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Literature summary for 3.6.1.13 extracted from
- Identification and characterization of the nudix hydrolase from the archaeon, Methanococcus jannaschii, as a highly specific ADP-ribose pyrophosphatase (1998), J. Biol. Chem., 273, 20924-20928.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Methanocaldococcus jannaschii |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.34 | - |
ADPribose | - |
Methanocaldococcus jannaschii |  |
| 0.46 | - |
ADPribose 2'-phosphate | - |
Methanocaldococcus jannaschii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Methanocaldococcus jannaschii | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanocaldococcus jannaschii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Methanocaldococcus jannaschii |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| erythrocyte | - |
Methanocaldococcus jannaschii | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADPribose + H2O | - |
Methanocaldococcus jannaschii | AMP + D-ribose 5-phosphate | - |
? | |
| ADPribose 2'-phosphate + H2O | - |
Methanocaldococcus jannaschii | adenosine 2',5'-diphosphate + D-ribose 5-phosphate | - |
? | |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 75 | rate of hydrolysis of ADPribose is 15fold higher than at 37°C, incubations at higher temperatures are impractical because of the spontaneous hydrolysis of the substrate | Methanocaldococcus jannaschii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
5 min, retains full activity | Methanocaldococcus jannaschii |
| 85 | - |
5 min, 30% loss of activity | Methanocaldococcus jannaschii |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.14 | - |
ADPribose 2'-phosphate | - |
Methanocaldococcus jannaschii | |
| 1.98 | - |
ADPribose | - |
Methanocaldococcus jannaschii | |
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