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Literature summary for 3.6.1.15 extracted from
- Purification and characteristics of functional properties of soluble nucleoside triphosphatase (apyrase) from bovine brain (2008), Biochemistry (Moscow), 73, 1047-1052.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-oxoglutarate | stimulating in physiological concentrations, 148% relative activity at a concentration of 0.5 mM, 128% relative activity at a concentration of 0.05 mM | Bos taurus |  |
| acetyl-CoA | stimulating in physiological concentrations, 148% relative activity at a concentration of 0.5 mM, 127% relative activity at a concentration of 0.05 mM | Bos taurus | |
| D-fructose 1,6-bisphosphate | stimulating in physiological concentrations, 155% relative activity at a concentration of 0.5 mM, 126% relative activity at a concentration of 0.05 mM | Bos taurus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| propionate | 39% relative activity at a concentration of 0.5 mM, 63% relative activity at a concentration of 0.05 mM | Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.025 | - |
ITP | in presence of 10 mM Mg2+ | Bos taurus | |
| 0.041 | - |
GTP | in presence of 10 mM Mg2+ | Bos taurus | |
| 0.086 | - |
UTP | in presence of 10 mM Mg2+ | Bos taurus | |
| 0.15 | - |
CTP | in presence of 10 mM Mg2+ | Bos taurus | |
| 0.26 | - |
ATP | in presence of 10 mM Mg2+ | Bos taurus | |
| 0.5 | - |
CDP | in presence of 10 mM Mg2+ | Bos taurus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Bos taurus | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | 5 mM, unable to stimulate enzyme activity | Bos taurus | |
| Ca2+ | 5 mM, 52% relative enzyme activity in presence of | Bos taurus | |
| Co2+ | 5 mM, 60% relative enzyme activity in presence of | Bos taurus | |
| Cu2+ | 5 mM, 46% relative enzyme activity in presence of | Bos taurus | |
| Mg2+ | 5 mM, 100% relative enzyme activity in presence of | Bos taurus | |
| Mn2+ | 5 mM, 73% relative enzyme activity in presence of | Bos taurus | |
| Zn2+ | 5 mM, 34% relative enzyme activity in presence of | Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58600 | - |
gel filtration, at 4°C in 20 mM Tris-HCl, 0.1 M NaCl, pH 7.5 | Bos taurus |
| 60200 | - |
SDS-PAGE | Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + H2O | Bos taurus | 0.5 mM, 25% relative activity towards ADP compared to CTP, no activity towards AMP | AMP + phosphate | - |
? | |
| ATP + H2O | Bos taurus | 0.5 mM, 32% relative activity compared to CTP | ADP + phosphate | - |
? | |
| CDP + H2O | Bos taurus | 0.5 mM, 41% relative activity towards CDP, no activity towards CMP | CMP + phosphate | - |
? | |
| CTP + H2O | Bos taurus | 0.5 mM, 100% relative activity | CDP + phosphate | - |
? | |
| GDP + H2O | Bos taurus | 0.5 mM, 19% relative activity towards GDP compared to CTP, no activity towards GMP | GMP + phosphate | - |
? | |
| GTP + H2O | Bos taurus | 0.5 mM, 30% relative activity compared to CTP | GDP + phosphate | - |
? | |
| IDP + H2O | Bos taurus | 0.5 mM, 41% relative activity towards IDP compared to CTP, 5.3% relative activity towards IMP compared to CTP | IMP + phosphate | - |
? | |
| ITP + H2O | Bos taurus | 0.5 mM, 58% relative activity compared to CTP | IDP + phosphate | - |
? | |
| UDP + H2O | Bos taurus | 0.5 mM, 32% relative activity compared to CTP, 8.2% relative activity towards UMP compared to CTP | UMP + phosphate | - |
? | |
| UTP + H2O | Bos taurus | 0.5 mM, 66% relative activity compared to CTP | UDP + phosphate | - |
? | |
| XTP + H2O | Bos taurus | 0.5 mM, 40% relative activity compared to CTP | XDP + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| gel filtration, SDS-PAGE | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | purified from | Bos taurus | - |
| heart | expression in | Bos taurus | - |
| kidney | expression in | Bos taurus | - |
| liver | expression in | Bos taurus | - |
| lung | expression in | Bos taurus | - |
| small intestine | expression in | Bos taurus | - |
| spleen | expression in | Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
dependence on bivalent cations and broad substrate specificity towards nucleoside-5'-tri- and -diphosphates reveal similarities to apyrases | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + H2O | 0.5 mM, 25% relative activity towards ADP compared to CTP, no activity towards AMP | Bos taurus | AMP + phosphate | - |
? | |
| ATP + H2O | 0.5 mM, 32% relative activity compared to CTP | Bos taurus | ADP + phosphate | - |
? | |
| CDP + H2O | 0.5 mM, 41% relative activity towards CDP, no activity towards CMP | Bos taurus | CMP + phosphate | - |
? | |
| CDP + H2O | 0.5 mM, 41% relative activity towards CDP compared to CTP, no activity towards CMP | Bos taurus | CMP + phosphate | - |
? | |
| CTP + H2O | 0.5 mM, 100% relative activity | Bos taurus | CDP + phosphate | - |
? | |
| GDP + H2O | 0.5 mM, 19% relative activity towards GDP compared to CTP, no activity towards GMP | Bos taurus | GMP + phosphate | - |
? | |
| GTP + H2O | 0.5 mM, 30% relative activity compared to CTP | Bos taurus | GDP + phosphate | - |
? | |
| IDP + H2O | 0.5 mM, 41% relative activity towards IDP compared to CTP, 5.3% relative activity towards IMP compared to CTP | Bos taurus | IMP + phosphate | - |
? | |
| IDP + H2O | 0.5 mM, 41% relative activity towards IDP, 5.3% relative activity towards IMP compared to CTP | Bos taurus | IMP + phosphate | - |
? | |
| ITP + H2O | 0.5 mM, 58% relative activity compared to CTP | Bos taurus | IDP + phosphate | - |
? | |
| UDP + H2O | 0.5 mM, 32% relative activity compared to CTP, 8.2% relative activity towards UMP compared to CTP | Bos taurus | UMP + phosphate | - |
? | |
| UDP + H2O | 0.5 mM, 32% relative activity, 8.2% relative activity towards UMP compared to CTP | Bos taurus | UMP + phosphate | - |
? | |
| UTP + H2O | 0.5 mM, 66% relative activity compared to CTP | Bos taurus | UDP + phosphate | - |
? | |
| XTP + H2O | 0.5 mM, 40% relative activity compared to CTP | Bos taurus | XDP + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 60200, SDS-PAGE | Bos taurus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| nucleoside triphosphatase | - |
Bos taurus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Bos taurus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8.5 | pH 6.0: about 55% of maximal activity, pH 8.5: about 55% of maximal activity | Bos taurus |
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