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Literature summary for 3.6.1.13 extracted from

  • Yang, H.; Slupska, M.M.; Wei, Y.F.; Tai, J.H.; Luther, W.M.; Xia, Y.R.; Shih, D.M.; Chiang, J.H.; Baikkalov, C.; Fitz-Gibbon, S.; Phan, I.T.; Conrad, A.; Miller, J.H.
    Cloning and characterization of a new member of nudix hydrolases from human and mouse (2000), J. Biol. Chem., 275, 8844-8853.
    View publication on PubMed
Search for more literature for 3.6.1.13

Cloned(Commentary)

Cloned (Comment) Organism
hexahistidine-tagged recombinant enzyme expressed in Escherichia coli Homo sapiens
hexahistidine-tagged recombinant enzyme expressed in Escherichia coli Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.032
-
 ADP-ribose pH 7.0, 37°C Homo sapiens
0.038
-
 ADP-ribose pH 9.0, 37°C Mus musculus
0.083
-
 ADP-mannose pH 7.0, 37°C Homo sapiens
0.154
-
 ADP-mannose pH 9.0, 37°C Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 1 mM required for optimal activity Homo sapiens
Mg2+ 5 mM required for optimal activity Mus musculus
Mn2+ can partially substitute for Mg2+ Homo sapiens
Mn2+ can partially substitute for Mg2+ Mus musculus
Zn2+ can partially substitute for Mg2+ Homo sapiens
Zn2+ can partially substitute for Mg2+ Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 x * 40000, SDS-PAGE Mus musculus
40000
-
 x * 40000, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9UKK9
-
-
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Homo sapiens
recombinant enzyme Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
A-549 cell
-
 Homo sapiens
-
adrenal gland
-
 Homo sapiens
-
amygdala
-
 Homo sapiens
-
bone marrow
-
 Homo sapiens
-
brain
-
 Homo sapiens
-
brain
-
 Mus musculus
-
Burkitt lymphoma cell Raji and Daudi Homo sapiens
-
caudate nucleus
-
 Homo sapiens
-
caudate putamen
-
 Homo sapiens
-
cerebellum
-
 Homo sapiens
-
cerebral cortex
-
 Homo sapiens
-
colon
-
 Homo sapiens
-
duodenum
-
 Homo sapiens
-
esophagus
-
 Homo sapiens
-
frontal lobe
-
 Homo sapiens
-
heart
-
 Mus musculus
-
HeLa cell S3 Homo sapiens
-
hippocampus
-
 Homo sapiens
-
HL-60 cell
-
 Homo sapiens
-
ileocecum
-
 Homo sapiens
-
ileum
-
 Homo sapiens
-
jejunum
-
 Homo sapiens
-
K-562 cell
-
 Homo sapiens
-
kidney
-
 Homo sapiens
-
kidney
-
 Mus musculus
-
leukocyte peripheral blood Homo sapiens
-
liver
-
 Homo sapiens
-
liver
-
 Mus musculus
-
lung
-
 Homo sapiens
-
lung
-
 Mus musculus
-
lymph node
-
 Homo sapiens
-
mammary gland
-
 Homo sapiens
-
medulla oblongata
-
 Homo sapiens
-
MOLT-4 cell
-
 Homo sapiens
-
occipital lobe
-
 Homo sapiens
-
pancreas
-
 Homo sapiens
-
parietal lobe
-
 Homo sapiens
-
pituitary gland
-
 Homo sapiens
-
placenta
-
 Homo sapiens
-
pons
-
 Homo sapiens
-
prostate
-
 Homo sapiens
-
rectum
-
 Homo sapiens
-
salivary gland
-
 Homo sapiens
-
skeletal muscle
-
 Mus musculus
-
spinal cord
-
 Homo sapiens
-
spleen
-
 Homo sapiens
-
spleen
-
 Mus musculus
-
stomach
-
 Homo sapiens
-
substantia nigra weak Homo sapiens
-
SW-480 cell
-
 Homo sapiens
-
testis
-
 Homo sapiens
-
testis
-
 Mus musculus
-
thalamus weak Homo sapiens
-
thymus
-
 Homo sapiens
-
thyroid gland
-
 Homo sapiens
-
trachea
-
 Homo sapiens
-
vermiform appendix
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP-glucose + H2O about 70% of the activity with ADP-mannose Homo sapiens AMP + alpha-D-glucose 1-phosphate
-
 ?
ADP-glucose + H2O about 70% of the activity with ADP-ribose Mus musculus AMP + alpha-D-glucose 1-phosphate
-
 ?
ADP-mannose + H2O about 80% of the activity with ADP-ribose Mus musculus AMP + D-mannose 1-phosphate
-
 ?
ADP-mannose + H2O
-
 Homo sapiens AMP + D-mannose 1-phosphate
-
 ?
ADP-ribose + H2O about 65% of the activity with ADP-mannose Homo sapiens AMP + ribose 5-phosphate
-
 ?
ADP-ribose + H2O
-
 Mus musculus AMP + ribose 5-phosphate
-
 ?
GDP-glucose + H2O about 15% of the activity with ADP-mannose Homo sapiens GMP + glucose 5-phosphate
-
 ?
GDP-glucose + H2O about 15% of the activity with ADP-ribose Mus musculus GMP + glucose 5-phosphate
-
 ?
GDP-mannose + H2O about 15% of the activity with ADP-mannose Homo sapiens GMP + D-mannose 1-phosphate
-
 ?
GDP-mannose + H2O about 20% of the activity with ADP-ribose Mus musculus GMP + D-mannose 1-phosphate
-
 ?
NADH + H2O about 12% of the activity with ADP-mannose Homo sapiens AMP + NMNH
-
 ?
NADH + H2O about 15% of the activity with ADP-ribose Mus musculus AMP + NMNH
-
 ?
UDP-glucose + H2O about 12% of the activity with ADP-mannose Homo sapiens UMP + glucose 5-phosphate
-
 ?
UDP-glucose + H2O about 5% of the activity with ADP-ribose Mus musculus UMP + glucose 5-phosphate
-
 ?
UDP-mannose + H2O about 15% of the activity with ADP-mannose Homo sapiens UMP + D-mannose 1-phosphate
-
 ?
UDP-mannose + H2O about 5% of the activity with ADP-ribose Mus musculus UMP + D-mannose 1-phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 40000, SDS-PAGE Homo sapiens
? x * 40000, SDS-PAGE Mus musculus

Synonyms

Synonyms Comment Organism
ADP-ribose pyrophosphatase
-
 Homo sapiens
ADP-ribose pyrophosphatase
-
 Mus musculus
mNUDT5 protein
-
 Mus musculus
NUDT5 protein
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 9 activity at pH 8.0 is about 90% of the activity at pH 6.5 and at pH 9.0, activity with ADP-ribose Homo sapiens
7
-
 activity with ADP-mannose Homo sapiens
9
-
 activity with ADP-mannose or ADP-ribose Mus musculus

pH Range

pH Minimum pH Maximum Comment Organism
4.5 10 pH 4.5: about 55% of maximal activity, pH 10.0: about 50% of maximal activity, activity with ADP-mannose Homo sapiens
6 10 pH 6.0: about 25% of maximal activity, pH 10.0: about 60% of maximal activity, activity with ADP-ribose Homo sapiens
7.5 10 pH 7.5: about 60% of maximal activity with ADP-ribose, about 50% of maximal activity with ADP-mannose, pH 10.0: about 70% of maximal activity with activity with ADP-ribose or ADP-mannose Mus musculus

pI Value

Organism Comment pI Value Maximum pI Value
Homo sapiens
-
-
 4.9
Mus musculus
-
-
 4.9