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Literature summary for 3.6.1.11 extracted from

  • Albi, T.; Serrano, A.
    Two exopolyphosphatases with distinct molecular architectures and substrate specificities from the thermophilic green-sulfur bacterium Chlorobium tepidum TLS (2014), Microbiology, 160, 2067-2078.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Chlorobaculum tepidum

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
Chlorobaculum tepidum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0977
-
(phosphate)3 pH 6.5, 25°C Chlorobaculum tepidum
0.213
-
(phosphate)3 pH 6.5, 25°C Chlorobaculum tepidum
0.242
-
guanosine 5'-tetraphosphate pH 6.5, 25°C Chlorobaculum tepidum
0.264
-
(phosphate)13-18 pH 6.5, 25°C Chlorobaculum tepidum
0.335
-
guanosine 5'-tetraphosphate pH 6.5, 25°C Chlorobaculum tepidum
0.597
-
(phosphate)13-18 pH 6.5, 25°C Chlorobaculum tepidum

Metals/Ions

Metals/Ions Comment Organism Structure
K+ 20 mM, about 3fold stimulation Chlorobaculum tepidum
Mg2+ best activator, maximum activity at 5 mM Chlorobaculum tepidum
Mn2+ 5 mM, about 37% of the activity with Mg2+ Chlorobaculum tepidum
Mn2+ 5 mM, about 65% of the activity with Mg2+ Chlorobaculum tepidum
additional information presence of divalent cation is absolutely required, Mg2+ is preferred Chlorobaculum tepidum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
1 * 37000, SDS-PAGE Chlorobaculum tepidum
38800
-
gel filtration Chlorobaculum tepidum
59900
-
2 * 59900, SDS-PAGE Chlorobaculum tepidum
100400
-
gel filtration Chlorobaculum tepidum

Organism

Organism UniProt Comment Textmining
Chlorobaculum tepidum Q8KBS0
-
-
Chlorobaculum tepidum Q8KG69
-
-
Chlorobaculum tepidum DSM 12025 Q8KBS0
-
-
Chlorobaculum tepidum DSM 12025 Q8KG69
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
15
-
substrate (phosphate)300, pH 6.5, 25°C Chlorobaculum tepidum
126
-
substrate (phosphate)300, pH 6.5, 25°C Chlorobaculum tepidum
170
-
substrate (phosphate)13-18, pH 6.5, 25°C Chlorobaculum tepidum
180
-
substrate (phosphate)13-18, pH 6.5, 25°C Chlorobaculum tepidum
590
-
substrate (phosphate)3, pH 6.5, 25°C Chlorobaculum tepidum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(phosphate)13-18 + H2O
-
Chlorobaculum tepidum (phosphate)12-17 + phosphate
-
?
(phosphate)13-18 + H2O
-
Chlorobaculum tepidum DSM 12025 (phosphate)12-17 + phosphate
-
?
(phosphate)3 + H2O
-
Chlorobaculum tepidum (phosphate)2 + phosphate
-
?
(phosphate)3 + H2O best substrate Chlorobaculum tepidum (phosphate)2 + phosphate
-
?
(phosphate)3 + H2O
-
Chlorobaculum tepidum DSM 12025 (phosphate)2 + phosphate
-
?
(phosphate)3 + H2O best substrate Chlorobaculum tepidum DSM 12025 (phosphate)2 + phosphate
-
?
(phosphate)300 + H2O or even longer chain length Chlorobaculum tepidum (phosphate)299 + phosphate
-
?
(phosphate)300 + H2O or even longer chain length Chlorobaculum tepidum DSM 12025 (phosphate)299 + phosphate
-
?
guanosine 5'-tetraphosphate + H2O
-
Chlorobaculum tepidum GTP + phosphate
-
?
guanosine 5'-tetraphosphate + H2O
-
Chlorobaculum tepidum DSM 12025 GTP + phosphate
-
?
additional information isoform Ppx1 shows substantial nucleoside triphosphatase activity Chlorobaculum tepidum ?
-
?
additional information isoform Ppx1 shows substantial nucleoside triphosphatase activity Chlorobaculum tepidum DSM 12025 ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 59900, SDS-PAGE Chlorobaculum tepidum
monomer 1 * 37000, SDS-PAGE Chlorobaculum tepidum

Synonyms

Synonyms Comment Organism
CT0099
-
Chlorobaculum tepidum
CT1713
-
Chlorobaculum tepidum
PPX1
-
Chlorobaculum tepidum
PPX2
-
Chlorobaculum tepidum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
13.5
-
(phosphate)3 pH 6.5, 25°C Chlorobaculum tepidum
14.2
-
guanosine 5'-tetraphosphate pH 6.5, 25°C Chlorobaculum tepidum
157
-
(phosphate)13-18 pH 6.5, 25°C Chlorobaculum tepidum
308
-
guanosine 5'-tetraphosphate pH 6.5, 25°C Chlorobaculum tepidum
399
-
(phosphate)3 pH 6.5, 25°C Chlorobaculum tepidum
458
-
(phosphate)13-18 pH 6.5, 25°C Chlorobaculum tepidum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Chlorobaculum tepidum

pH Range

pH Minimum pH Maximum Comment Organism
5.5 7.5 more than 30-40% of maximum activity Chlorobaculum tepidum
10
-
about 90% of maximum activity Chlorobaculum tepidum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
42
-
guanosine 5'-tetraphosphate pH 6.5, 25°C Chlorobaculum tepidum
63
-
(phosphate)3 pH 6.5, 25°C Chlorobaculum tepidum
263
-
(phosphate)13-18 pH 6.5, 25°C Chlorobaculum tepidum
1273
-
guanosine 5'-tetraphosphate pH 6.5, 25°C Chlorobaculum tepidum
1716
-
(phosphate)13-18 pH 6.5, 25°C Chlorobaculum tepidum
4081
-
(phosphate)3 pH 6.5, 25°C Chlorobaculum tepidum