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Literature summary for 3.6.1.15 extracted from
- Functional role of conserved residues in the characteristic secretion NTPase motifs of the Pseudomonas aeruginosa type IV pilus motor proteins PilB, PilT and PilU (2008), Microbiology, 154, 114-126.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| genes pilB, pilT and pilU, DNA and amino acid sequence determination and anaylsis, expression of N-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli strain Bl21 | Pseudomonas aeruginosa |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D160N | site-directed mutagenesis, mutation of the Asp Box motif residue in PilT | Pseudomonas aeruginosa |
| E159Q | site-directed mutagenesis, mutation of the Asp Box motif residue in PilT | Pseudomonas aeruginosa |
| E163Q | site-directed mutagenesis, mutation of the Asp Box motif residue in PilT | Pseudomonas aeruginosa |
| E204Q | site-directed mutagenesis, mutation of the Walker B motif residue in PilT | Pseudomonas aeruginosa |
| G135S | site-directed mutagenesis, mutation of the Walker A motif residue in PilT | Pseudomonas aeruginosa |
| H222A | site-directed mutagenesis, mutation of the His Box motif residue in PilT | Pseudomonas aeruginosa |
| H229A | site-directed mutagenesis, mutation of the His Box motif residue in PilT | Pseudomonas aeruginosa |
| additional information | mutation of conserved Walker A or Walker B residues in any of the three ATPases abrogates twitching motility, and for the Walker A mutant of PilT causes loss of polar localization, construction of diverse mutants of the type IV pilus motor proteins, overview | Pseudomonas aeruginosa |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Pseudomonas aeruginosa |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
genes pilB, pilT and pilU | - |
| Pseudomonas aeruginosa PAK | - |
genes pilB, pilT and pilU | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain Bl21 by nickel affinity chromatography | Pseudomonas aeruginosa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
activity of mutant enzymes, overview | Pseudomonas aeruginosa |
| 0.00008 | - |
PilU | Pseudomonas aeruginosa |
| 0.00012 | - |
PilT | Pseudomonas aeruginosa |
| 0.00018 | - |
PilB | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | recombinant His-tagged PilB, PilT, and PilU show ATPase activity in vitro with requirement for three invariant acidic residues in the Asp Box motif, and for two invariant His residues in the His Box motif to varying extents, overview | Pseudomonas aeruginosa | ADP + phosphate | - |
? | |
| ATP + H2O | recombinant His-tagged PilB, PilT, and PilU show ATPase activity in vitro with requirement for three invariant acidic residues in the Asp Box motif, and for two invariant His residues in the His Box motif to varying extents, overview | Pseudomonas aeruginosa PAK | ADP + phosphate | - |
? | |
| NTP + H2O | - |
Pseudomonas aeruginosa | NDP + phosphate | - |
? | |
| NTP + H2O | - |
Pseudomonas aeruginosa PAK | NDP + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | the type IV pilus motor proteins contain conserved residues in the Walker A, Walker B, and Asp Box and His Box motifs characteristic of secretion NTPases, overview | Pseudomonas aeruginosa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | PilB and PilT are bipolar proteins belonging to the secretion NTPase superfamily | Pseudomonas aeruginosa |
| PilB | - |
Pseudomonas aeruginosa |
| PilT | - |
Pseudomonas aeruginosa |
| PilU | - |
Pseudomonas aeruginosa |
| T4P motor protein | - |
Pseudomonas aeruginosa |
| type IV pilus motor protein | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 24 | - |
assay at | Pseudomonas aeruginosa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Pseudomonas aeruginosa |
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