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Literature summary for 3.6.1.13 extracted from

  • Okazaki, N.; Adachi, M.; Tamada, T.; Kurihara, K.; Ooga, T.; Kamiya, N.; Kuramitsu, S.; Kuroki, R.
    Crystallization and preliminary neutron diffraction studies of ADP-ribose pyrophosphatase-I from Thermus thermophilus HB8 (2012), Acta Crystallogr. Sect. F, 68, 49-52.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Thermus thermophilus

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with alpha,beta-methyleneadenosine diphosphoribose, sitting drop vapor diffusion method, using 18% (w/v) PEG 4000, 0.1 M sodium acetate buffer pH 5.3, 20% (w/v) glycerol, 0.2 M ammonium sulfate, at 20°C Thermus thermophilus

Localization

Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Thermus thermophilus
Zn2+ required Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Thermus thermophilus Q84CU3
-
-
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 Q84CU3
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Toyopearl Phenyl-650M column chromatography, and Q Sepharose column chromatography Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP-ribose + H2O highly specific substrate Thermus thermophilus AMP + D-ribose 5-phosphate
-
 ?
ADP-ribose + H2O highly specific substrate Thermus thermophilus HB8 / ATCC 27634 / DSM 579 AMP + D-ribose 5-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
ADP-ribose pyrophosphatase-I
-
 Thermus thermophilus
ADPRase-I
-
 Thermus thermophilus
Ndx4
-
 Thermus thermophilus