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Literature summary for 3.6.1.14 extracted from

  • Small, G.D.; Cooper, C.
    Purification and properties of nucleoside tetraphosphate hydrolase from rabbit muscle (1966), Biochemistry, 5, 14-26.
    View publication on PubMed
Search for more literature for 3.6.1.14

Inhibitors

Inhibitors Comment Organism Structure
N-ethylmaleimide
-
 Oryctolagus cuniculus
p-hydroxymercuribenzoate
-
 Oryctolagus cuniculus
tripolyphosphate competitive inhibitor of adenosine tetraphosphate Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0027
-
 adenosine 5'-tetraphosphate
-
 Oryctolagus cuniculus
0.0034
-
 inosine tetraphosphate
-
 Oryctolagus cuniculus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ divalent metal ion required, the order of decreasing effectiveness for hydrolysis of adenosine 5'-tetraphosphate is: Co2+, Fe2+, Mg2+, Ni2+, Mn2+. For tripolyphosphate hydrolysis the order is: Ni2+, Co2+, Fe2+, Mg2+, Mn2+ Oryctolagus cuniculus
Fe2+ divalent metal ion required, the order of decreasing effectiveness for hydrolysis of adenosine 5'-tetraphosphate is: Co2+, Fe2+, Mg2+, Ni2+, Mn2+. For tripolyphosphate hydrolysis the order is: Ni2+, Co2+, Fe2+, Mg2+, Mn2+ Oryctolagus cuniculus
Mg2+ divalent metal ion required, the order of decreasing effectiveness for hydrolysis of adenosine tetraphosphate is: Co2+, Fe2+, Mg2+, Ni2+, Mn2+. For tripolyphosphate hydrolysis the order is: Ni2+, Co2+, Fe2+, Mg2+, Mn2+ Oryctolagus cuniculus
Mn2+ divalent metal ion required, the order of decreasing effectiveness for hydrolysis of adenosine tetraphosphate is: Co2+, Fe2+, Mg2+, Ni2+, Mn2+. For tripolyphosphate hydrolysis the order is: Ni2+, Co2+, Fe2+, Mg2+, Mn2+ Oryctolagus cuniculus
Ni2+ divalent metal ion required, the order of decreasing effectiveness for hydrolysis of adenosine tetraphosphate is: Co2+, Fe2+, Mg2+, Ni2+, Mn2+. For tripolyphosphate hydrolysis the order is: Ni2+, Co2+, Fe2+, Mg2+, Mn2+ Oryctolagus cuniculus

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
heart
-
 Oryctolagus cuniculus
-
kidney
-
 Oryctolagus cuniculus
-
liver
-
 Oryctolagus cuniculus
-
muscle
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenosine 5'-tetraphosphate + H2O
-
 Oryctolagus cuniculus ATP + phosphate
-
 ?
inosine tetraphosphate + H2O
-
 Oryctolagus cuniculus ITP + phosphate
-
 ?
tripolyphosphate + H2O
-
 Oryctolagus cuniculus diphosphate + phosphate
-
 ?