Literature summary for 3.6.1.13 extracted from

Njuguna, E.; Coussens, E.G.; Neyt, P.; Aesaert, S.; Storme, V.; Demuynck, K.; Vanhaeren, H.; Dhondt, S.; van Haver, Y.; Paul, L.; Inze, D.; Nelissen, H.; van Lijsebettens, M.
Functional analysis of Arabidopsis and maize transgenic lines overexpressing the ADP-ribose/NADH pyrophosphohydrolase, AtNUDX7 (2019), Int. J. Dev. Biol., 63, 45-55 .

Cloned(Commentary)

Cloned (Comment)	Organism
gene AtNUDX7, overexpression of ADP-ribose/NADH pyrophosphohydrolase, AtNUDX7, in several Arabidopsis thaliana and Zea mays transgenic lines under control of the constitutive caulixadflower Mosaic virus 35S promoter and the strong constitutive Brachypodium distachyon pBdEF1a promoter, respectively, quantitative PCR expression analysis. Lines containing the pZmUBIL::AtNUDX7 overexpression construct have generally much lower expression levels than lines driven by the pBdEF1a promoter	Arabidopsis thaliana

Cloned (Comment)

Organism

gene AtNUDX7, overexpression of ADP-ribose/NADH pyrophosphohydrolase, AtNUDX7, in several Arabidopsis thaliana and Zea mays transgenic lines under control of the constitutive caulixadflower Mosaic virus 35S promoter and the strong constitutive Brachypodium distachyon pBdEF1a promoter, respectively, quantitative PCR expression analysis. Lines containing the pZmUBIL::AtNUDX7 overexpression construct have generally much lower expression levels than lines driven by the pBdEF1a promoter

Arabidopsis thaliana

Protein Variants

Protein Variants	Comment	Organism
additional information	overexpression of AtNUDX7 in Arabidopsis thaliana improves seed parameters, accelerates flowering, and reduces inflorescence height. AtNUDX7 overexpression in Arabixaddopsis might enhance the ADP-ribose recycling step and maintain energy levels by supplying an ATP source in the poly(ADP-ribosyl)ation energy homeostasis pathway. Arabidopsis and maize lines with high, medium and low overexpression levels of the AtNUDX7 gene are analysed in automated platforms and the inhibition of several growth parameters is determined under mild drought stress conditions. The constitutive overexpression of the Arabidopxadsis AtNUDX7 gene in Arabidopsis and maize at varying levels does not improve tolerance to mild drought stress, but knocking down AtNUDX7 expression does, however at the expense of general growth under normal conditions. A loss-of-function mutant line, designated Atnudx7-1 (SALK-046441), has a T-DNA insertion in exon 1 of the AtNUDX7 gene, in the Col-0 background and verified for its T-DNA insertion position, homozygous T-DNA insertion, AtNUDX7 gene expression, and is used as a negative control. Evaluation of the AtNUDX7 overexpression maize lines under mild drought stress	Arabidopsis thaliana

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Arabidopsis thaliana	5829	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
ADP-alpha-D-ribose + H2O	Arabidopsis thaliana	-	AMP + D-ribose 5-phosphate	-	?
ADP-alpha-D-ribose + H2O	Arabidopsis thaliana Col-0	-	AMP + D-ribose 5-phosphate	-	?
NADH + H2O	Arabidopsis thaliana	-	AMP + NMNH	-	?
NADH + H2O	Arabidopsis thaliana Col-0	-	AMP + NMNH	-	?

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	Q9SU14	-	-
Arabidopsis thaliana Col-0	Q9SU14	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
ADP-alpha-D-ribose + H2O	-	Arabidopsis thaliana	AMP + D-ribose 5-phosphate	-	?
ADP-alpha-D-ribose + H2O	-	Arabidopsis thaliana Col-0	AMP + D-ribose 5-phosphate	-	?
NADH + H2O	-	Arabidopsis thaliana	AMP + NMNH	-	?
NADH + H2O	-	Arabidopsis thaliana Col-0	AMP + NMNH	-	?

Synonyms

Synonyms	Comment	Organism
ADP-ribose/NADH pyrophosphohydrolase	-	Arabidopsis thaliana
AT4G12720	locus name	Arabidopsis thaliana
AtNUDX7	-	Arabidopsis thaliana
Nudix hydrolase 7	-	Arabidopsis thaliana

Expression

Organism	Comment	Expression
Arabidopsis thaliana	AtNUDX7 is induced by multiple stresses and is involved in both biotic and abiotic stress responses	up

General Information

General Information	Comment	Organism
malfunction	improved seed yield parameters and early flowering time upon overexpression of AtNUDX7 in Arabidopsis thaliana. Different levels of constitutive overexpresxadsion of the Arabidopsis AtNUDX7 gene in Arabidopsis and also in maize do not result in a mild drought stress tolerance phenotype. But downregulation of AtNUDX7 results in mild drought stress tolerance under water deficit but growth under normal conditions is reduced, phenotypes, overview	Arabidopsis thaliana
metabolism	the poly(ADP-ribosyl)ation (PAR) pathway is a postxadtranslational protein modification process, activated upon single- or double-stranded DNA breaks, in which ADP-ribose subunits from nicotinamide adenine dinucleotide (NAD+) are covalently attached to target proteins mediated by the poly(ADP-ribose) polymerase enzyme (PARP, EC 2.4.2.30). PARP activity can be reversed by a poly(ADP-ribose) glycohydrolase enzyme (PARG, EC 3.2.1.143) generating free ADP-ribose molecules that can be degraded into adenosine monophosphate (AMP) and ribose-5-phosphate by the ADP-ribose-specific Nudix hydrolase enzyme. AMP can be utilized to replenish the ATP and NAD+, leading to maintenance of cellular homeostasis. Cytosolic enzymes AtNUDX2, AtNUDX6, AtNUDX7, and AtNUDX10 have pyrophosphohydrolase activity toward both ADP-ribose and NADH. Role of the poly(ADP-ribosyl)ation pathway in stress response and energy homeostasis, overview	Arabidopsis thaliana