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Literature summary for 3.6.1.14 extracted from

  • Guranowski, A.; Starzynska, E.; Brown, P.; Blackburn, G.M.
    Adenosine 5'-tetraphosphate phosphohydrolase from yellow lupin seeds: purification to homogeneity and some properties (1997), Biochem. J., 328, 257-262.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.1.14

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
 Lupinus luteus
F-
-
 Lupinus luteus
Zn2+
-
 Lupinus luteus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
 adenosine 5'-tetraphosphate
-
 Lupinus luteus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ divalent metal ion, Mg2+, Co2+, Ni2+ or Mn2+, required Lupinus luteus
Mg2+ divalent metal ion, Mg2+, Co2+, Ni2+ or Mn2+ required Lupinus luteus
Mn2+ divalent metal ion, Mg2+, Co2+, Ni2+ or Mn2+ required Lupinus luteus
Ni2+ divalent metal ion, Mg2+, Co2+, Ni2+ or Mn2+ required Lupinus luteus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
 gel filtration Lupinus luteus

Organism

Organism UniProt Comment Textmining
Lupinus luteus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Lupinus luteus

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
 Lupinus luteus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
20.83
-
-
 Lupinus luteus

Storage Stability

Storage Stability Organism
-20°C, 6 months, no loss of activity Lupinus luteus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenosine 5'-pentaphosphate + H2O hydrolyzed 18fold more slowly than adenosine 5'-tetraphosphate Lupinus luteus ?
-
 ?
adenosine 5'-pentaphosphate + H2O hydrolyzed at 1/200 the rate of adenosine 5'-tetraphosphate Lupinus luteus ?
-
 ?
adenosine 5'-tetraphosphate + H2O
-
 Lupinus luteus ATP + phosphate
-
 ?
guanosine tetraphosphate + H2O
-
 Lupinus luteus GTP + phosphate
-
 ?
tripolyphosphate + H2O
-
 Lupinus luteus diphosphate + phosphate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 25000, SDS-PAGE Lupinus luteus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
-
 Lupinus luteus

pH Range

pH Minimum pH Maximum Comment Organism
7.6 9.4 50% of maximal activity at pH 7.6 and 9.4 Lupinus luteus

pH Stability

pH Stability pH Stability Maximum Comment Organism
additional information
-
 enzyme is stable at the extremes of pH for at least 30 min Lupinus luteus