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Literature summary for 3.6.1.18 extracted from
- Nucleotidases in plants. III. Effect of metabolites on the enzyme hydrolyzing flavine adenine dinucleotide (FAD) from Phaseolus radiatus (1969), Arch. Biochem. Biophys., 133, 54-59.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ADP | reactivates aged enzyme, activating up to 0.01 mM | Vigna radiata var. radiata |  |
| p-chloromercuribenzoate | - |
Vigna radiata var. radiata | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | inhibitory above 0.5 mM | Vigna radiata var. radiata | |
| AMP | complete inhibition above 1 mM | Vigna radiata var. radiata | |
| ATP | non-competitive | Vigna radiata var. radiata | |
| CMP | slight inhibition | Vigna radiata var. radiata | |
| diphosphate | - |
Vigna radiata var. radiata | |
| FMN | no inhibitory effect | Vigna radiata var. radiata | |
| UMP | slight inhibition | Vigna radiata var. radiata | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
FAD | - |
Vigna radiata var. radiata | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| FAD + H2O | Vigna radiata var. radiata | - |
FMN + AMP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vigna radiata var. radiata | - |
mung bean seedlings | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Vigna radiata var. radiata |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| reversible denaturation with urea | Vigna radiata var. radiata |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 12 h, concentration below 0.2 mg/ml, 60% loss of activity, can be restored by adding ADP | Vigna radiata var. radiata |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| FAD + H2O | - |
Vigna radiata var. radiata | FMN + AMP | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 49 | - |
- |
Vigna radiata var. radiata |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.4 | - |
- |
Vigna radiata var. radiata |
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