Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Chlorobaculum tepidum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Chlorobaculum tepidum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0977 | - |
(phosphate)3 | pH 6.5, 25°C | Chlorobaculum tepidum | |
0.213 | - |
(phosphate)3 | pH 6.5, 25°C | Chlorobaculum tepidum | |
0.242 | - |
guanosine 5'-tetraphosphate | pH 6.5, 25°C | Chlorobaculum tepidum | |
0.264 | - |
(phosphate)13-18 | pH 6.5, 25°C | Chlorobaculum tepidum | |
0.335 | - |
guanosine 5'-tetraphosphate | pH 6.5, 25°C | Chlorobaculum tepidum | |
0.597 | - |
(phosphate)13-18 | pH 6.5, 25°C | Chlorobaculum tepidum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | 20 mM, about 3fold stimulation | Chlorobaculum tepidum | |
Mg2+ | best activator, maximum activity at 5 mM | Chlorobaculum tepidum | |
Mn2+ | 5 mM, about 37% of the activity with Mg2+ | Chlorobaculum tepidum | |
Mn2+ | 5 mM, about 65% of the activity with Mg2+ | Chlorobaculum tepidum | |
additional information | presence of divalent cation is absolutely required, Mg2+ is preferred | Chlorobaculum tepidum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
1 * 37000, SDS-PAGE | Chlorobaculum tepidum |
38800 | - |
gel filtration | Chlorobaculum tepidum |
59900 | - |
2 * 59900, SDS-PAGE | Chlorobaculum tepidum |
100400 | - |
gel filtration | Chlorobaculum tepidum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlorobaculum tepidum | Q8KBS0 | - |
- |
Chlorobaculum tepidum | Q8KG69 | - |
- |
Chlorobaculum tepidum DSM 12025 | Q8KBS0 | - |
- |
Chlorobaculum tepidum DSM 12025 | Q8KG69 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
15 | - |
substrate (phosphate)300, pH 6.5, 25°C | Chlorobaculum tepidum |
126 | - |
substrate (phosphate)300, pH 6.5, 25°C | Chlorobaculum tepidum |
170 | - |
substrate (phosphate)13-18, pH 6.5, 25°C | Chlorobaculum tepidum |
180 | - |
substrate (phosphate)13-18, pH 6.5, 25°C | Chlorobaculum tepidum |
590 | - |
substrate (phosphate)3, pH 6.5, 25°C | Chlorobaculum tepidum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(phosphate)13-18 + H2O | - |
Chlorobaculum tepidum | (phosphate)12-17 + phosphate | - |
? | |
(phosphate)13-18 + H2O | - |
Chlorobaculum tepidum DSM 12025 | (phosphate)12-17 + phosphate | - |
? | |
(phosphate)3 + H2O | - |
Chlorobaculum tepidum | (phosphate)2 + phosphate | - |
? | |
(phosphate)3 + H2O | best substrate | Chlorobaculum tepidum | (phosphate)2 + phosphate | - |
? | |
(phosphate)3 + H2O | - |
Chlorobaculum tepidum DSM 12025 | (phosphate)2 + phosphate | - |
? | |
(phosphate)3 + H2O | best substrate | Chlorobaculum tepidum DSM 12025 | (phosphate)2 + phosphate | - |
? | |
(phosphate)300 + H2O | or even longer chain length | Chlorobaculum tepidum | (phosphate)299 + phosphate | - |
? | |
(phosphate)300 + H2O | or even longer chain length | Chlorobaculum tepidum DSM 12025 | (phosphate)299 + phosphate | - |
? | |
guanosine 5'-tetraphosphate + H2O | - |
Chlorobaculum tepidum | GTP + phosphate | - |
? | |
guanosine 5'-tetraphosphate + H2O | - |
Chlorobaculum tepidum DSM 12025 | GTP + phosphate | - |
? | |
additional information | isoform Ppx1 shows substantial nucleoside triphosphatase activity | Chlorobaculum tepidum | ? | - |
? | |
additional information | isoform Ppx1 shows substantial nucleoside triphosphatase activity | Chlorobaculum tepidum DSM 12025 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 59900, SDS-PAGE | Chlorobaculum tepidum |
monomer | 1 * 37000, SDS-PAGE | Chlorobaculum tepidum |
Synonyms | Comment | Organism |
---|---|---|
CT0099 | - |
Chlorobaculum tepidum |
CT1713 | - |
Chlorobaculum tepidum |
PPX1 | - |
Chlorobaculum tepidum |
PPX2 | - |
Chlorobaculum tepidum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
13.5 | - |
(phosphate)3 | pH 6.5, 25°C | Chlorobaculum tepidum | |
14.2 | - |
guanosine 5'-tetraphosphate | pH 6.5, 25°C | Chlorobaculum tepidum | |
157 | - |
(phosphate)13-18 | pH 6.5, 25°C | Chlorobaculum tepidum | |
308 | - |
guanosine 5'-tetraphosphate | pH 6.5, 25°C | Chlorobaculum tepidum | |
399 | - |
(phosphate)3 | pH 6.5, 25°C | Chlorobaculum tepidum | |
458 | - |
(phosphate)13-18 | pH 6.5, 25°C | Chlorobaculum tepidum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Chlorobaculum tepidum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7.5 | more than 30-40% of maximum activity | Chlorobaculum tepidum |
10 | - |
about 90% of maximum activity | Chlorobaculum tepidum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
42 | - |
guanosine 5'-tetraphosphate | pH 6.5, 25°C | Chlorobaculum tepidum | |
63 | - |
(phosphate)3 | pH 6.5, 25°C | Chlorobaculum tepidum | |
263 | - |
(phosphate)13-18 | pH 6.5, 25°C | Chlorobaculum tepidum | |
1273 | - |
guanosine 5'-tetraphosphate | pH 6.5, 25°C | Chlorobaculum tepidum | |
1716 | - |
(phosphate)13-18 | pH 6.5, 25°C | Chlorobaculum tepidum | |
4081 | - |
(phosphate)3 | pH 6.5, 25°C | Chlorobaculum tepidum |