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Literature summary for 3.6.1.17 extracted from

  • Jeyakanthan, J.; Kanaujia, S.P.; Nishida, Y.; Nakagawa, N.; Praveen, S.; Shinkai, A.; Kuramitsu, S.; Yokoyama, S.; Sekar, K.
    Free and ATP-bound structures of Ap4A hydrolase from Aquifex aeolicus V5 (2010), Acta Crystallogr. Sect. D, 66, 116-124.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells Aquifex aeolicus

Crystallization (Commentary)

Crystallization (Comment) Organism
free and ATP-bound enzyme, hanging drop vapor diffusion method, using 0.1 M Tris-HCl pH 7.6, 29% (w/v) PEG 3350, and 0.75 M NaCl (free enzyme) or 1 ml 0.1 M Tris-HCl pH 8.8, 25% (w/v) PEG 3350, and 0.2 M NaCl (ATP-bound enzyme) Aquifex aeolicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.054
-
P1,P4-bis(5'-adenosyl) tetraphosphate in 50 mM Tris-HCl, 100 mM KCl, 5 mM MgCl2, pH 7.6, at 25°C Aquifex aeolicus
0.089
-
P1,P6-bis(5'-adenosyl) hexaphosphate in 50 mM Tris-HCl, 100 mM KCl, 5 mM MgCl2, pH 7.6, at 25°C Aquifex aeolicus
0.183
-
P1,P5-bis(5'-adenosyl) pentaphosphate in 50 mM Tris-HCl, 100 mM KCl, 5 mM MgCl2, pH 7.6, at 25°C Aquifex aeolicus

Organism

Organism UniProt Comment Textmining
Aquifex aeolicus O66548
-
-

Purification (Commentary)

Purification (Comment) Organism
Toyopearl SuperQ-650M column chromatography, Resource Q column chromatography, Bio-Scale CHT2-I column chromatography, and Superdex 75 gel filtration Aquifex aeolicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O best substrate Aquifex aeolicus ATP + AMP
-
?
P1,P5-bis(5'-adenosyl) pentaphosphate + H2O
-
Aquifex aeolicus ATP + ADP
-
?
P1,P6-bis(5'-adenosyl) hexaphosphate + H2O
-
Aquifex aeolicus ATP
-
?

Synonyms

Synonyms Comment Organism
Ap4A hydrolase
-
Aquifex aeolicus
asymmetric Ap4A hydrolase
-
Aquifex aeolicus
asymmetric diadenosine tetraphosphate hydrolase
-
Aquifex aeolicus
ATP-producing Ap4A hydrolase
-
Aquifex aeolicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.2
-
P1,P6-bis(5'-adenosyl) hexaphosphate in 50 mM Tris-HCl, 100 mM KCl, 5 mM MgCl2, pH 7.6, at 25°C Aquifex aeolicus
1.4
-
P1,P5-bis(5'-adenosyl) pentaphosphate in 50 mM Tris-HCl, 100 mM KCl, 5 mM MgCl2, pH 7.6, at 25°C Aquifex aeolicus
2.5
-
P1,P4-bis(5'-adenosyl) tetraphosphate in 50 mM Tris-HCl, 100 mM KCl, 5 mM MgCl2, pH 7.6, at 25°C Aquifex aeolicus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.2
-
P1,P6-bis(5'-adenosyl) hexaphosphate in 50 mM Tris-HCl, 100 mM KCl, 5 mM MgCl2, pH 7.6, at 25°C Aquifex aeolicus
7.6
-
P1,P5-bis(5'-adenosyl) pentaphosphate in 50 mM Tris-HCl, 100 mM KCl, 5 mM MgCl2, pH 7.6, at 25°C Aquifex aeolicus
46
-
P1,P4-bis(5'-adenosyl) tetraphosphate in 50 mM Tris-HCl, 100 mM KCl, 5 mM MgCl2, pH 7.6, at 25°C Aquifex aeolicus