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Literature summary for 3.6.1.14 extracted from

  • Guranowski, A.; Starzynska, E.; Barnes, L.D.; Robinson, A.K.; Liu, S.
    Adenosine 5'-tetraphosphate phosphohydrolase activity is an inherent property of soluble exopolyphosphatase from yeast Saccharomyces cerevisiae (1998), Biochim. Biophys. Acta, 1380, 232-238.
    View publication on PubMed
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Inhibitors

Inhibitors Comment Organism Structure
Ammonium molybdate
-
 Saccharomyces cerevisiae
sodium o-vanadate
-
 Saccharomyces cerevisiae
Zn2+ ZnCl2 Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
 adenosine 5'-tetraphosphate pH 4.75, 50 mM sodium acetate, 5 mM CoCl2 Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ divalent cations activate hydrolysis of adenosine 5'-tetraphosphate in the order of decreasing effectiveness: Co2+, Mn2+, Mg2+, Ni2+ Saccharomyces cerevisiae
Mg2+ divalent cations activate hydrolysis of adenosine 5'-tetraphosphate in the order of decreasing effectiveness: Co2+, Mn2+, Mg2+, Ni2+ Saccharomyces cerevisiae
Mn2+ divalent cations activate hydrolysis of adenosine 5'-tetraphosphate in the order of decreasing effectiveness: Co2+, Mn2+, Mg2+, Ni2+ Saccharomyces cerevisiae
Ni2+ divalent cations activate hydrolysis of adenosine 5'-tetraphosphate in the order of decreasing effectiveness: Co2+, Mn2+, Mg2+, Ni2+ Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 x * 45000, SDS-PAGE Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenosine 5'-pentaphosphate + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
adenosine 5'-tetraphosphate + H2O
-
 Saccharomyces cerevisiae ATP + phosphate
-
 ?
guanosine tetraphosphate + H2O
-
 Saccharomyces cerevisiae GTP + phosphate
-
 ?
inosine tetraphosphate + H2O
-
 Saccharomyces cerevisiae ITP + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 45000, SDS-PAGE Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
40
-
 adenosine-5'-pentaphosphate
-
 Saccharomyces cerevisiae
723
-
 adenosine 5'-tetraphosphate
-
 Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.8
-
-
 Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
3.8 7.2 50% of maximal activity at pH 3.8 and 7.2 Saccharomyces cerevisiae