KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0005 | 0.001 | ADPribose | ADPRibase I | Rattus norvegicus | |
0.015 | 0.03 | ADPribose | ADPRibase-Mn | Rattus norvegicus | |
0.05 | 0.1 | ADPribose | ADPRibase II | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | ADPRibase-I is highly specific for ADPribose and IDPribose in presence of Mg2+, but active also on non-reducing ADP-hexoses and dinucleotides when Mg2+ is replaced with Mn2+. ADPRibase-Mn is a Mn2+-dependent diphosphatase active on ADPribose and IDPribose, dinucleotides and CDP alcohols. ADPribase-II is a rather unspecific diphosphatase that, with Mg2+, is active on AMP or IMP containing NDP sugars and dinucleotides, and with Mn2+ also active on non-adenine NDP-sugars and CDP-alcohols | Rattus norvegicus | |
Mn2+ | ADPRibase-I is highly specific for ADPribose and IDPribose in presence of Mg2+, but active also on non-reducing ADP-hexoses and dinucleotides when Mg2+ is replaced with Mn2+. ADPRibase-Mn is a Mn2+-dependent diphosphatase active on ADPribose and IDPribose, dinucleotides and CDP alcohols. ADPribase-II is a rather unspecific diphosphatase that, with Mg2+, is active on AMP or IMP containing NDP sugars and dinucleotides, and with Mn2+ also active on non-adenine NDP-sugars and CDP-alcohols | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
ADPRibase-Mn, gel filtration | Rattus norvegicus |
40000 | - |
ADPRibase I, gel filtration | Rattus norvegicus |
47000 | - |
ADPRibase II, gel filtration | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ADPRibaseI, ADPribase II and ADPRibase-Mn | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.439 | - |
ADPRibase I | Rattus norvegicus |
12.81 | - |
ADPRibase II | Rattus norvegicus |
18.2 | - |
ADPRibase-Mn | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP-glucose + H2O | ADPRibase II, very low activity with ADPRibase I | Rattus norvegicus | AMP + alpha-D-glucose 1-phosphate | - |
? | |
ADP-mannose + H2O | ADPRibase II, low activity with ADPRibase I | Rattus norvegicus | AMP + D-mannose 1-phosphate | - |
? | |
ADPribose + H2O | - |
Rattus norvegicus | AMP + D-ribose 5-phosphate | - |
? | |
FAD + H2O | ADPRibase-Mn and ADPRibase II, very low activity with ADPRibase I in presence of Mn2+ | Rattus norvegicus | ? | - |
? | |
IDP-ribose + H2O | - |
Rattus norvegicus | IMP + ribose 5-phosphate | - |
? | |
additional information | three diphosphatases: 1.ADPRibase-I: an ADP-ribose diphosphatase highly specific for ADPribose and IDPribose in presence of Mg2+, but active also on non-reducing ADP-hexoses and dinucleotides, not on NAD+, when Mg2+ is replaced with Mn2+, 2. ADPRibase-Mn: a Mn2+-dependent diphosphatase active on ADPribose and IDPribose, dinucleotides and CDP alcohols, 3. ADPribase-II: a rather unspecific diphosphatase that, with Mg2+, is active on AMP or IMP containing NDP sugars and dinucleotides, not on NAD+, and with Mn2+ also active on non-adenine NDP-sugars and CDP-alcohols | Rattus norvegicus | ? | - |
? | |
NAD+ + H2O | ADPRibase-Mn in presence of Mn2+, no activity with ADPRibase I and ADPRibase II | Rattus norvegicus | ? | - |
? | |
NADH + H2O | ADPRibase I, ADPribase II and ADPRibase-Mn in presence of Mn2+ | Rattus norvegicus | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | ADPRibase I and ADPRibase II | Rattus norvegicus |
9 | - |
ADPRibase-Mn | Rattus norvegicus |