Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression of wild-type and mutant enzymes in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
E113T | site-directed mutagenesis, the mutation in the NTP binding site has a significant effect on the hydrolysis rate of the purine nucleotides, but has only a small effect on the protein structure | Homo sapiens |
G115H | site-directed mutagenesis, the mutation in the NTP binding site has a significant effect on the hydrolysis rate of the purine nucleotides, but has only a small effect on the protein structure | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
GTP | pH 7.0, 22°C, recombinant wild-type enzyme | Homo sapiens | |
0.005 | - |
ATP | pH 7.0, 22°C, recombinant wild-type enzyme | Homo sapiens | |
0.005 | - |
UTP | pH 7.0, 22°C, recombinant wild-type enzyme | Homo sapiens | |
0.006 | - |
CTP | pH 7.0, 22°C, recombinant wild-type enzyme | Homo sapiens | |
0.007 | - |
TTP | pH 7.0, 22°C, recombinant wild-type enzyme | Homo sapiens | |
0.046 | - |
ADP | pH 7.0, 22°C, recombinant wild-type enzyme | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20500 | - |
x * 20500, recombinant enzyme, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + H2O | Homo sapiens | - |
AMP + phosphate | - |
? | |
ATP + H2O | Homo sapiens | - |
ADP + phosphate | - |
? | |
CTP + H2O | Homo sapiens | - |
CDP + phosphate | - |
? | |
GTP + H2O | Homo sapiens | - |
GDP + phosphate | - |
? | |
TTP + H2O | Homo sapiens | - |
TDP + phosphate | - |
? | |
UTP + H2O | Homo sapiens | - |
UDP + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from Escherichia coli by cation exchange chromatography and gel filtration to homogeneity | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + H2O | - |
Homo sapiens | AMP + phosphate | - |
? | |
ATP + H2O | - |
Homo sapiens | ADP + phosphate | - |
? | |
ATP + H2O | HCR-NTPase contains both residues E113 and G115, and has nearly identical hydrolysis rates for ATP and GTP | Homo sapiens | ADP + phosphate | - |
? | |
CTP + H2O | - |
Homo sapiens | CDP + phosphate | - |
? | |
GTP + H2O | - |
Homo sapiens | GDP + phosphate | - |
? | |
GTP + H2O | HCR-NTPase contains both residues E113 and G115, and has nearly identical hydrolysis rates for ATP and GTP | Homo sapiens | GDP + phosphate | - |
? | |
additional information | HCR-NTPase is a non-selective NTPase with only slightly higher activity for purine nucleoside triphosphates, and with a comparatively low rate of hydrolysis, structural basis of HCR-NTPase activity, overview | Homo sapiens | ? | - |
? | |
TTP + H2O | - |
Homo sapiens | TDP + phosphate | - |
? | |
UTP + H2O | - |
Homo sapiens | UDP + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 20500, recombinant enzyme, SDS-PAGE | Homo sapiens |
More | three-dimensional structure determination by NMR and MALDI-TOF mass spectrometry, and structure analysis. The structure of the protein in its complex with ATPgammaS forms a three-layered alpha/beta sandwich, with a central nine-stranded beta-sheet surrounded by five alpha-helices. Sequence and three-dimensional structure comparisons with AAA+ ATPases revealed the presence of Walker A, GPPGVGKT, and Walker B, VCVIDEIG, motifs, overview | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
HCR-NTPase | - |
Homo sapiens |
human cancer-related nucleoside triphosphatase | - |
Homo sapiens |
non-specific nucleoside triphosphatase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0051 | - |
ADP | pH 7.0, 22°C, recombinant wild-type enzyme | Homo sapiens | |
0.007 | - |
UTP | pH 7.0, 22°C, recombinant wild-type enzyme | Homo sapiens | |
0.0073 | - |
CTP | pH 7.0, 22°C, recombinant wild-type enzyme | Homo sapiens | |
0.0074 | - |
TTP | pH 7.0, 22°C, recombinant wild-type enzyme | Homo sapiens | |
0.0076 | - |
GTP | pH 7.0, 22°C, recombinant wild-type enzyme | Homo sapiens | |
0.0086 | - |
ATP | pH 7.0, 22°C, recombinant wild-type enzyme | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Homo sapiens |