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Literature summary for 3.6.1.15 extracted from
- Identification and characterization of the NTPase activity of classical swine fever virus (CSFV) nonstructural protein 3 (NS3) expressed in bacteria (2007), Arch. Virol., 152, 1565-1573.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Poly(U) | the activation by poly(U) is inhibited by K+ | Classical swine fever virus |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, functional expression of His-tagged full-length viral NS3 protein, i.e. NS3FL, and its N-terminal truncated version ntNS3 in Escherichia coli | Classical swine fever virus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A354V | site-directed mutagenesis of the motif III residue does not affect the enzyme activity and kinetics | Classical swine fever virus |
| K232A | site-directed mutagenesis of the conserved motif I residue leads to inactivation of the mutant | Classical swine fever virus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Classical swine fever virus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required, activates | Classical swine fever virus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
x * 60000, recombinant full-length NS3 protein, SDS-PAGE | Classical swine fever virus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | Classical swine fever virus | - |
ADP + phosphate | - |
? | |
| ATP + H2O | Classical swine fever virus CSFV | - |
ADP + phosphate | - |
? | |
| CTP + H2O | Classical swine fever virus | - |
CDP + phosphate | - |
? | |
| dATP + H2O | Classical swine fever virus | - |
dADP + phosphate | - |
? | |
| dCTP + H2O | Classical swine fever virus | - |
dCDP + phosphate | - |
? | |
| dGTP + H2O | Classical swine fever virus | - |
dGDP + phosphate | - |
? | |
| dUTP + H2O | Classical swine fever virus | - |
dUDP + phosphate | - |
? | |
| GTP + H2O | Classical swine fever virus | - |
GDP + phosphate | - |
? | |
| GTP + H2O | Classical swine fever virus CSFV | - |
GDP + phosphate | - |
? | |
| additional information | Classical swine fever virus | the viral NS3 protein, i.e. NS3FL, and its N-terminal truncated version ntNS3, recombinantly expressed in Escherichia coli, contain specific polynucleotide-stimulated NTPase acitivity, the enzyme activity is essential for viral replication | ? | - |
? | |
| additional information | Classical swine fever virus CSFV | the viral NS3 protein, i.e. NS3FL, and its N-terminal truncated version ntNS3, recombinantly expressed in Escherichia coli, contain specific polynucleotide-stimulated NTPase acitivity, the enzyme activity is essential for viral replication | ? | - |
? | |
| UTP + H2O | Classical swine fever virus | - |
UDP + phosphate | - |
? | |
| UTP + H2O | Classical swine fever virus CSFV | - |
UDP + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Classical swine fever virus | - |
CSFV | - |
| Classical swine fever virus CSFV | - |
CSFV | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged full-length viral NS3 protein and N-terminal truncated version ntNS3 from Escherichia coli by nickel affinity chromatography and dialysis | Classical swine fever virus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Classical swine fever virus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | - |
Classical swine fever virus | ADP + phosphate | - |
? | |
| ATP + H2O | - |
Classical swine fever virus CSFV | ADP + phosphate | - |
? | |
| CTP + H2O | - |
Classical swine fever virus | CDP + phosphate | - |
? | |
| dATP + H2O | - |
Classical swine fever virus | dADP + phosphate | - |
? | |
| dCTP + H2O | - |
Classical swine fever virus | dCDP + phosphate | - |
? | |
| dGTP + H2O | - |
Classical swine fever virus | dGDP + phosphate | - |
? | |
| dUTP + H2O | - |
Classical swine fever virus | dUDP + phosphate | - |
? | |
| GTP + H2O | - |
Classical swine fever virus | GDP + phosphate | - |
? | |
| GTP + H2O | - |
Classical swine fever virus CSFV | GDP + phosphate | - |
? | |
| additional information | the viral NS3 protein, i.e. NS3FL, and its N-terminal truncated version ntNS3, recombinantly expressed in Escherichia coli, contain specific polynucleotide-stimulated NTPase acitivity, the enzyme activity is essential for viral replication | Classical swine fever virus | ? | - |
? | |
| additional information | the conserved motif I, GXGK232T, is required for NTPase activity of the viral NS3 protein, while motif III is not | Classical swine fever virus | ? | - |
? | |
| additional information | the viral NS3 protein, i.e. NS3FL, and its N-terminal truncated version ntNS3, recombinantly expressed in Escherichia coli, contain specific polynucleotide-stimulated NTPase acitivity, the enzyme activity is essential for viral replication | Classical swine fever virus CSFV | ? | - |
? | |
| additional information | the conserved motif I, GXGK232T, is required for NTPase activity of the viral NS3 protein, while motif III is not | Classical swine fever virus CSFV | ? | - |
? | |
| UTP + H2O | - |
Classical swine fever virus | UDP + phosphate | - |
? | |
| UTP + H2O | - |
Classical swine fever virus CSFV | UDP + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 60000, recombinant full-length NS3 protein, SDS-PAGE | Classical swine fever virus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | the enzyme is a nonstructural protein 3, NS3 of family Flaviviridae members | Classical swine fever virus |
| NS3 protein | - |
Classical swine fever virus |
| NS3FL | - |
Classical swine fever virus |
| NTPase | - |
Classical swine fever virus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Classical swine fever virus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 82 | - |
dUTP | pH 6.5, 37°C, recombinant truncated protein ntNS3, in absence of poly(U) | Classical swine fever virus | |
| 114 | - |
UTP | pH 6.5, 37°C, recombinant truncated protein ntNS3, in absence of poly(U) | Classical swine fever virus | |
| 135 | - |
dCTP | pH 6.5, 37°C, recombinant truncated protein ntNS3, in absence of poly(U) | Classical swine fever virus | |
| 136 | - |
dGTP | pH 6.5, 37°C, recombinant truncated protein ntNS3, in absence of poly(U) | Classical swine fever virus | |
| 155 | - |
ATP | pH 6.5, 37°C, recombinant truncated protein ntNS3, in absence of poly(U) | Classical swine fever virus | |
| 208 | - |
dATP | pH 6.5, 37°C, recombinant truncated protein ntNS3, in absence of poly(U) | Classical swine fever virus | |
| 220 | - |
CTP | pH 6.5, 37°C, recombinant truncated protein ntNS3, in absence of poly(U) | Classical swine fever virus | |
| 400 | - |
GTP | pH 6.5, 37°C, recombinant truncated protein ntNS3, in absence of poly(U) | Classical swine fever virus | |
| 1875 | - |
dUTP | pH 6.5, 37°C, recombinant truncated protein ntNS3, in presence of poly(U) | Classical swine fever virus | |
| 2162 | - |
UTP | pH 6.5, 37°C, recombinant truncated protein ntNS3, in presence of poly(U) | Classical swine fever virus | |
| 2286 | - |
CTP | pH 6.5, 37°C, recombinant truncated protein ntNS3, in presence of poly(U) | Classical swine fever virus | |
| 2500 | - |
dGTP | pH 6.5, 37°C, recombinant truncated protein ntNS3, in presence of poly(U) | Classical swine fever virus | |
| 2963 | - |
GTP | pH 6.5, 37°C, recombinant truncated protein ntNS3, in presence of poly(U) | Classical swine fever virus | |
| 3150 | - |
dATP | pH 6.5, 37°C, recombinant truncated protein ntNS3, in presence of poly(U) | Classical swine fever virus | |
| 4384 | - |
ATP | pH 6.5, 37°C, recombinant truncated protein ntNS3, in presence of poly(U) | Classical swine fever virus | |
| 5096 | - |
dCTP | pH 6.5, 37°C, recombinant truncated protein ntNS3, in presence of poly(U) | Classical swine fever virus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | broad range optimum, pH profile | Classical swine fever virus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9 | pH profile | Classical swine fever virus |
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