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Literature summary for 3.6.1.15 extracted from

  • Han, K.R.; Lee, J.H.; Kotiguda, G.G.; Jung, K.H.; Chung, M.S.; Kang, S.; Hwang, S.; Kim, K.H.
    Nucleotide triphosphatase and RNA chaperone activities of murine norovirus NS3 (2018), J. Gen. Virol., 99, 1482-1493 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.1.15

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of C-terminally His-tagged enzyme in Escherichia coli in inclusion bodies, recombinant expression of MBP-tagged enzyme in Escherichia coli Murine norovirus

Protein Variants

Protein Variants Comment Organism
D213A site-directed mutagenesis, mutant shows significantly reduced NTPase activity compared to the wild-type NS3 protein Murine norovirus
K169A site-directed mutagenesis, the mutant shows significantly reduced NTPase activity compared to the wild-type NS3 protein Murine norovirus
additional information site-directed mutagenesis of the conserved NTPase motifs in NS3 blocking enzyme activity and viral replication in cells Murine norovirus
N260A site-directed mutagenesis, mutant shows significantly reduced NTPase activity compared to the wild-type NS3 protein Murine norovirus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ dependent on, 4fold activation at 5 mM Murine norovirus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1200000
-
 recombinant enzyme oligomer, native PAGE Murine norovirus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O Murine norovirus
-
 ADP + phosphate
-
 ?
CTP + H2O Murine norovirus
-
 CDP + phosphate
-
 ?
GTP + H2O Murine norovirus
-
 GDP + phosphate
-
 ?
UTP + H2O Murine norovirus
-
 UDP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Murine norovirus
-
 MNV
-

Purification (Commentary)

Purification (Comment) Organism
recombinant MBP-tagged enzyme from Escherichia coli by amylose affinity chromatography and gel filtration. When the MBP-NS3 is treated with TEV protease to remove its MBP tag, the free NS3 becomes unstable and aggregates Murine norovirus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
 Murine norovirus ADP + phosphate
-
 ?
CTP + H2O
-
 Murine norovirus CDP + phosphate
-
 ?
GTP + H2O
-
 Murine norovirus GDP + phosphate
-
 ?
additional information ATP and GTP are preferred over CTP and UTP for hydrolysis by MNV NS3, with ATP being the most favoured substrate. The MNV NS3 has an NTPase-independent RNA chaperone-like activity. NS3 destabilizes double-stranded RNA in the presence of Mg2+ or Mn2+ in an NTP-independent manner Murine norovirus ?
-
-
UTP + H2O
-
 Murine norovirus UDP + phosphate
-
 ?

Subunits

Subunits Comment Organism
More MNV NS3 has three domains: the N-terminal domain (NTD, 1-105) with a linker (106-129), the core domain (130-285) and the C-terminal domain (CTD, 290-364) Murine norovirus

Synonyms

Synonyms Comment Organism
Mg2+-dependent NTPase
-
 Murine norovirus
NS3
-
 Murine norovirus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Murine norovirus

General Information

General Information Comment Organism
malfunction mutation of the conserved NTPase motifs blocks enzyme activity and viral replication in cells. The RNA destabilization activity is not affected by mutagenesis of the conserved motifs of NTPase Murine norovirus
additional information MNV NS3 has three domains: the N-terminal domain (NTD, 1-105) with a linker (106-129), the core domain (130-285) and the C-terminal domain (CTD, 290-364). The conserved motifs are crucial to the NTPase activity of NS3 Murine norovirus