Any feedback?
Literature summary for 3.6.1.13 extracted from
- Cloning, expression and characterization of YSA1H, a human adenosine 5'-diphosphosugar pyrophosphatase possessing a MutT motif (1999), Biochem. J., 344, 331-337.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression as a thioredoxin fusion protein in Escherichia coli | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| F- | IC50: 0.02 mM | Homo sapiens |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.06 | - |
ADP-ribose | pH 7.5, 37°C | Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | optimal activity with 2.5-5 mM Mg2+ or 0.1-0.25 mM Mn2+ | Homo sapiens | |
| Mn2+ | optimal activity with 2.5-5 mM Mg2+ or 0.1-0.25 mM Mn2+ | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24300 | - |
2 * 24300, native protein, mass spectrometry | Homo sapiens |
| 85000 | - |
dimeric thioredoxin fusion protein expressed in Escherichia coli, gel filtration | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP-ribose + H2O | Homo sapiens | the function of the enzyme might be to remove free ADP-ribose arising from NAD+ and protein-bound poly- and non-enzymic protein glycation | AMP + ribose 5-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q9UKK9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adrenal gland | - |
Homo sapiens | - |
| aorta | - |
Homo sapiens | - |
| bladder | - |
Homo sapiens | - |
| bone marrow | low activity | Homo sapiens | - |
| brain | - |
Homo sapiens | - |
| caudate nucleus | - |
Homo sapiens | - |
| caudate putamen | - |
Homo sapiens | - |
| cerebellum | - |
Homo sapiens | - |
| cerebral cortex | - |
Homo sapiens | - |
| colon | - |
Homo sapiens | - |
| frontal lobe | - |
Homo sapiens | - |
| heart | - |
Homo sapiens | - |
| hippocampus | - |
Homo sapiens | - |
| kidney | high activity | Homo sapiens | - |
| leukocyte | - |
Homo sapiens | - |
| liver | high activity | Homo sapiens | - |
| lung | - |
Homo sapiens | - |
| lymph node | low activity | Homo sapiens | - |
| mammary gland | - |
Homo sapiens | - |
| medulla oblongata | - |
Homo sapiens | - |
| nucleus accumbens | - |
Homo sapiens | - |
| occipital lobe | - |
Homo sapiens | - |
| ovary | - |
Homo sapiens | - |
| pancreas | - |
Homo sapiens | - |
| peripheral blood | - |
Homo sapiens | - |
| pituitary gland | high activity | Homo sapiens | - |
| placenta | high activity | Homo sapiens | - |
| prostate | - |
Homo sapiens | - |
| salivary gland | - |
Homo sapiens | - |
| skeletal muscle | - |
Homo sapiens | - |
| small intestine | - |
Homo sapiens | - |
| spinal cord | - |
Homo sapiens | - |
| spleen | - |
Homo sapiens | - |
| stomach | - |
Homo sapiens | - |
| substantia nigra | - |
Homo sapiens | - |
| temporal lobe | - |
Homo sapiens | - |
| testis | - |
Homo sapiens | - |
| thalamus | - |
Homo sapiens | - |
| thymus | high activity | Homo sapiens | - |
| thyroid gland | - |
Homo sapiens | - |
| trachea | - |
Homo sapiens | - |
| uterus | - |
Homo sapiens | - |
| vermiform appendix | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP-glucose + H2O | 56% of the activity with ADP-ribose | Homo sapiens | AMP + alpha-D-glucose 1-phosphate | - |
? | |
| ADP-mannose + H2O | 103% of the activity with ADP-ribose | Homo sapiens | AMP + D-mannose 1-phosphate | - |
? | |
| ADP-ribose + H2O | the function of the enzyme might be to remove free ADP-ribose arising from NAD+ and protein-bound poly- and non-enzymic protein glycation | Homo sapiens | AMP + ribose 5-phosphate | - |
? | |
| ADP-ribose + H2O | - |
Homo sapiens | AMP + ribose 5-phosphate | - |
? | |
| diadenosine 5',5''-diphosphate + H2O | 20% the activity with ADP-ribose | Homo sapiens | ? | - |
? | |
| GDP-glucose + H2O | 7% of the activity with ADP-ribose | Homo sapiens | GMP + glucose 5-phosphate | - |
? | |
| IDP-ribose + H2O | 9% of the activity with ADP-ribose | Homo sapiens | IMP + ribose 5-phosphate | - |
? | |
| NAD+ + H2O | 7% of the activity with ADP-ribose | Homo sapiens | AMP + NMN | - |
? | |
| NADH + H2O | 7% of the activity with ADP-ribose | Homo sapiens | AMP + NMNH | - |
? | |
| NADPH + H2O | 12% of the activity with ADP-ribose | Homo sapiens | AMP + ? | - |
? | |
| UDP-galactose + H2O | 7% of the activity with ADP-ribose | Homo sapiens | UDP + galactose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 24300, native protein, mass spectrometry | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| adenosine 5'-diphosphosugar pyrophosphatase | - |
Homo sapiens |
| YSA1H | - |
Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.5 | - |
ADP-ribose | pH 7.5, 37°C | Homo sapiens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | 9 | with 2.5-5 mM Mg2+ or 0.1-0.25 mM Mn2+ | Homo sapiens |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
IC50: 0.02 mM | Homo sapiens | F- | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2026.1 (March 2026)
Legal
Curated at
Member of
![DSMZ Digital Diversity]()
![Global Core Biodata Resource]()
![ELIXIR Core Data Resource]()
![German Network for Bioinformatics Infrastructure]()
