Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.6.1.13 extracted from

  • Canales, J.; Pinto, R.M.; Costas, M.J.; Hernandez, M.T.; Miro, A.; Bernet, D.; Fernandez, A.; Cameselle, J.C.
    Rat liver nucleoside diphosphosugar or diphosphoalcohol pyrophosphatase different from nucleotide pyrophosphatase or phosphodiesterase I: substrate specificities of Mg2+- and/or Mn2+-depeneent hydrolases acting on ADP-ribose (1995), Biochim. Biophys. Acta, 1246, 167-177.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0005 0.001 ADPribose ADPRibase I Rattus norvegicus
0.015 0.03 ADPribose ADPRibase-Mn Rattus norvegicus
0.05 0.1 ADPribose ADPRibase II Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ ADPRibase-I is highly specific for ADPribose and IDPribose in presence of Mg2+, but active also on non-reducing ADP-hexoses and dinucleotides when Mg2+ is replaced with Mn2+. ADPRibase-Mn is a Mn2+-dependent diphosphatase active on ADPribose and IDPribose, dinucleotides and CDP alcohols. ADPribase-II is a rather unspecific diphosphatase that, with Mg2+, is active on AMP or IMP containing NDP sugars and dinucleotides, and with Mn2+ also active on non-adenine NDP-sugars and CDP-alcohols Rattus norvegicus
Mn2+ ADPRibase-I is highly specific for ADPribose and IDPribose in presence of Mg2+, but active also on non-reducing ADP-hexoses and dinucleotides when Mg2+ is replaced with Mn2+. ADPRibase-Mn is a Mn2+-dependent diphosphatase active on ADPribose and IDPribose, dinucleotides and CDP alcohols. ADPribase-II is a rather unspecific diphosphatase that, with Mg2+, is active on AMP or IMP containing NDP sugars and dinucleotides, and with Mn2+ also active on non-adenine NDP-sugars and CDP-alcohols Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
ADPRibase-Mn, gel filtration Rattus norvegicus
40000
-
ADPRibase I, gel filtration Rattus norvegicus
47000
-
ADPRibase II, gel filtration Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ADPRibaseI, ADPribase II and ADPRibase-Mn Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.439
-
ADPRibase I Rattus norvegicus
12.81
-
ADPRibase II Rattus norvegicus
18.2
-
ADPRibase-Mn Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP-glucose + H2O ADPRibase II, very low activity with ADPRibase I Rattus norvegicus AMP + alpha-D-glucose 1-phosphate
-
?
ADP-mannose + H2O ADPRibase II, low activity with ADPRibase I Rattus norvegicus AMP + D-mannose 1-phosphate
-
?
ADPribose + H2O
-
Rattus norvegicus AMP + D-ribose 5-phosphate
-
?
FAD + H2O ADPRibase-Mn and ADPRibase II, very low activity with ADPRibase I in presence of Mn2+ Rattus norvegicus ?
-
?
IDP-ribose + H2O
-
Rattus norvegicus IMP + ribose 5-phosphate
-
?
additional information three diphosphatases: 1.ADPRibase-I: an ADP-ribose diphosphatase highly specific for ADPribose and IDPribose in presence of Mg2+, but active also on non-reducing ADP-hexoses and dinucleotides, not on NAD+, when Mg2+ is replaced with Mn2+, 2. ADPRibase-Mn: a Mn2+-dependent diphosphatase active on ADPribose and IDPribose, dinucleotides and CDP alcohols, 3. ADPribase-II: a rather unspecific diphosphatase that, with Mg2+, is active on AMP or IMP containing NDP sugars and dinucleotides, not on NAD+, and with Mn2+ also active on non-adenine NDP-sugars and CDP-alcohols Rattus norvegicus ?
-
?
NAD+ + H2O ADPRibase-Mn in presence of Mn2+, no activity with ADPRibase I and ADPRibase II Rattus norvegicus ?
-
?
NADH + H2O ADPRibase I, ADPribase II and ADPRibase-Mn in presence of Mn2+ Rattus norvegicus ?
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 9 ADPRibase I and ADPRibase II Rattus norvegicus
9
-
ADPRibase-Mn Rattus norvegicus