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Literature summary for 3.6.1.15 extracted from

  • Rakitina, D.V.; Kantidze, O.L.; Leshchiner, A.D.; Solovyev, A.G.; Novikov, V.K.; Morozov, S.Y.; Kalinina, N.O.
    Coat proteins of two filamentous plant viruses display NTPase activity in vitro (2005), FEBS Lett., 579, 4955-4960.
    View publication on PubMed
Search for more literature for 3.6.1.15

Cloned(Commentary)

Cloned (Comment) Organism
functional overexpression of the His6-tagged coat protein in Escherichia coli potato virus A
functional overexpression of the His6-tagged coat protein in Escherichia coli potato virus X

Protein Variants

Protein Variants Comment Organism
additional information virus C-terminal regions deletion diminishes the ATPase activity potato virus X

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 potato virus A
EDTA
-
 potato virus X
rNTP ribotrinucleotides inhibit the activity with gammaATP potato virus A
rNTP ribotrinucleotides inhibit the activity with gammaATP potato virus X

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates slightly, much less efficient than Mg2+ potato virus A
Ca2+ activates, less efficient than Mg2+ potato virus X
Cu2+ activates slightly, much less efficient than Mg2+ potato virus A
Cu2+ activates slightly, much less efficient than Mg2+ potato virus X
Mg2+ best metal ion for enzyme activation potato virus A
Mg2+ best metal ion for enzyme activation potato virus X
Mn2+ activates, less efficient than Mg2+ potato virus A
Mn2+ activates, less efficient than Mg2+ potato virus X
additional information the enzyme is dependent on divalent cations potato virus A
additional information the enzyme is dependent on divalent cations potato virus X
Zn2+ activates slightly, much less efficient than Mg2+ potato virus A
Zn2+ activates slightly, much less efficient than Mg2+ potato virus X

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O potato virus A
-
 ADP + phosphate
-
 ?
ATP + H2O potato virus X
-
 ADP + phosphate
-
 ?
additional information potato virus A the coat protein of the plant virus exhibits NTPase activity, the coat proteins are involved in different stages of the viral life cycle such as virion assembyl, replication, movement, vector transmission, and regulation of host defense response ?
-
 ?
additional information potato virus X the coat protein of the plant virus exhibits NTPase activity, the coat proteins are involved in different stages of the viral life cycle such as virion assembyl, replication, movement, vector transmission, and regulation of host defense response ?
-
 ?

Organism

Organism UniProt Comment Textmining
no activity in tobacco mosaic virus
-
-
-
potato virus A
-
 PVA, a poteyvirus
-
potato virus X
-
 PVX, a potexvirus
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged coat protein from Escherichia coli by nickel affinity chromatogryphy potato virus A
recombinant His6-tagged coat protein from Escherichia coli by nickel affinity chromatogryphy potato virus X

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 potato virus A
additional information
-
-
 potato virus X

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
 potato virus A ADP + phosphate
-
 ?
ATP + H2O
-
 potato virus X ADP + phosphate
-
 ?
additional information the coat protein of the plant virus exhibits NTPase activity, the coat proteins are involved in different stages of the viral life cycle such as virion assembyl, replication, movement, vector transmission, and regulation of host defense response potato virus A ?
-
 ?
additional information the coat protein of the plant virus exhibits NTPase activity, the coat proteins are involved in different stages of the viral life cycle such as virion assembyl, replication, movement, vector transmission, and regulation of host defense response potato virus X ?
-
 ?
additional information the enzyme hydrolyzes ribonucleotides and deoxyribonucleotides potato virus X ?
-
 ?
UTP + H2O recombinant enzyme potato virus A UDP + phosphate
-
 ?
UTP + H2O recombinant enzyme potato virus X UDP + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
NTPase
-
 potato virus A
NTPase
-
 potato virus X
PVA coat protein
-
 potato virus A
PVA CP
-
 potato virus A
PVx coat protein
-
 potato virus X
PVX CP
-
 potato virus X