subsp. enterica serovar Typhimurium
GMP + Glc-1-phosphate
-
-
-
?
GDP-Man + H2O
very poor substrate
209944
Salmonella enterica subsp. enterica serovar Typhimurium
GMP + Man-1-phosphate
-
-
-
?
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5
-
no activity even in presence of Mg2+ or Co2+
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.9
-
ADP-Glc
-
Salmonella enterica subsp. enterica serovar Typhimurium
40
-
GDP-Glc
same Km for GDP-Glc and GDP-Man
Salmonella enterica subsp. enterica serovar Typhimurium
40
-
GDP-Man
same Km for GDP-Glc and GDP-Man
Salmonella enterica subsp. enterica serovar Typhimurium
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Escherichia coli
-
-
soluble
-
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Salmonella enterica subsp. enterica serovar Typhimurium
Mg2+
-
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ADP-sugar + H2O
Salmonella enterica subsp. enterica serovar Typhimurium
-
AMP + sugar phosphate
-
Salmonella enterica subsp. enterica serovar Typhimurium
?
ADP-sugar + H2O
Escherichia coli
-
AMP + sugar phosphate
-
Escherichia coli
?
Purification (Commentary) (protein specific)
Commentary
Organism
partial purification together with UDP-sugar pyrophosphatase
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ADP-sugar + H2O
-
209944
Salmonella enterica subsp. enterica serovar Typhimurium
AMP + sugar phosphate
-
209944
Salmonella enterica subsp. enterica serovar Typhimurium
?
ADP-sugar + H2O
-
209944
Escherichia coli
AMP + sugar phosphate
-
209944
Escherichia coli
?
GDP-Glc + H2O
very poor substrate
209944
Salmonella enterica subsp. enterica serovar Typhimurium
GMP + Glc-1-phosphate
-
-
-
?
GDP-Man + H2O
very poor substrate
209944
Salmonella enterica subsp. enterica serovar Typhimurium
GMP + Man-1-phosphate
-
-
-
?
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5
-
no activity even in presence of Mg2+ or Co2+
Escherichia coli
Other publictions for EC 3.6.1.21
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
697980
Moran-Zorzano
Cytoplasmic Escherichia coli A ...
Escherichia coli BL21(DE3)
FEMS Microbiol. Lett.
288
25-32
2008
1
-
-
-
-
-
-
-
4
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
657184
Moreno-Bruna
Adenosine diphosphate sugar py ...
Escherichia coli
Proc. Natl. Acad. Sci. USA
98
8128-8132
2001
-
-
1
-
-
-
6
3
-
-
2
1
-
3
-
-
1
-
-
-
1
-
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
6
-
3
-
-
2
1
-
-
-
1
-
-
1
-
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
209943
Providenzia
A pyrophosphatase from mammali ...
Bos taurus, Oryctolagus cuniculus, Rattus norvegicus
Biochim. Biophys. Acta
167
199-201
1968
-
-
-
-
-
-
6
6
-
3
-
3
-
3
-
-
3
-
-
8
-
-
18
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
6
-
3
-
3
-
-
-
3
-
8
-
-
18
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
209944
Glaser
Uridine diphosphate sugar hydr ...
Escherichia coli, Salmonella enterica subsp. enterica serovar Typhimurium
J. Biol. Chem.
242
1944-1954
1967
-
-
-
-
-
-
-
3
2
2
-
2
-
2
-
-
1
-
-
-
-
-
4
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
2
2
-
2
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
209945
Melo
Nucleotide diphosphate hexose ...
Escherichia coli, Salmonella weslaco
Biochem. Biophys. Res. Commun.
22
524-531
1966
-
-
-
-
-
-
2
-
2
2
-
2
-
2
-
-
1
-
-
-
2
-
2
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
2
2
-
2
-
-
-
1
-
-
2
-
2
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-